SN/T 1999-2007 English PDF
Basic dataStandard ID: SN/T 1999-2007 (SN/T1999-2007)Description (Translated English): Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 7,772 Date of Issue: 2007-12-24 Date of Implementation: 2008-07-01 Quoted Standard: GB/T 6682 Regulation (derived from): ?Industry Standard Announcement 2008 No.2 (Total No.98) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the bovine viral diarrhea mucosal disease antigen capture enzyme-linked immunosorbent assay (ELISA) method of operation. This standard applies to bovine viral diarrhea mucosal disease antigen detection. SN/T 1999-2007: Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease Exit inspection and quarantine industry standard book People's Republic of China Bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent Assay procedures Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Dong Zhizhen, Hou Yanmei, Zhao Xiangping, Xiao Yan, Huo Lei. This standard is the first release of the entry-exit inspection and quarantine industry standards. Bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent Assay procedures1 ScopeThis standard specifies the bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent assay (ELISA) method of operation. This standard applies to bovine viral diarrhea - mucosal disease antigen detection.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (neq ISO 3696) Principle 3 To border disease virus-specific antibody-coated microtiter plates, while adding BVDV detection of monoclonal antibodies and the sample, the sample that is with two anti Binding, antigen-antibody conjugate is formed, the plate was washed and then secondary antibody (binding to an antigen-antibody complex) and the substrate, by measuring the optical density The determination results of samples.4 test material4.1 bovine viral diarrhea - mucosal disease antigen capture ELISA diagnostic kit 2 ℃ ~ 8 ℃ preservation. Kit should include the following items. a) bovine viral diarrhea - mucosal disease virus polyclonal antibody-coated plate; b) detection of anti-BVDV monoclonal antibodies; c) BVDV positive control; d) BVDV negative control; e) Horseradish peroxidase (HRP) labeled anti-mouse antibody cow; f) 10 × sample diluent; g) 10 × concentrated lotion; h) TMB substrate solution; i) Stop Solution. 4.2 sample handling 4.2.1 Organization 4.2.1.1 Use as fresh tissue samples, tissue can be stored for a month or long-term cryopreservation at 2 ℃ ~ 8 ℃. Most tissue samples Good for the tonsils, spleen, small intestine and lung. 4.2.1.2 using scissors to cut up the sample 1g ~ 2g (2mm ~ 5mm). 4.2.1.3 The sample was cut into 10mL centrifuge tube was added 5mL sample diluent (1 ×), mixing at room temperature for sense 1h ~ 2h. 4.2.1.4 1500r/min centrifugal 10min, supernatant as detected by draw 50μL sample. Chapter 7 test. 4.2.2 peripheral blood leukocytes 4.2.2.1 heparin or EDTA anticoagulated blood sample 10mL, 2000r/min centrifugal 15min ~ 20min. ...... |
