SN/T 1820-2006 English PDFUS$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1820-2006: Identification of Phytophthora cryptogea Pethybridge and Lafferty Status: Valid
Basic dataStandard ID: SN/T 1820-2006 (SN/T1820-2006)Description (Translated English): Identification of Phytophthora cryptogea Pethybridge and Lafferty Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16;C62 Classification of International Standard: 65.020 Word Count Estimation: 10,160 Date of Issue: 2006-11-10 Date of Implementation: 2007-05-16 Regulation (derived from): Chinese industry standards for record 2007 of 3 (total of 87) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the export plant quarantine implicitly Phytophthora quarantine and identification methods. This standard applies to the entry and exit of plants and soil media implicitly quarantine and identification of Phytophthora. SN/T 1820-2006: Identification of Phytophthora cryptogea Pethybridge and Lafferty---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Identification of Phytophthora cryptogea Pethybridge and Lafferty Book of the People's Republic of China Entry and Exit Inspection and Quarantine Phytosanitary identification method for Phytophthora Released on.2006-11-10 2007-05-16 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordAppendix A, Appendix B, Appendix C and Appendix D of this standard are informative annexes. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Tan Qunying, Liao Li, Yu Guoquan, Chen Qiwen, Zhang Weidong. This standard is the first industry standard for entry-exit inspection and quarantine. Phytosanitary identification method for Phytophthora1 ScopeThis standard stipulates the quarantine and identification methods of Phytophthora infestans in entry and exit plant quarantine. This standard is applicable to the quarantine and identification of Phytophthora infestans in inbound and outbound plants and medium soils.2 Principle2.1 Classification status 2.2 Quarantine principle The host plant of Phytophthora sinensis is very wide, and it is harmful to hundreds of economic crops. It is gerbera, aster, carnation, Christmas flower, tomato and other plants. Important pathogens (see Appendix A). Mainly with strong anti-reverse oospores in the medium soil and host residues in winter and long-term survival, When the field temperature and humidity are appropriate, the oospores break the dormant germination and produce sporangia. Sporangia soon after rain or after irrigation The release of a large number of zoospores, eventually invading the host through the roots or wounds, can cause roots and stems to rot, causing the plants to be infested after infection Wilted and died. 2.3 Identification basis The symptoms of the Phytophthora infestans on the host plant, the morphological characteristics of the pathogen, and the biological characteristics are the basis for identifying the bacteria.3 instruments and laboratory equipment3.1 Equipment Biological microscope, stereo microscope, autoclave, dry heat sterilizer, ordinary balance, analytical balance, ultra-clean workbench, biological incubator. 3.2 Laboratory equipment Alcohol lamps, medical scissors, tweezers, beakers, petri dishes, slides, coverslips, flasks, measuring cylinders, straws, jars.4 reagentsAmpicillin, pentachloronitrobenzene (PCNB), sodium hypochlorite (NaClO), benomyl, carbendazim (carbendazim), calcium nitrate [Ca(NO3)2], potassium dihydrogen phosphate (KH2PO4), magnesium nitrate [Mg(NO3)2], calcium chloride (CaCl2), Rampampicin, calcium carbonate (CaCO3), ethanol.5 medium5.1 Basic medium It is used for observation and measurement of preservation and culture traits of Phytophthora sinensis. Use carrot medium (CA), V8 medium (V8A) or lima bean medium (LBA), the preparation method and steps are shown in section B. Chapter 1. 5.2 Selective medium It is used for the germination of zoospores and the isolation and purification of pathogenic bacteria. When 1000 mL of basal medium is routinely sterilized and cooled to about 55 ° C, the following antibiotics (all active ingredients) are added. Penicillium 50mg, rifampicin 20mg, carbendazim 25mg, pentachloronitrobenzene 25mg, shake well, made into a plate. In the absence of pentachloronitrobenzene, the same amount of benzoate can be used instead. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1820-2006_English be delivered?Answer: Upon your order, we will start to translate SN/T 1820-2006_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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