SN/T 1632.1-2013 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1632.1-2013	639	Add to Cart	3 days	Determination of Enterobacter sakazakii (Cronobacter spp.)from debydrated powdered milk for export. Part 1: Isolation and enumeration	Obsolete
SN/T 1632.1-2005	359	Add to Cart	3 days	Milk powder Sakamoto Qi Enterobacter test methods - Part 1: Separation and counting method	Obsolete

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Basic data

Standard ID: SN/T 1632.1-2013 (SN/T1632.1-2013)
Description (Translated English): Determination of Enterobacter sakazakii (Cronobacter spp.)from debydrated powdered milk for export. Part 1: Isolation and enumeration
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X16
Classification of International Standard: 67.100
Word Count Estimation: 16,156
Older Standard (superseded by this standard): SN/T 1632.1-2005
Quoted Standard: GB/T 19489
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the milk Enterobacter sakazakii (Craunot spp) Isolation and counting methods. This standard applies to milk Enterobacter sakazakii (Craunot spp.) Isolation and counting, other food available for implementation.

SN/T 1632.1-2013: Determination of Enterobacter sakazakii (Cronobacter spp.)from debydrated powdered milk for export. Part 1: Isolation and enumeration

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Enterobacter sakazakii from debydrated powdered milk for export.Part 1 (Cronobacter spp.). Isolation and enumeration People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1632.1-2005 Enterobacter sakazakii in milk powder exports (Craunot Sp) test method Part 1. Separation and counting DeterminationofEnterobactersakazaki (Cronobacterspp.) From Part 1. Isolationandenumeration Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1632 "Enterobacter sakazakii in milk powder exports (Craunot sp) test method" is divided into three parts. --- Part 1. Separation and counting; Part --- Article 2. PCR method; --- Part 3. Fluorescent PCR method. This section SN/T 1632 Part 1. This standard was drafted in accordance with GB/T 1.1-2009 given rules. The partial replacement of SN/T 1632.1-2005 "Enterobacter sakazakii in milk powder test methods - Part 1. Separation and counting." This section compared with the SN/T 1632.1-2005, in addition to editorial changes, the major technical changes are as follows. --- Modify the standard English name; --- Modify the selective enrichment medium and inoculation; --- Remove the separation medium crystal violet neutral red bile dextrose agar (VRBGA); --- Increases will further distinguish the genus Craunot to biochemical identification method thereof. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section drafted by. China Inspection and Quarantine Science Research Institute, Tianjin, People's Republic of China Exit Inspection and Quarantine. The main drafters of this section. Zhaogui Ming, Yang Hairong, Pei, Wang Ping, Zhao Yongsheng, Gao Qi Li, Zhang Haibin, Zhang Xia, Hu Yue. This part of the standard replaces the previous editions are. --- SN/T 1632.1-2005. Enterobacter sakazakii in milk powder exports (Craunot Sp) test method Part 1. Separation and counting

1 Scope

SN/T 1632 provisions of this part of the milk powder Enterobacter sakazakii (Craunot sp) Separation and counting methods. This section applies to milk Enterobacter sakazakii (Craunot sp) separation and counting, other foods may refer to.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety

3 equipment and materials

In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows. 3.1 water bath. to maintain the water temperature 45.5 ℃ ± 0.2 ℃. 3.2 Thermometer. Range 1 ℃ ~ 55 ℃, sub-scale 0.1 ℃. 3.3 Incubator. 35 ℃ ~ 37 ℃ and 24 ℃ ~ 26 ℃. 3.4 straw. 1mL, 5mL and 10mL, sub-scale 0.1mL. 3.5 "L" glass coating bar. Diameter 3mm ~ 4mm, 45mm ~ 55mm may be coated area. 3.6 inoculating loop. 3mm diameter. 3.7 Balance. Range 2kg, a sense of the amount of 0.1g. 3.8 Sterilization of sample preparation equipment. sampling spoon, scissors, can opener. 3.9 Sample dilution bottle. 100mL, 125mL, 160mL, 250mL and 2L sample dilution bottles. 3.10 90mm diameter sterile petri dish. 3.11 Enterobacter sakazakii (Craunot sp) control strains. ATCC29544 or other equivalent strains. 3.12 biochemical identification kits. 3.13 automatic biochemical microbial identification system.

4 media and reagents

4.1 trypticase soy protein Agar (TSA) (see Appendix A, A.1). 4.2 Craunot coli broth (see A.2). 4.3 Craunot coli chromogenic medium (see A.3). 4.4 oxidase test reagent (see A.4). 4.5 L- lysine decarboxylase medium (see A.5). ......

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