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GHT1386-2022 PDF English

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GHT1386-2022: Determination of lutein, zeaxanthin, cryptoxanthin and carotene in food from fruits and vegetables
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GH/T 1386-2022English249 Add to Cart 3 days [Need to translate] Determination of lutein, zeaxanthin, cryptoxanthin and carotene in food from fruits and vegetables

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Basic data

Standard ID GH/T 1386-2022 (GH/T1386-2022)
Description (Translated English) Determination of lutein, zeaxanthin, cryptoxanthin and carotene in food from fruits and vegetables
Sector / Industry Supply and Marketing Cooperatives Industry Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 10,111
Date of Issue 2022-11-24
Date of Implementation 2023-01-01
Issuing agency(ies) All-China Federation of Supply and Marketing Cooperatives

GHT1386-2022: Determination of lutein, zeaxanthin, cryptoxanthin and carotene in food from fruits and vegetables




---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67:050 CCSX04 Industry Standards for Supply and Marketing Cooperation of the People's Republic of China Lutein, zeaxanthin, cryptoxanthin and Determination of carotene fruits and vegetables 2023-01-01 Implementation Released by All China Federation of Supply and Marketing Cooperatives Published by China Standard Press

foreword

This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting: Please note that some contents of this document may refer to patents: The issuing agency of this document assumes no responsibility for identifying patents: This document was proposed by the All-China Federation of Supply and Marketing Cooperatives: This document is under the jurisdiction of the National Fruit Standardization Technical Committee Storage and Processing Sub-Technical Committee (SAC/TC501/SC1): This document was drafted by: Jiangsu Academy of Agricultural Sciences, All-China Federation of Supply and Marketing Cooperatives Nanjing Institute of Comprehensive Utilization of Wild Plants, Xinghua City Lianfu Food Co:, Ltd:, Jiangsu Alande Nutritional Products Co:, Ltd:, Pony Testing Group Jiangsu Co:, Ltd:, National Vegetable Processing Technology Research Institute Development Professional Branch Center, Jiangsu Province Dehydrated Fruit and Vegetable Industry Technology Innovation Strategic Alliance, Anhui Science and Technology Research Institute, Dandong Agricultural and Rural Development Service Center, Jiangsu Shipu Testing Service Co:, Ltd: The main drafters of this document: Li Dajing, Wang Songjun, Dai Zhuqing, Zhang Fenglun, Shi Zuhao, Zhang Zhongyuan, Zhang Xing, Liu Qingzheng, He Weiwei, Xiao Yadong, Tu Qi, Zhang Guodong, Nie Meimei, Yao Zhengying, Zhang Xiaohong, Liu Qingrong, Xu Shuwen, Liu Yujun, Wang Xiaodong, Ma Haixia: Lutein, zeaxanthin, cryptoxanthin and Determination of carotene

1 Scope

This document describes the high efficacy of lutein, zeaxanthin, α-cryptoxanthin, β-cryptoxanthin, α-carotene and β-carotene in fruit and vegetable foods: Liquid Chromatographic Determination Method: This document is applicable to fruit and vegetable raw materials and processed products containing carotenoids:

2 Normative references

This document has no normative references:

3 Terms and Definitions

This document does not have terms and definitions that need to be defined:

4 principles

The test sample was extracted by n-hexane-ethanol-acetone-toluene compound solution, and saponified by potassium hydroxide-methanol solution to make lutein, zeaxanthin, α- Cryptoxanthin, β-cryptoxanthin, α-carotene, and β-carotene were released in free form, extracted with n-hexane and concentrated under reduced pressure, then subjected to HPLC Determination by chromatography and quantification by external standard method:

5 Reagent materials and equipment

5:1 Reagents 5:1:1 Methyl tert-butyl ether [CH3OC(CH3)3, MTBE]: chromatographically pure: 5:1:2 Methanol (CH4O): chromatographically pure: 5:1:3 Dichloromethane (CH2Cl2): chromatographically pure: 5:1:4 n-Hexane (C6H14): chromatographically pure: 5:1:5 Double distilled water (H2O): first-grade water: 5:1:6 Absolute ethanol (C2H6O): analytically pure: 5:1:7 Acetone (C3H6O): analytically pure: 5:1:8 Toluene (C7H8): analytically pure: 5:1:9 Anhydrous sodium sulfate (Na2SO4): analytically pure: 5:1:10 Potassium hydroxide (KOH): analytically pure: 5:1:11 Ascorbic acid (C6H8O6): analytically pure: 5:1:12 2,6-di-tert-butyl-4-methylphenol (C15H24O, BHT): analytically pure:
...

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