SN/T 0331-2013 English PDF

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SN/T 0331-2013: Method for the inspection of bacillus anthrax in animal by-products for export
Status: Valid

SN/T 0331: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 0331-2013	419	Add to Cart	3 days	Method for the inspection of bacillus anthrax in animal by-products for export	Valid
SN 0331-1994	439	Add to Cart	4 days	Methods for the inspection of bacillus anthraxin animal by-products for export	Obsolete

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GB/T 10288   NY/T 1663   GB/T 7740   SN/T 5290   SN/T 0940   SN/T 0844   

Basic data

Standard ID: SN/T 0331-2013 (SN/T0331-2013)
Description (Translated English): Method for the inspection of bacillus anthrax in animal by-products for export
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B45
Classification of International Standard: 65.020.30
Word Count Estimation: 16,123
Older Standard (superseded by this standard): SN 0331-1994
Quoted Standard: GB 19489
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the export tinplate cans boiled bamboo shoots sampling, inspection and test results judgment. This standard applies to a capacity greater than or equal to the export inspection boiled bamboo shoots 9L of tin cans.

SN/T 0331-2013: Method for the inspection of bacillus anthrax in animal by-products for export

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for the inspection of bacillus anthrax in animal by-products for export People's Republic of China Entry-Exit Inspection and Quarantine Standards Replacing SN 0331-1994 Exports of livestock products in Bacillus anthracis test methods Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces SN 0331-1994 "export of livestock products in Bacillus anthracis test methods." This standard compared with SN 0331-1994, major technical changes are as follows. --- Remove the soybean lectin absorption experiments, phage lysis test, beads and animal experiments; --- Increased capsular staining, Ascoli reaction and polymerase chain reaction (PCR) test. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Inner Mongolia, People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Wang Haiyan, Ao Weihua, Zhao Zhiguo, Zhaolin Li, Guo Tie Cheng, Feng Yongsheng. This standard replaces the standards previously issued as follows. --- SN 0331-1994. Exports of livestock products in Bacillus anthracis test methods

1 Scope

This standard specifies the export of livestock anthrax testing. This standard is applicable to the export mane, wool, cashmere, wild animal fur, raw bone particles and other animal products in Bacillus anthracis test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety

3 biosafety

Anthrax is a zoonotic disease, the test environment and execution protection requirements in accordance with GB 19489.

4 media and reagents

4.1 sterile defibrinated sheep blood (or horse blood). 4.2 anthrax precipitin standard antigen. 4.3 anthrax precipitin positive sera. 4.4 anthrax precipitin negative serum. 4.5 0.7% sodium bicarbonate nutrient agar plate. A A.1 in the appendix. 4.6 ordinary nutrient broth. see A.2. 4.7 ordinary nutrient agar plate. see A.3. 4.8 pentamidine and polymyxin agar plates. see A.4. 4.9 PLET agar plates. see A.5. 4.10 basic methylene blue staining solution. see A.6. 4.11 Gram stain solution. see A.7. 4.12 Wash sample solution. see A.8. 4.13 0.5% phenol saline. see A.9. 4.14 Electrophoresis grade agarose powder. 4.15 TaqDNA polymerase. 5U/μL. 4.16 100bpDNAladderMarker (DNA molecular weight standard). 4.17 10-fold concentration TaqDNA polymerase buffer (10 × TapBuffer). 15mmol/LMgC1. 4.18 dNTP mixture. deoxyadenosine triphosphate (dATP), deoxythymidine triphosphate (dTTP), deoxyguanosine triphosphate (dGTP), off Oxygen triphosphate (dCTP) each 2.5mmol/L. 4.19 50 × TAE electrophoresis buffer. see A.10. 4.20 6 × loading buffer. see A.11. ......

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