SN/T 1632.2-2013 English PDFUS$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1632.2-2013: Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method Status: Valid SN/T 1632.2: Historical versions
Basic dataStandard ID: SN/T 1632.2-2013 (SN/T1632.2-2013)Description (Translated English): Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X16 Classification of International Standard: 67.100 Word Count Estimation: 12,197 Older Standard (superseded by this standard): SN/T 1632.2-2005 Quoted Standard: GB/T 6682; GB/T 27403-2008; SN/T 1632.1 Regulation (derived from): National quality recognition (2013) 569 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the milk Enterobacter sakazakii (Craunot spp) of PCR detection method. This standard applies to milk Enterobacter sakazakii (Craunot spp.) In the rapid qualitative detection of other foods for implementation. SN/T 1632.2-2013: Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. (. Cronobacter spp) Determinetion of Enterobacter sakazakii from dehydrated powdered milk for export.Part 2. PCR method People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1632.2-2005 Enterobacter sakazakii in milk powder exports (Craunot sp) Test methods - Part 2. PCR methods DeterminetionofEnterobactersakazaki (Cronobacterspp.) Fromdehydrated powderedmilkforexport-Part 2. PCRmethod Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordSN/T 1632 "Enterobacter sakazakii in milk powder exports (Craunot sp) test methods" is divided into three parts. --- Part 1. Separation and counting; Part --- Article 2. PCR method; --- Part 3. Fluorescent PCR method. This section SN/T 1632 Part 2. This section drafted in accordance with GB/T 1.1-2009 given rules. The partial replacement of SN/T 1632.2-2005 "Enterobacter sakazakii in milk powder test methods - Part 2. PCR testing methods." This section compared with the SN/T 1632.2-2005, the main technical changes are as follows. --- The standard according to GB/T 1.1-2009 specification text format; --- Modify the standard English name; --- Deleted the definitions, terms and abbreviations; --- Remove the separation medium crystal violet neutral red bile dextrose agar (VRBGA). Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This part by the National Certification and Accreditation Administration of People's Republic of China and focal points. This section was drafted by. People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this section. Zhang Xia, Liu Pei, Zhaogui Ming, Gao Qi Li, Wu winter snows, Zhang Haiying, Qu Peng, Zhao Liangjuan, Zhang Hongwei. This part of the standard replaces the previous editions are. --- SN/T 1632.2-2005. Enterobacter sakazakii in milk powder exports (Craunot sp) Test methods - Part 2. PCR methods1 ScopeSN/T 1632 provisions of this part of the milk powder Enterobacter sakazakii (Craunot sp) of the PCR detection method. This section applies to milk Enterobacter sakazakii (Craunot sp) the rapid qualitative detection of other foods may refer to.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 27403-2008 food quality control laboratory of molecular biology standardized testing SN/T 1632.1 milk Enterobacter sakazakii (Craunot sp) - Part 1. Separation and Counting3 equipment and materials3.1 Balance. Range 2kg, a sense of the amount of 0.1g. 3.2 water bath. to maintain the water temperature 44.5 ℃ ± 0.2 ℃. 3.3 Incubator. 35 ℃ ~ 37 ℃ and 43 ℃ ~ 45 ℃. 3.4 PCR instrument. 3.5 centrifuge. 3.6 UV gel imager. 3.7 electrophoresis. 3.8 pH meter. 3.9 Pipette. 2μL ~ 10μL, 10μL ~ 100μL, 20μL ~ 200μL, 100μL ~ 1000μL.4 media and reagentsTest water should comply with GB/T 6682 stipulated a water. 4.1 Modified lauryl sulfate tryptone broth - vancomycin (mLST-Vm) (see Appendix A, A.1). 4.2 brain heart infusion (BHI) (see A.2). 4.3 nutrient broth (NB) (see A.3). 4.4 Primer SAKAF. 5'-GGGTTGTCTGCGAAAGCGAA-3 '; SAKAR. 5'GTCTTCGTGCTGCGAGTTTG-3'. 4.5 TaqDNA polymerase. 4.6 dNTP. dATP, dTTP, dCTP, dGTP. 4.7 10 × PCR buffer. 4.8 Enterobacter sakazakii control strains. ATCC29544, or equivalent strains. 4.9 DNA extraction kit. bacterial genome DNA extraction kit. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1632.2-2013_English be delivered?Answer: Upon your order, we will start to translate SN/T 1632.2-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1632.2-2013_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1632.2-2013_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version SN/T 1632.2-2013?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1632.2-2013 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |