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Basic dataStandard ID: SN/T 1264-2020 (SN/T1264-2020)Description (Translated English): (Quarantine and identification methods for boll elephants in Mexico) Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 12,173 Date of Issue: 2020-12-30 Date of Implementation: 2021-07-01 Older Standard (superseded by this standard): SN/T 1264-2003 Regulation (derived from): General Administration of Customs Announcement No. 136 [2020] Issuing agency(ies): General Administration of Customs SN/T 1264-2020: (Quarantine and identification methods for boll elephants in Mexico)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Anthonomus grandis Boheman The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards Replace SN/T 1264-2003 Quarantine and identification methods for boll elephants in Mexico 2020-12-30 release 2021-07-01 implementation Issued by the General Administration of Customs of the People's Republic of China ForewordThis document is drafted in accordance with GB/T 1.1-2020. This document replaces SN/T 1264-2003 "Mexican Boll Elephant Identification Method". Compared with SN/T 1264-2003, the main technical changes of this document are as follows except for editorial changes. -Added detailed identification feature descriptions of subfamily, morphological feature differences and feature maps of similar species; -Deleted the introduction, some common terms and definitions, some instruments and reagents, sample preparation and storage. This document was proposed and managed by the General Administration of Customs of the People's Republic of China. Drafting organizations of this document. Chinese Academy of Inspection and Quarantine, Changchun Customs, People’s Republic of China, Xi’an, People’s Republic of China customs. The main drafters of this document. Zhang Junhua, Wei Chunyan, Liu Yuanye, Chen Naizhong, Yu Yanxue, Zhou Ping. The previous versions of the documents replaced by this document are as follows. Quarantine and identification methods for boll elephants in Mexico1 ScopeThis document stipulates the basic information, identification methods, methods of identification of the Mexican boll elephant Anthonomus grandis Boheman in phytosanitary conditions. Methods of equipment and reagents, testing, experimental identification, result determination and specimen retention. This document is applicable to the quarantine and identification of Mexican boll elephants in plant quarantine.2 Normative referencesThere are no normative references in this document.3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Beak rostrum For any extended mouthparts or front extensions of the head, the beak of a weevil is formed by extending the forehead forward. 3.2 Antennal groove scrobe The antennal groove is located in the beak, it is the groove that accommodates the antennal shank. 3.3 Stria The row pattern is the longitudinally arranged grooves on the back of the elytra, including rows of engraved dots. Under normal conditions, an elytra has 10 lines, Starting from the elytra seam, mark the lines 1~10. 3.4 Interval between lines The area between every two rows of elytra is the inter-row. Under normal circumstances, an elytra has 11 rows, starting from the elytra slit, Mark the lines as 1~11. 3.5 Pygidium Expose the somites outside the elytra, you can see the dorsal plate of the abdomen. 3.6 Spermatheca The spermatheca is a part of the female genitalia, a cyst-like structure used to receive sperm during mating.4 Basic informationThe basic information of the Mexican boll elephant is as follows. Scientific name. Anthonomus grandis Boheman English name. Cotton boll weevil Mexican boll weevil belongs to Coleoptera, Curculionidae, Curculioninae, Anthonomus. The similar species is Anthonomus quadrigibbus Say, both of which have important quarantine significance. See Appendix A for other relevant information about the Mexican boll elephant.5 Identification methodObserved with a stereo microscope, the external morphological characteristics of adults are the main basis for the identification of this standard. When the larvae are obtained, feed After adulthood, further identification will be done.6 Equipment and reagents6.1 Equipment Stereo microscope, light incubator, forceps, dissecting scissors, dissecting needle, sealing bag, petri dish, absorbent paper, small brush, Finger tube. 6.2 Reagents 10% sodium hydroxide (NaOH), absolute ethanol, 75% ethanol, larval preservation solution (75% ethanol. glycerol=100.0.5~1).7 DetectionSeed cotton and cottonseed carry the risk of spreading this insect. At the quarantine site, imported cottonseeds and seed cotton should be strictly inspected. For the epidemic, the quantity must be strictly controlled, and the shipper must issue an official fumigation certificate to ensure that no live insects exist. In the import inspection, if a livelihood is found Insects should be exterminated. Although lint is less likely to carry insects, it must be tested to prevent it from being mixed with insect-carrying cottonseeds. test If cottonseeds are found in the middle, they must also be fumigated; check the inside and outside, the upper and lower walls, the corners of the gaps, And packaging, bedding, residues and other places where pests may hide; goods stored in warehouses or other places should be checked Whether there are traces of pests and pest activities on the surface of the stack, the corners of the stack, the outside of the package, the corners of the bag, and the surrounding environment. If larvae are found, send them together The master puts it in the sealed bag together. Place it in a petri dish, sealed bag with a label or number, and record the time, location, host, type of goods, Detailed information from country or region, inspector, etc.8 Laboratory identification8.1 Feeding When the insects are larvae, they need to be raised as adults. Put the insect-carrying host in the insect-raising box, keep the temperature at 25℃~30℃, relatively wet Degree 70%~90%. The gauze gate of the insect breeding box prevents adult insects from escaping. 8.2 The distinguishing characteristics of adults of the subfamily Weevil They are small to medium-sized, mostly sturdy, with the front and back ends of the shoulders shrunk into a diamond shape, and the body is covered with scales of different colors and shapes. The antennae are slender, mostly located in the middle of the beak, inserted in the middle of the antennal groove, and the stalk usually does not exceed the front edge of the eye, and is rod-ovate. The eyes are usually large, Facing forward, round or long oval. Forehead width is smaller than eye width. The beak is usually slender, curved and cylindrical. Some groups of females have much longer beaks than males, Some are longer than body length. The beak surface has no bristles or only sparse bristles, unevenly distributed. The base of the antennal groove is located on both sides of the beak, from the back Invisible. The head is small, hemispherical, the front chin is not enlarged to cover the lower jaw, and the lower jaw is visible on all sides of the chin. The upper jaw is located on the back of the beak, Up and down activities. The posterior side panels of the mid-chest do not rise and are not visible from the back. The tibia has no spines, and the top comb-shaped bristles are transversely perpendicular to the tibial axis. Larvae are parasitic in various environments, such as living plant tissues including stems, leaves, flower buds, fruits and seeds, the length of the beak and the size of the damaged fruit Size related. 8.3 Distinguishing characteristics of adults of the genus Weevil The jaw moves vertically; the beak is usually slender and long; the eyes are on the side, not protruding from the surface of the head, and the ventral surface of the head is separated from the eyes; no prothorax Ventral groove; antennae cords 7 segments; lateral edge of elytra is straight or slightly curved at base segment of hindfoot; elytra is obtuse at base posterior, not facing The front protrudes below the corner of the pronotum; the back side panel has a small notch on both sides of the end of the dorsal corner; the top of the pronotum is slightly concave or not, The depression is at least as long as the base segment; the tibia is slender; the base of the tarsal claw is separated, with teeth (with base protrusions or teeth). This genus has important quarantine The two species of significance are the Mexican boll elephant Anthonomus grandis Boheman and its similar species, the apple flower elephant Anthonomus Quadrigibbus Say, see Appendix B for the main morphological differences between the two, and Appendix C for the morphological feature diagram. 8.4 Identification characteristics of the Mexican cotton boll elephant 8.4.1 Adult 8.4.1.1 Female. body length 4.5 mm, width 2.2 mm, oblong, reddish brown to dark red, covered with rough spots and hairs. head Conical shape, with fairly protruding eyes. The beak is slender, slightly contracted from both ends to the middle, the antennae are embedded farther from the end than the male, and the base of the beak is sparse Fluffy. The antenna has 7 knots, the cable knot 2 is longer than the cable knot 3, the cable knots 3~7 are equal in length, the antenna rod is 3 knots, the cable and the rod joints have the same color. Front chest The back panel is 1.5 times wider than the length, the widest point is in the middle, and the sides are almost straight from the base to the middle, and the back angle is right-angled. The front end is not narrowed and round. Back The surface is quite raised, densely engraved. The elytra is long oval, the base is slightly wider than the pronotum, and gradually widens backwards, the base 2/3 is almost parallel, The ends gradually shrink into a round shape. The elytra rows are engraved deep and close to each other, the rows are slightly raised, and the widths between odd and even rows are equal, but Interline 4 There is polymorphism at the base, which is normal or narrow or discontinuous. There are no obvious spots on the hind wings. The buttocks are exposed. The forefoot leg sections are very thick and rod-shaped, There are two thick teeth, the inner teeth are long and thick, the outer teeth are sharp triangles, and the bases of the two teeth are conjoined. The middle and hind legs are not as good as the front legs The joint is thick and has only 1 tooth. The tibia is thick, with two curved waves on the inside of the front end, and straight at the back end. The tarsus is well developed, the claws are free, and the claws of the forefoot tarsus have The phenomenon of hermaphroditism, the inside of female claws have slender and sharp teeth, the length of which is almost equal to claws. The hairs on the abdomen of the body are dense. 8.4.1.2 Male. Body length is 5.0 mm, width is 3.0 mm, light body color. The beak is slightly shorter and thicker than that of the female, and the two sides of the beak are nearly parallel. The engraved dots are large, and the antennae is embedded at 1/3 from the end to the eye, which is closer to the end of the beak than the female. The teeth on the inside of the claws are more The thickness is thick, and the sharpness of the end is not obvious. 8.4.2 Egg The egg is 0.8 mm long and 0.5 mm wide, white and oval. 8.4.3 Larva The body length of the mature larva is slightly larger than 8 mm, white, footless, C-shaped body, covered with a few bristles, light yellow-brown head, and airy abdomen The hole is two-hole shape. 8.4.4 Pupa Naked pupa, milky white.9 Results judgmentTaking 8.4.1 as the main basis, 8.4.2, 8.4.3 and 8.4.4 can be used as reference, and the judgement that meets 8.4.1 is the Mexican boll elephant. 10 Specimen preservation The Mexican boll elephant and important damage specimens should be properly preserved. The larvae and pupae should be preserved in the larval preservation solution. For testing, it is soaked in absolute ethanol, and the adult worms are made into needle-inserted specimens. Record the name, source, interception time, location, personnel, etc. of the pest Related information, the general storage period is at least 6 months.Appendix A(Informative) Basic information of the Mexican boll elephant A.1 Geographical distribution Europe. Greece, Italy, Spain. Africa. Benin, South Africa. Central America and the Caribbean. Belize, Costa Rica, Cuba, Dominican Republic, El Salvador, Guatema La, Haiti, Honduras, Martinique, Nicaragua, Saint Kitts and Nevis. South America. Argentina, Brazil (Alagos, Bahia, Ceara, Maranhao, Mato Grosso do Sul, Minasgui Las, Paraíba, Parana, Pernambuco, Piauí, Rio Grande do Norte, Sao Paulo) Colombia, Ecuador, Paraguay, Venezuela. North America. United States (Alabama, Arkansas, Florida, Georgia, Louisiana, Mississippi, North Carolina California, South Carolina, Tennessee, Virginia, Arizona, Oklahoma, Texas, Missouri), Mexico (Wei La Cruz, Nuevo Leon, Guerrero, Chihuahua, Sonora, Tamaulipas, Southern Baja California, Chiapas, Duran Go, Jacosco, Morelos, Nayarit, Sinaloa, San Luis Potosí), Guatemala, El Salvador, Hong Durres, Nicaragua, Costa Rica, Cuba, Haiti, Dominica. Asia. India (Western). A.2 Host plant Mexican cotton... ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1264-2020_English be delivered?Answer: Upon your order, we will start to translate SN/T 1264-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1264-2020_English with my colleagues?Answer: Yes. 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