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SN/T 4848.2-2017 English PDF

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SN/T 4848.2-2017: Identification of common honey plant species in export honey. Real-time PCR method - Part 2: Canola
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4848.2-2017209 Add to Cart 3 days Identification of common honey plant species in export honey. Real-time PCR method - Part 2: Canola Valid

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YY/T 0681.18   GB/T 317   GB/T 15108   SN/T 4848.3   SN/T 4848.4   SN/T 4848.1   

Basic data

Standard ID: SN/T 4848.2-2017 (SN/T4848.2-2017)
Description (Translated English): Identification of common honey plant species in export honey. Real-time PCR method - Part 2: Canola
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X31
Word Count Estimation: 9,999
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Quoted Standard: GB/T 6682; GB/T 14963-2011; GB/T 27403-2008
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the real-time fluorescent PCR detection method for the distribution of rapeseed components in honey. This standard applies to the qualitative detection of rapeseed components in exported honey. The minimum detection limit (LOD) of the method specified in this standard is 0.1% (mass percent).

SN/T 4848.2-2017: Identification of common honey plant species in export honey. Real-time PCR method - Part 2: Canola


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of common honey plant species in export honey.Real-time PCR method - Part 2. Canola People's Republic of China entry and exit inspection and quarantine industry standards Method for detecting common honey plant components in exported honey Real-time fluorescent PCR - Part 2. Rape Real-timePCRmethod-Part 2. Canola Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4848 "Research method for the detection of common honey plant components in honey is real-time fluorescent PCR" is divided into 8 parts. --- Part 1. Vitex; --- Part 2. Rape; --- Part 3. Artichoke; --- Part 4. Eucalyptus; --- Part 5. Eucalyptus; --- Part 6. Longan; --- Part 7. Lychee and Longan; --- Part 8. Zin Yunying. This part is the second part of SN/T 4848. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Wu Yajun, Chen Ying, Liu Mingchang, Yang Yange, Zhang Jiukai, Han Jianxun, Wang Bin, Li Xiang, Huang Wensheng. Method for detecting common honey plant components in exported honey Real-time fluorescent PCR - Part 2. Rape

1 Scope

This part of SN/T 4848 specifies a real-time fluorescent PCR assay for the distribution of rapeseed components in honey. This section applies to the qualitative detection of rapeseed components in exported honey. The minimum detection limit (LOD) for the method specified in this section is 0.1% (% by mass).

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and experimental methods GB 14963-2011 National Food Safety Standard Honey GB/T 27403-2018 Laboratory Quality Control Specification Food Molecular Biology Testing

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Honey honey The bee collects the nectar, secretions or honeydew of the plant, and mixes it with its own secretions, and then fully brews the natural sweet substance. 3.2 Canola canola Brassica napus (Brassicacampestris L.), also known as oil cabbage, bitter herbs, cruciferae, Brassica, is a Brassicaceae cabbage variety. 3.3 Real-time fluorescent PCR realtimePCR Adding a fluorophore to the PCR reaction system and monitoring the entire PCR amplification process in real time using the accumulation of fluorescent signals to achieve the initial Quantitative and qualitative analysis of the template. 3.4 Ct value cyclethreshold The number of cycles experienced by the fluorescent signal in each reaction tube when it reaches the set domain value.

4 Abbreviations

The following abbreviations apply to this document. DNA. deoxyribonuleic acid PCR. polymerase chain reaction (polymerase chain reaction) dNTP. deoxyribonuleosidetriphosphate
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