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NY/T 724-2022 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. NY/T 724-2022: (Determination of Lasalocid Sodium in Feed - High Performance Liquid Chromatography) Status: Valid NY/T 724: Evolution and historical versions
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NY/T 724-2003: Determination of lasalocid in feeds. High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of lasalocid in feeds.High performance liquid chromatography ICS 65.120 B20 People's Republic of China Agricultural Industry Standard Determination of rasakalil sodium in feed High performance liquid chromatography Released on December 12,.2003 2004-03-01 implementation Published by the Ministry of Agriculture ForewordThis standard is based on the general analysis methods recommended by the National Feed Association (NFIA) and a large number of domestic and foreign literature. According to the research of China's technological development level, high performance liquid chromatography (HPLC)-fluorescence detection method was adopted. This standard was proposed by the Ministry of Agriculture of the People's Republic of China and the National Feed Industry Standardization Technical Committee. This standard is under the jurisdiction of the National Feed Industry Standardization Technical Committee. This standard was drafted. National Veterinary Drug Evaluation Center (National Agricultural University Animal Medical College). The main drafters of this standard. Shen Jianzhong, Zhang Suxia, Liu Jinfeng, and Yu Yuming. Determination of rasakalil sodium in feed High performance liquid chromatography1 ScopeThis standard specifies a high performance liquid chromatography method for the determination of the content of rasakoxicil sodium in animal feed. This standard applies to the determination of the content of rasakhicil sodium in compound feed, concentrated feed and additive premix feed. Detection limit of the method It is 5 mg/kg.2 Normative referencesThe terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (excluding errata content) or modified versions are not suitable for this standard. However, it is encouraged whether the parties that have reached an agreement under this standard may Use the latest version of these files. For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 14699.1 Feed sampling method3 Principle of the methodExtraction of rasakalil sodium from the sample with methanol, using phosphate buffer-acetonitrile-methanol as the mobile phase, using high performance liquid chromatography---fluorescence Photodetection separation assay.4 reagents and solutionsUnless otherwise stated, the reagents used in this method are of analytical grade and the water is deionized water, which meets the requirements of GB/T 6682 secondary water. 4.1 Acetonitrile. chromatographically pure. 4.2 Methanol. chromatographically pure. 4.3 Phosphate buffer (pH=7). Accurately weigh 3.121g of sodium dihydrogen phosphate (NaH2PO4·2H2O) and 7.164g of hydrogen phosphate Sodium (Na2HPO4·12H2O), dissolved in water, and made up to 1000 mL, to obtain a concentration of 犮(NaH2PO4·2H2O)=0.02 mol/L phosphorus Sodium dihydrogen sodium solution (solution A) and concentration 犮 (Na2HPO4·12H2O) = 0.02 mol/L. Disodium hydrogen phosphate solution (solution B). Put liquid A with Mix B solution in a ratio of 3.9 + 6.1 to prepare a phosphate buffer (pH = 7). 4.4 Lazalocir sodium standard solution. 4.4.1 Lazalocir sodium standard stock solution. Accurately weigh the standard dose of rasakalil sodium (purity ≥98%) 0.1000g, placed in 100mL capacity In the measuring flask, the solution was dissolved in methanol, and the volume was 1000 μg/mL, and stored in a refrigerator at 4 ° C. 4.4.2 Lazalocix sodium standard working solution. accurately absorb a certain amount of standard stock solution (4.4.1), and put it in a 10mL volumetric flask. Methanol was diluted and made up to a concentration of 2.5 μg/mL, 5.0 μg/mL, 7.5 μg/mL, 10.0 μg/mL, and 12.5 μg/mL. Standard working solution of 17.5 μg/mL.5 Instruments and equipmentInstruments and equipment commonly used in laboratories. 5.1 High Performance Liquid Chromatograph. equipped with a fluorescence detector. 5.2 Centrifuge. 5.3 Oscillator. ...... |