SN/T 1203-2010 English PDFSN/T 1203: Historical versions
Basic dataStandard ID: SN/T 1203-2010 (SN/T1203-2010)Description (Translated English): To edible fats transit qualitative detection method for real-time PCR gene plant ingredients Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X14 Classification of International Standard: 67.200 Word Count Estimation: 9,93 Date of Issue: 2010-11-01 Date of Implementation: 2011-05-01 Older Standard (superseded by this standard): SN/T 1203-2003 Quoted Standard: GB/T 6682; GB 27025; GB/T 19495.2; SN/T 1204 Regulation (derived from): National Quality Inspection (2010) 582 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the transgenic plant ingredients edible fats real-time PCR qualitative detection method. This standard applies to edible fats transgenic plant ingredients qualitative real-time fluorescence PCR assay. This standard does not apply to animal fats transgenic plant ingredients qualitative real-time fluorescence PCR assay. SN/T 1203-2010: To edible fats transit qualitative detection method for real-time PCR gene plant ingredients---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. To edible fats transit qualitative detection method for real-time PCR gene plant ingredients People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1203-2003 Edible oils and fats transgenic plant components Real-time PCR for qualitative detection method Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1203-2003 "edible oil plant genetic component Qualitative PCR Detection of transit." This standard compared with SN/T 1203-2003, the main technical changes are as follows. --- Re-regulate the scope of use of vegetable oil; --- Redefined the nucleic acid extraction method of edible oils and fats; --- Replace conventional PCR detection PCR and fluorescent PCR (incorporation assay) for the real-time fluorescence PCR; --- Adjustment of the qualitative PCR primers, probes sequences; --- Redefined judgment based on test results. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong Exit Inspection and Quarantine, Beijing Wanger Biotechnology Co., Ltd. The main drafters of this standard. Li Danning, east Wei, Xie force, Zhong Yuqing, Zhongguo Qiang, Cai Ying, Wan Yu Ping, Dong Jie, Deng Hong Ling, Ma Jun, Zhang Jun, Chen source tree, Xie Pei heart. This standard replaces the standards previously issued as follows. --- SN/T 1203-2003. Edible oils and fats transgenic plant components Real-time PCR for qualitative detection method1 ScopeThis standard specifies the composition of plant gene real-time PCR qualitative detection method of edible oil transit. This standard applies to edible oils transgenic plant components in real-time fluorescence Qualitative PCR Detection. This standard does not apply to animal fats transgenic plant components in real-time fluorescence Qualitative PCR Detection.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6682 analytical laboratory use specifications and test methods GB/T 27025 testing and calibration laboratories General requirements GB/T 19495.2 technical requirements for GMO testing laboratories SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 DNAextractionandpurification DNA extraction and purification of DNA is released from the various components of the test sample, followed by removal of the purified PCR DNA reaction inhibitor. 3.1.2 Exogenous gene exogenousgene Use of other bio-genetic bioengineering technology transferred, so that the performance of the new biological species biological traits. 3.2 Acronyms The following abbreviations apply to this document. EPSPS (5-enolpyruvylshkimate-3-phosphatesynthasegene). 5- shikimate-3-phosphate synthase gene FAM (6-carboxyfluorescein). 6- carboxyfluorescein NOS (terminatorofnopalinesynthasegene). nopaline synthase gene terminator TAMRA (carboxy-tetramethyl-rhodamine). carboxy tetramethylrhodamine tRNALeu. chloroplast RNA leucine transporter gene Principle 4 This standard relates to edible oil is oil crops processing products, usually at room temperature, an oily liquid, oil pressing, extraction process , Part of the nucleic acid and other polar group with the destruction of oil crops branch cells into the oil. Using appropriate nucleic acid extraction technology, access to suitable ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1203-2010_English be delivered?Answer: Upon your order, we will start to translate SN/T 1203-2010_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version SN/T 1203-2010?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1203-2010 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
