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Basic data
| Standard ID | GH/T 1315-2020 (GH/T1315-2020) |
| Description (Translated English) | (Determination of casein in honey enzyme-linked immunoassay) |
| Sector / Industry | Supply and Marketing Cooperatives Industry Standard (Recommended) |
| Classification of Chinese Standard | X31 |
| Word Count Estimation | 5,520 |
| Date of Issue | 2020-12-07 |
| Date of Implementation | 2021-03-01 |
| Regulation (derived from) | China National Supply and Marketing Cooperatives Announcement No. 2 of 2020 |
| Issuing agency(ies) | All-China Federation of Supply and Marketing Cooperatives |
GHT1315-2020: (Determination of casein in honey enzyme-linked immunoassay)
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of casein in honey enzyme-linked immunoassay)
ICS 67.180.10
CCSX31
Industry Standards for Supply and Marketing Cooperation of the People's Republic of China
Enzyme-linked immunosorbent assay for the determination of casein in honey
2020-12-07 released
2021-03-01 implementation
Released by All China Supply and Marketing Cooperatives
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document was proposed by the All-China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Bee Product Standardization Working Group (SAC/SWG2).
This document was drafted by. Qinhuangdao Customs Technology Center, Shanghai Guanshengyuan Bee Products Co., Ltd.
The main drafters of this document. Wang Haiyang, Liu Jiqin, Gao Fei, Qian Yunkai, Cui Zongyan, Zhang Ang, Zhang Jinjie, Wu Xi, Wang Lishan, Li Liang, Sun Ping.
Enzyme-linked immunosorbent assay for the determination of casein in honey
1 Scope
This document specifies the enzyme-linked immunoassay method for casein in honey.
This document is applicable to the determination of casein in acacia honey, rape honey, linden tree honey, and vitex honey.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
The casein in the sample binds to the specific antibody coated on the microplate to form an antibody-antigen complex. Add enzyme-labeled antibody after washing the plate,
The formation of antibody-antigen-enzyme-labeled antibody complexes ("sandwich" method). Then add the substrate to develop color, and measure the absorption at 450nm with a microplate reader.
Photometric value, according to the absorbance value to get the content of casein in the sample, the absorbance value is proportional to the content of casein in the sample.
5 Reagents and materials
5.1 Water
According to the first grade water specified in GB/T 6682.
5.2 Casein-linked immunoassay kit
Commercial kit (RIDASCREEN.FASTCasein)1). includes 48-well microplate, casein standard 0μg/mL,
0.5μg/mL, 1.5μg/mL, 4.5μg/mL, 13.5μg/mL, substrate/color reagent, stop solution, enzyme-labeled antibody dilution buffer, 11×enzyme-labeled antibody
Body (dilute with enzyme-labeled antibody dilution buffer at a volume ratio of 1.11 before use), 10× sample extraction buffer (dilute with 10 times distilled water before use)
Release), 10× wash buffer (dilute with 10 times distilled water before use).
6 Apparatus and equipment
6.1 Water bath. temperature 60℃±1℃.
1) The information is given for the convenience of the users of this document, and does not mean the approval of a certain product operation procedure. If the operation steps of other products are different
The same, it must be used after experimental evaluation.
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