SN/T 1840-2006 English PDF

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SN/T 1840-2006: Method for detection of plant viruses by immuno-electron microscopy
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1840-2006	199	Add to Cart	3 days	Method for detection of plant viruses by immuno-electron microscopy	Valid

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Basic data

Standard ID: SN/T 1840-2006 (SN/T1840-2006)
Description (Translated English): Method for detection of plant viruses by immuno-electron microscopy
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020
Word Count Estimation: 6,694
Date of Issue: 2006-11-10
Date of Implementation: 2007-05-16
Regulation (derived from): Chinese industry standards for record 2007 of 3 (total of 87)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the plant quarantine of plant viruses immune electron microscopy detection methods. This standard applies to plant seeds, seedlings and other plant propagation materials Kazakhstan products of plant viruses immune electron microscopy.

SN/T 1840-2006: Method for detection of plant viruses by immuno-electron microscopy

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for detection of plant viruses by immuno-electron microscopy Book of the People's Republic of China Entry and Exit Inspection and Quarantine Plant virus immunoelectron microscopy Released on.2006-11-10 2007-05-16 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration. This standard is drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Chen Zhinan, Zheng Yi, Deng Qiong, Wang Wu, Gu Guangyu. This standard was first published Inspection and quarantine industry standards. Plant virus immunoelectron microscopy

1 Scope

This standard specifies the immunoelectron microscopy method for plant viruses in plant quarantine. This standard is applicable to the immunoelectron microscopy of plant viruses in plant seeds, seedlings and other reproductive materials and plant products.

2 Principle

Immuno-electron microscopy (IEM) is a combination of immunological principles and electron microscopy. by The antibody coated on the copper network specifically and efficiently captures the virion from the crude extract of the plant tissue containing the virus, and is stained with uranium acetate. The morphological structure of the mitochondria and its external antibody halo can be clearly observed under transmission electron microscopy for the planting at the ultrastructural level. Identification of the virus provides information on the shape and serological properties of the antigen.

3 instruments and equipment

3.1 Micro juicer. 3.2 High speed refrigerated centrifuge. 3.3 Plant light incubator (temperature adjustable range 0 °C ~ 50 °C). 3.4 Electronic analytical balance (sensitivity. 0.001g). 3.5 pH meter (measurement accuracy. ±0.05 pH). 3.6 Transmission electron microscope (magnification..200,000 times). 3.7 mortar, centrifuge tube (1.5mL), copper mesh (400 mesh), dropper, pointed tweezers, Whatman filter paper, plastic petri dish, clean glass Film, Parafilm.

4 reagents and solutions

4.1 Sample Extraction Buffer Mix 50 mL of 0.1 mol/LTris base solution with 17.2 mL of 0.1 mol/L hydrochloric acid (HCl), and then add sucrose 13.68 g. And sodium chloride (NaCl) 0.88g, dilute to 100mL with sterile distilled water, formulated into 0.05mol/LTris-HCl (pH 8.4) sample extraction Buffer. Mix 50 mL of 0.1 mol/LTris base solution with 44.7 mL of 0.1 mol/L hydrochloric acid (HCl), and dilute to volume with sterile distilled water until 100 mL was prepared to a concentration of 0.05 mol/LTris-HCl buffer (pH 7.2). 4.3 phosphate buffer 57.7 mL of a solution of 1 mol/L disodium hydrogen phosphate (Na2HPO4) and a concentration of 1 mol/L sodium dihydrogen phosphate (NaH2PO4) solution 42.3mL was evenly mixed, added to sterilized distilled water to a volume of 1000mL, formulated into 0.1mol/L phosphate buffer Mother liquor (pH 7.0). When used, the mother liquid is diluted 5 times with sterilized distilled water to prepare a 0.02 mol/L phosphate buffer (pH 7.0). spare. The polyvinyl formal is dissolved in chloroform, formulated into a 0.25% solution, and stored in a refrigerator for use. Take a clean glass when making a film The glass piece is inserted into the solution, taken out and placed obliquely. After the chloroform is volatilized, a film is formed on the glass piece, and the tip of the glass is cut along the edge of the glass. The film is then immersed in sterilized distilled water, and the film is detached from the glass sheet and floats on the surface of the water to obtain a clean copper mesh surface. Gently place it on top, press it tightly, cover it with a piece of Parafilm, pick it up, place it in a Petri dish, and dry it for later use. ......

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