SN/T 1955-2007 English PDFUS$549.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1955-2007: Determination of stilbenes residues in foodstuffs of animal origin. Enzyme linked immunosorbent assay Status: Valid
Basic dataStandard ID: SN/T 1955-2007 (SN/T1955-2007)Description (Translated English): Determination of stilbenes residues in foodstuffs of animal origin. Enzyme linked immunosorbent assay Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.050 Word Count Estimation: 17,111 Date of Issue: 2007-08-06 Date of Implementation: 2008-03-01 Regulation (derived from): Accredidation-Technology-Letter [2015] No. 247 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the animal-derived foods stilbene hormone residues in the enzyme-linked immunoassay. This standard applies to chicken, fish, shrimp, and chicken liver hexoestrol, diethylstilbestrol residues detected. SN/T 1955-2007: Determination of stilbenes residues in foodstuffs of animal origin. Enzyme linked immunosorbent assay---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of stilbenes residues in foodstuffs of animal origin.Enzyme linked immunosorbent assay Exit inspection and quarantine industry standard book People's Republic of China Food of animal origin stilbene hormone residues in Detection ELISA Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordAppendix A of this standard and Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Sun Li, Kit Cheng, Tang Dan Zhou, Wei Yadong, Zhang Hongwei. This standard is the first release of the entry-exit inspection and quarantine industry standards. Food of animal origin stilbene hormone residues in Detection ELISA1 ScopeThis standard specifies the foods of animal origin enzyme immunoassay method stilbene of hormone residues. This standard applies to chicken, fish, shrimp and chicken liver hexoestrol, diethylstilbestrol residues detected.2 Sample preparation and preservation2.1 Preparation of the sample Remove all the samples taken from a representative sample of about 1kg, fully crush, mix, use quartering the sample into two equal parts, installed Into a clean container, sealed and do identity. Save 2.2 sample Sample was placed at -20 ℃ ~ -18 ℃ conditions of preservation.3 Determination3.1 Method summary Determination of the basis of this method is a competitive enzyme immunoassay. Microtiter plates had been coated with antibodies are estrone, and estrone with hexane, diethylstilbestrol Crosslinking reaction, standard or sample of estrone and estrone horseradish peroxidase-labeled antigen compete for common binding site on the antibody estrone Point, the absorbance value, estrone concentration is inversely proportional to pore solution measured using a microplate reader and the absorbance values of the calibration curve plotted by quantitative calculations. 3.2 Reagents and materials Unless otherwise specified, the use of chemical reagents were of analytical grade or higher level. Water re-distilled water. 3.2.1 stilbene immunoassay kit (see Appendix A). 3.2.2 tert-butyl methyl ether. chromatography. 3.2.3 chloroform. 3.2.4 6mol/L phosphate. lessons 58.8mL phosphoric acid is dissolved in water, and set the volume to 100mL. 3.2.5 ethanol. 3.2.6 β- glucuronidase (SigmaG-0876). 3.2.7 Sodium acetate (CH3COONa · 3H2O). 3.2.8 sodium acetate buffer (0.1mol/L, pH = 5.0). Weigh 13.6g of sodium acetate were dissolved in 800mL water, neutralized with sodium hydroxide solution Solution pH is adjusted to 5.0 ± 0.1, add water to 1000mL. 3.2.9 sodium hydroxide solution (1mol/L). Weigh 40g of sodium hydroxide dissolved in 1000mL of water. 3.2.10 methanol. 3.2.11 Standard. hexoestrol standards, diethylstilbestrol standard purity ≥98%. 3.2.12 formulated estrone standard solution. Accurately weigh an appropriate amount of hexane estrone and diethylstilbestrol standards, prepared in methanol 1mg/mL standard stock solution, store at 4 ℃ ~ 8 ℃ condition. 3.3 instruments and equipment 3.3.1 microplate reader. a wavelength of 450nm. 3.3.2 37 ℃ ± 2 ℃ incubator. 3.3.3 homogenizer. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1955-2007_English be delivered?Answer: Upon your order, we will start to translate SN/T 1955-2007_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1955-2007_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1955-2007_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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