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SN/T 1405-2004 English PDF

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SN/T 1405-2004: Inspection of 10-hydroxy-α-decenoic acid in bee products for import and export. Capillary electrophoresis method
Status: Obsolete
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1405-2004279 Add to Cart 3 days Inspection of 10-hydroxy-α-decenoic acid in bee products for import and export. Capillary electrophoresis method Obsolete

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Basic data

Standard ID: SN/T 1405-2004 (SN/T1405-2004)
Description (Translated English): Inspection of 10-hydroxy-��-decenoic acid in bee products for import and export. Capillary electrophoresis method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X31
Classification of International Standard: 67.180
Word Count Estimation: 7,767
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the import and export of bee products, 10 carboxy-��-decanoic acid (10-HAD) test sampling, sample preparation and capillary electrophoresis test methods. This standard applies to imports and royal jelly and royal jelly lyophilized powder for 10 carboxy-��-decanoic acid (10-HAD) test.

SN/T 1405-2004: Inspection of 10-hydroxy-α-decenoic acid in bee products for import and export. Capillary electrophoresis method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of 10-hydroxy-α-decenoic acid in bee products for import and export.Capillary electrophoresis method People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard 10-hydroxyl in import and export bee products Method for the determination of α-decenoic acid by capillary electrophoresis Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Hubei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Lin Yanfei, Sun Baoguo, Hu Xiaozhong, Yu Jianxin, Zeng Jing, Wang Peng, Li Jing. This standard is the industry standard for inspection and quarantine issued for the first time. 10-hydroxyl in import and export bee products Method for the determination of α-decenoic acid by capillary electrophoresis

1 Scope

This standard specifies sampling, sample preparation and capillary electrophoresis testing of 10-hydroxy-α-decenoic acid (10-HDA) test in import and export bee products. method. This standard applies to the test of 10-hydroxy-α-decenoic acid (10-HDA) in the import and export royal jelly and royal jelly lyophilized powder.

2 sampling and sample preparation

2.1 Inspection lot The royal jelly is not more than 1000kg for one inspection lot, and the royal jelly lyophilized powder is not more than 250kg for one inspection lot. The same inspection batch should have the same characteristics, such as packaging, marking, origin, specifications, grades, etc. 2.2 Number of samples The number of samples is shown in Table 1. Table 1 is a unit Batch sampling 1 to 50 5 51~100 7 >100 10 2.3 Sampling and sample preparation methods Randomly extract the number of samples according to 2.2, open one by one, and take one can (bag) from each piece as the original sample, mix the sample after mixing Divided into two equal parts, each serving not less than 20g. After the seal is sealed, the mark is marked, one is retained, and the other is sent to the laboratory in time. In sampling and sample preparation The sample must be protected from contamination and changes in the amount of the product during the operation. 2.4 Sample storage The sample was stored frozen below -18 °C.

3 test methods

3.1 Method summary Under acidic conditions, the 10-HDA in the sample is extracted with ethanol. After centrifugation, the supernatant is mixed with the buffer and then subjected to capillary electrophoresis. The instrument was measured and quantified by the internal standard method. 3.2 Reagents and materials Unless otherwise specified, the reagents were of analytical grade and the water was deionized water. 3.2.1 Anhydrous ethanol. excellent grade pure. 3.2.2 Hydrochloric acid solution. 0.03 mol/L aqueous solution. 3.2.3 Internal standard. methylparaben, content 99.0%. 3.2.4 Internal standard solution. Take about 0.65g of dried methylparaben (accurate to 0.0002g), dissolve and shift with absolute ethanol. In a 1000 mL volumetric flask, dilute to the mark with absolute ethanol and shake well. This solution contains about 0.65 mg per ml of internal standard. 3.2.5 Sodium dodecyl sulfate (SDS). 3.2.6 sodium tetraborate decahydrate (sodium tetraborate).
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