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Methods for the quarantine and identification of Xanthomonas axonopodis pv. vasculorum (Cobb 1894) Vauterin, Hoste, Kerster and Swings 1995
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Basic data
| Standard ID | SN/T 1400-2004 (SN/T1400-2004) |
| Description (Translated English) | Methods for the quarantine and identification of Xanthomonas axonopodis pv. vasculorum (Cobb 1894) Vauterin, Hoste, Kerster and Swings 1995 |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 65.020 |
| Word Count Estimation | 8,823 |
| Date of Issue | 2004-06-01 |
| Date of Implementation | 2004-12-01 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China |
| Summary | This standard specifies the entry in the sugar cane plant quarantine and quarantine pathogen identification methods. This standard applies to entry cane cane, cane sugar gum leaves quarantine and identification of bacteria. |
SN/T 1400-2004: Methods for the quarantine and identification of Xanthomonas axonopodis pv. vasculorum (Cobb 1894) Vauterin, Hoste, Kerster and Swings 1995
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Methods for the quarantine and identification of Xanthomonas axonopodis pv.vasculorum (Cobb 1894) Vauterin, Hoste, Kerster and Swings.1995
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Method for quarantine and identification of sugarcane gum
Released on June.2004
Implementation of.2004-12-01
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Zhang Junchao, Huang Shengguang, Ye Shenghua, Wen Xin.
This standard is the industry standard for inspection and quarantine issued for the first time.
Method for quarantine and identification of sugarcane gum
1 Scope
This standard stipulates the quarantine and identification methods of sugarcane flow pathogens in the entry plant quarantine.
This standard is applicable to the quarantine and identification of sugar cane bacillus in sugarcane stems and sugarcane leaves.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 4789.28-1994 Food hygiene microbiological examination Staining method, medium and reagents
3 Principle
Sugarcane genus is a prokaryotic organism (Procaryotes), a thin-walled bacterium (Gracilicutes), Pseudomonas
Obvious symptoms, pathogens have unique culture traits and physiological and biochemical characteristics. Symptoms of disease, morphology of pathogens, culture of pathogens, physiology
Biochemical traits and pathogenicity determination are the basis for the identification of sugarcane gum.
4 instruments and appliances
4.1 Instrument
Biological microscope, biological incubator, etc.
4.2 Appliances
Autoclave, ordinary balance, ultra-clean workbench, alcohol lamp, petri dish, test tube, glass slide, coverslip, inoculation needle or sewing needle, inoculation
Rings, pipettes, knives, scissors, tweezers, glass rods, etc.
5 media and reagents
5.1 YPGA medium formula. yeast extract 5g/L, glucose 20g/L, peptone 10g/L, agar 15g/L, pH 7.2.
5.2 YDC medium formula. yeast extract 10g/L, D-glucose 20g/L, calcium carbonate 20g/L, agar 15g/L, pH 7.2.
5.3 GYCA medium formula. yeast extract 5g/L, glucose 5g/L, calcium carbonate 40g/L, agar 15g/L.
5.4 Gelatin, mercuric acid solution (12g of mercury chloride, 80mL of distilled water, 16mL of concentrated hydrochloric acid).
5.5 Potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium sulfate, sodium nitrate.
5.6 peptone, dipotassium hydrogen phosphate, esculin, ferric citrate.
5.7 Beef extract, sodium chloride, agar, soluble starch.
5.8 Acid-producing gas production and use of carbon source, nitrogen source Test reagent. xylose, arabinose, fructose, glucose, mannose, galactose, sucrose, fiber
Disaccharide, glycerin, maltose, rhamnose, inulin, salicin, mannitol, sorbitol, galactitol, adonitol, α-methyl-D-glucoside
Liquid, acetate, propionate, citrate, malate, succinate, lactate, benzoate, oxalate, tartrate, gluconate,
Asparagine.
...
