SN/T 1135.5-2007 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1135.5-2007	359	Add to Cart	3 days	Identification of Clavibacter michiganensis subsp.sepedonicus	Obsolete

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NY/T 2288   SN/T 1204   NY 39   SN/T 1135.8   SN/T 1135.3   SN/T 1135.2   

Basic data

Standard ID: SN/T 1135.5-2007 (SN/T1135.5-2007)
Description (Translated English): Identification of Clavibacter michiganensis subsp.sepedonicus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020
Word Count Estimation: 9,920
Date of Issue: 2007-12-24
Date of Implementation: 2008-07-01
Regulation (derived from): Industry standard filing Notice 2008 No. 2 (No. 98 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the potato ring rot pathogen quarantine and identification. This standard applies to all entry and exit of merchandise with potato and potato quarantine and identification.

SN/T 1135.5-2007: Identification of Clavibacter michiganensis subsp.sepedonicus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Clavibacter michiganensis subsp.sepedonicus Exit inspection and quarantine industry standard book People's Republic of China Potato Ring Rot quarantine and identification methods Posted 2007-12-24 2008-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1135 is divided into five parts. --- Potato quarantine and identification of cancer disease; --- Potato yellow dwarf virus quarantine and identification methods; --- Potato mop top virus quarantine and identification methods; --- Potato smut quarantine and identification of bacteria; --- Potato Ring Rot quarantine and identification methods. This section is SN/T 1135 Part 5. This section Appendix B, Appendix C are normative appendices, Appendix A is an information appendix. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section drafted by. China Inspection and Quarantine Science Research Institute, Beijing People's Republic of China Exit Inspection and Quarantine. The main drafters of this section. Zhao Wenjun, Chen Yun, Liang Miao, Zhang Yue, Zhu Shuifang, Weimei Sheng. The first part of the Department of Inspection and Quarantine issued by industry standards. Potato Ring Rot quarantine and identification methods

1 Scope

SN/T 1135 provisions of this part of the potato ring rot pathogen quarantine and identification methods. This section applies to all entry and exit Seed Potato and merchandise using potato quarantine and identification of.

2 Terms and definitions

The following terms and definitions apply to SN/T 1135 to the present section. 2.1 Immunosorbent assay double antibody sandwich enzyme-linked (ELISA plate) coated with bacteria-specific antibodies on the solid phase support, after the sample is added, and then an enzyme-labeled antibody for bacteria Identification A serological detection of bacteria by detecting the presence or absence of the final enzymatic reactions. 2.2 PCR In thermostable DNA polymerase and a pair of primers (tested with target nucleic acid molecule sequences homologous DNA fragments) at high temperature (DNA points Sub-denatured) and low temperature (primers and the target nucleic acid molecule complex nature and thermostable DNA polymerase extension) alternating cycles of amplification of the target nucleic acid test Child approach. Principle 3

4 apparatus and appliances

4.1 Equipment PCR instrument, clean bench, sterilizer, ice maker, nucleic acid protein analyzer, high-speed refrigerated centrifuge, small desktop centrifuge, ultra-low temperature Refrigerator, conventional refrigerator, Vortex, micro-injector, electrophoresis, gel imaging system, microplate reader. 4.2 Reagents Unless otherwise specified, all reagents were of analytical grade. PCR buffer, magnesium chloride (MgCl2), dNTPs (dATP, dTTP, dCTP, dGTP), T Rao q DNA polymerase, primers and probes, beef extract, yeast extract, dipotassium hydrogen phosphate (K2HPO4), potassium dihydrogen phosphate (KH2PO4), magnesium sulfate (MgSO4), dextrose, agar, ELISA detection reagent in Appendix B. Identify bacteria 5 5.1 Symptom Check Take a sample amount of 1% to 5% (if less may be appropriate to increase the proportion of the sample) of tubers to be checked. The tubers cross check ring rot pathogen infection Are symptoms, squeeze tubers, check the vascular tissue maceration. The incidence of vascular yellow light, was not continuous color dots; severe entire vascular bundle Ring color. Bacteria can also be against tuber parenchyma around vascular bundle, a ring rot can cause severe skin and pith tissue isolation, Gently squeeze cheese-like tubers gush of pillars from the vascular bundles. Symptoms tuber surface in mild hazard is not obvious, with the development of disease, skin Or dark brown discoloration, severe skin cracks can occur. 5.2 pairs antibody sandwich enzyme-linked immune assay The use of double-antibody sandwich enzyme-linked immunosorbent assay samples were screened (see Appendix B), if the test result is positive, the need for pathogens ......

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