SN/T 1274-2020 English PDF

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SN/T 1274-2020: (Quarantine and Identification Methods of Phaseolus vulgaris)
Status: Valid

SN/T 1274: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1274-2020	259	Add to Cart	3 days	(Quarantine and Identification Methods of Phaseolus vulgaris)	Valid
SN/T 1274-2003	319	Add to Cart	3 days	Inspection and Identification of Acanthoscelides obtectus (Say)	Obsolete

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Basic data

Standard ID: SN/T 1274-2020 (SN/T1274-2020)
Description (Translated English): (Quarantine and Identification Methods of Phaseolus vulgaris)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 12,176
Date of Issue: 2020-12-30
Date of Implementation: 2021-07-01
Older Standard (superseded by this standard): SN/T 1274-2003
Regulation (derived from): General Administration of Customs Announcement No. 136 [2020]
Issuing agency(ies): General Administration of Customs

SN/T 1274-2020: (Quarantine and Identification Methods of Phaseolus vulgaris)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Acanthoscelides obtectus (Say) The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards Replace SN/T 1274-2003 Quarantine and Identification Methods of Phaseolus vulgaris 2020-12-30 release 2021-07-01 implementation Issued by the General Administration of Customs of the People's Republic of China

Foreword

This document was drafted in accordance with the rules given in GB/T 1.1-2020. This document replaces SN/T 1274-2003 "Methods of Quarantine and Identification of Phaseolus vulgaris". Compared with SN/T 1274-2003, the main technical changes of this document are as follows except for editorial changes. --The identification characteristics of Leguminosae and Tridentate genus have been added; --Added basic information content; -Added Appendix A, Appendix B and Appendix C; --Delete the table of contents; - Delete some instruments and reagents; - Deleted on-site and indoor inspections. This document was proposed and managed by the General Administration of Customs of the People's Republic of China. Drafting organizations of this document. Chinese Academy of Inspection and Quarantine, Changchun Customs of the People’s Republic of China, Guangzhou of the People’s Republic of China customs. Drafters of this document. Zhang Junhua, Wei Chunyan, Qi Hushan, Chen Naizhong, Yu Yanxue, Zhou Ping. The previous versions of the documents replaced by this document are as follows. - SN/T 1274-2003. Quarantine and Identification Methods of Phaseolus vulgaris

1 Scope

This document stipulates the basic information, identification methods, equipment and methods of Acanthoscelides obtectus (Say) in plant quarantine. Reagents, testing, laboratory identification, as well as the method of result determination and specimen preservation. This document is applicable to the quarantine and identification of Phaseolus vulgaris in plant quarantine.

2 Normative references

There are no normative references in this document.

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Larval lower lip larval labium The lower lip of the bean weevil larva is a sheet-like structure at the bottom of the mouthparts, which is slightly triangular, concave on both sides, and the posterior edge is convex. Asian chin composition. The chin has a shield-shaped bone plate called the lower lip plate. The front of the lower lip plate is divided into two parts. The sub-chin is long, and there are some sub-chin bones. The type is not obvious, and some types are broken in the middle, and some types of submental bone fragments are intact. 3.2 Male antagonist side process arameres It is part of the male genitalia. Located on the back of the penis, it is made up of two leaves and is spoon-shaped with enlarged ends. In different classes In clusters, some of the two male base scoliosis are separated at the base, some are partially healed, and some are almost completely healed. 3.3 Male phallus, endophallus and its sclerites phallus, endophallus and its sclerites The penis is a component of the male external genitalia. It is located on the ventral surface of the male base side. It is rod-shaped, and the base is enlarged and sac-shaped (called cyst Area), the end is petal-shaped (called the outer penis petal). The penis is divided into outer penis and inner penis. The outer phallus is the outer part of the phallus, the inner phallus The stem is membranous, lining the outer penis. There are a large number of ossified spines on the inner phallus, arranged in a certain pattern, with species specificity, for One of the important basis for the identification of leguminous insect species.

4 Basic information

The basic information of Phaseolus vulgaris is as follows.

5 Identification method

Bean weevil that harms edible beans, mainly in the genus Bruchus, Callosobruchus, and Tridentia Acanthoscelides, Caryedon and Zabrotes, which have the same host as the common bean The genus Leptophyllum, Leptophyllum genus and Leptospira genus, and the genus of Leptophyllum is only one species in the genus that has important economic significance. Three tooth beans Based on the comparison of the morphology between the genus and other genus and the inter-species of the genus Tridentia, the identification characteristics of the genus Bean Weevil are determined by stereo visualization. According to microscopic observation, the external morphological characteristics of adults are the main basis for the identification of this standard. When the larvae are obtained, they are raised until the adults, and then Do further identification.

6 Equipment and reagents

6.1 Equipment Stereo microscope, light incubator, forceps, dissecting scissors, dissecting needle, petri dish, absorbent paper, small brush, finger tube. 6.2 Reagents 10% sodium hydroxide (NaOH), absolute ethanol, 75% ethanol, larval preservation solution (75% ethanol. glycerol=100.0.5~1).

7 Detection

All small beans carried by the ship’s food warehouses and passengers and internationally mailed or delivered as samples shall be inspected. For goods The appearance of the packaging, the bedding materials, the sides of the car, ship, container, the sides, corners, and gaps of the bag, use the naked eye to see if there are any beans Like live adults or dead adults. Check whether there are larvae bore holes on the beans on the sieve, and whether there are "small windows" or emergence holes.

8 Laboratory identification

8.1 Feeding When the insects are larvae, they need to be raised as adults. Put the host with insects in the insect breeding box, keep the temperature at 25℃~30℃, relatively The humidity is 70%~90%. The gauze gate of the insect breeding box prevents adult insects from escaping. 8.2 Distinguishing characteristics of Leguminosae adults The head stretches like a short nose, and it closely adheres to the front chest and mid-thoracic abdomen in a static state; the concave edge in front of the compound eye, most types of concave edge are extremely deep It is horseshoe-shaped, and a few species are dimpled; the antennae are 11 knots, laterally flat, and the rest of the knots except the base are mostly expanded into serrated or comb-toothed, Born at the opening of the concave edge of the compound eye. The pronotum is conical, trapezoidal or semicircular. Each elytra has 9 to 10 longitudinal patterns, and the space between the rows Engraved dots are scattered within the lines. The buttocks are large and exposed. In some groups, in addition to the buttocks, there are 1 to 2 sections of the dorsal plate exposed. Abdomen visible in the abdomen Board 5 sections. The forefoot and midfoot have thin leg sections, and the hind leg sections are thick and flat. There are 1 to 2 longitudinal ridges on the ventral surface. The ridges usually have teeth. Or dentition. 8.3 The distinguishing characteristics of Tridentia The prothoracic dorsal plate has no lateral ridges; the feet are thick, and the hind leg segments only have teeth on the inner ridge, generally with 1 large tooth and 2 to 3 small teeth; The tibia of the hind foot has no distance, usually with sharp protrusions and spines; only the buttocks of the abdomen are not covered by the elytra (see Appendix B). 8.4 Identification characteristics of Phaseolus vulgaris 8.4.1 Adult The body length is 2.0 mm~4.0 mm. The head, chest and elytra are black, densely covered with yellow hair, and dark gray on the back, similar to common bean elephants. There is a clear difference. The abdomen and buttocks are orange-red, densely covered with white hairs, mixed with yellow hairs. The head is long and wide, densely engraved, and the forehead line Smooth without markings. Antennae 11 sections, base 4 sections and 11 sections are orange, the rest are black, base 4 sections are filamentous, sections 5~10 The knots are serrated, the end is peach-shaped, and the ends are tapered. The pronotum is conical. There are 3 teeth at the proximal end of the ventral surface of the hind leg, one is long The big pointed tooth is followed by 2 small teeth, and the length of the large tooth is about twice the length of the 2 small teeth. After anatomy, the male genitalia has a longer penis, The end of the outer penis valve is blunt, concave on both sides, the tips of the leaves on both sides are enlarged, and the front end of the inner penis has two longitudinal micro-thorn areas, and the color of the sac area is dark (see Appendix C). 8.4.2 Egg White, translucent, oval, round at both ends, and one end is wider than the other. The eggs do not adhere to the surface of the beans. Egg width 0.264 mm± 0.046 mm, the egg length is 0.660 mm±0.089 mm, and the ratio of width to length is 0.40 on average. 8.4.3 Larva The first instar larva is about 0.8 mm long and 0.3 mm wide. The middle chest and back chest are widest, tapering towards the abdomen. 1 on each side of the head The small eyes are located between the upper jaw and the antennae. 1 section of tentacles. The anterior chest shield is in the shape of "X" or "H" with denticles on it. 8th and 9th belly Nodal dorsal plate has an oval ossification plate. The foot consists of 2 sections. The body length of the mature larva is 2.4 mm~3.5 mm, and the width is 1.6 mm~2.3 mm. The body is stout, curved in a "C" shape; the feet are degenerate. The upper lip has 10 bristles, of which 8 are located near the outer edge, arranged in an arc, and the rest 2 The roots are located on both sides of the base. There is no anterior chest shield, and no ossification plate on the 8th and 9th abdominal segment dorsal plates. 8.4.4 Pupa 3.2 mm~5.0 mm long, oval, light yellow.

9 Results judgment

Taking 8.4.1 as the main basis, 8.4.2, 8.4.3, 8.4.4 and appendices can be used as reference, and those that conform to 8.4.1 are judged as common bean weeds. 10 Specimen preservation Keep the kidney bean weevil and the important damage specimens properly. The larvae and pupae should be preserved in the larvae preservation solution. For molecular testing, use the larvae preservation solution. Soaked in absolute ethanol, the adult worms are made into needle specimens. Record the name, source, interception time, location, personnel and other relevant information of the pest, The general shelf life is at least 6 months.

Appendix A

(Informative) Basic Information of Bean Elephant A.1 Geographical distribution Asia. North Korea, Japan, Myanmar, Vietnam, Thailand, Afghanistan, Turkey, Malaysia, India, Israel, Iraq Croatia, Georgia, Kazakhstan, Tajikistan; Europe. Russia, Poland, Hungary, Germany, Austria, Switzerland, Netherlands, Belgium, United Kingdom, France, Spain Portugal, Italy, the former Yugoslavia, Romania, Albania, Bulgaria, Czech Republic, Slovakia, Luxemburg Fort, Greece; Africa. Nigeria, Ethiopia, Kenya, Uganda, Burundi, Congo, Angola, Togo, Egypt, Malaysia Ravi, Mauritius, Rwanda, Zambia, Tanzania, Senegal, Zimbabwe, Morocco, Lesotho, South Africa; Oceania. Australia, New Zealand, Papua New Guinea; North America. Canada, the United States, Mexico, Honduras, Belize, Costa Rica, Cuba, Dominica, Guade Rope (France), El Salvador, Guatemala, Nicaragua; South America. Argentina, Bolivia, Brazil, Colombia, Guyana, Paraguay, Peru, Venezuela. A.2 Host plant A.3 Biological characteristics At an average temperature above 15°C, overwintering larvae begin to pupate and emerge as adults. The adults can mate within a few minutes or hours after they emerge. It occurs 7 generations per year under natural indoor conditions in Wanding. The spawning period is 5 days in summer and 39 days in winter. Early summer adults can fly out of the warehouse, Feed nectar in the field. The eggs lay in the cracks of the dry pods, and the eggs lay between the beans in the warehouse. Adults that feed in the field do not feed in the barn The adults lay more eggs. The eggs hatch into larvae a few days later, biting through the seed coat and entering the seed. Old mature larvae eat away from the inside of the seed to the outer skin, and Moth an eclosion hole. After emergence, the adult larva stays still in the bean for 1 to 3 d, and crawls out with the emergence hole on the top of the head and forefoot. At 75% humidity, At 26℃, the egg stage is 8.4 days, the larval stage is 18.6 days, and the pupal stage is 9 days. A.4 Means of transmission Phaseolus vulgaris is a dangerous pest that harms many types of Phaseolus vulgaris and other edible beans, and causes serious harm to stored beans. Eggs, larvae, Both pupae and adults can be carried. Phaseolus vulgaris is easy to spread widely with the host, mainly through trade, introduction and transportation with the help of infested beans. Transmission tools, etc. A.5 Hazard characteristics The eggs laid by adults have no sticky substance and cannot adhere to the seed coat, but are scattered among the bean grains; the larvae hatched from the eggs crawl around To find a suitable injection point. After boring into the seed, a circular boring hole with a diameter of 0.13 mm~0.24 mm is left on the surface of the seed. The larvae are 4 instars and all eat in the seeds. Before pupation, the mature larva runs under the seed coat to make a small round translucent window. And bite around the small window into a circular feather hole cover. When adults emerge, open the emergence hole cover to leave the bean, leaving a round feather 化孔.

Appendix B

(Informative) Key to the subfamily Bruchinae 1 The pronotum and dorsal plate are horizontal, the lateral edge is recessed near the middle, and there is an dentition in the front; there is an dentition on the outer edge of the ventral surface of the hind leg; There is an odontoid or lamellar process at the end of the tibia of the foot; ♂The external genital sac is only densely covered with microhairs, without ossification plate... ......

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