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Diagnostic techniques for haemonchosis
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Basic data
| Standard ID | GB/T 47205-2026 (GB/T47205-2026) |
| Description (Translated English) | Diagnostic techniques for haemonchosis |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 26,226 |
| Date of Issue | 2026-02-27 |
| Date of Implementation | 2026-09-01 |
| Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China |
GB/T 47205-2026: Diagnostic techniques for haemonchosis
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB 41
National Standards of the People's Republic of China
Diagnostic techniques for schistosomiasis
Published on 2026-02-27
Implemented on 2026-09-01
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
Introduction IV
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Abbreviations 1
5.Clinical Diagnosis 1
6.Sample Collection and Processing 2
7.Etiological diagnosis 3
8.Indirect ELISA detection 4
9 PCR detection 5
10.Comprehensive Judgment 7
Appendix A (Informative) Typical Clinical Symptoms of Schistosomiasis 8
Appendix B (Standardized) Preparation Methods for Pathogen Diagnostic Reagents 9
Appendix C (Informative) Morphological Characteristics of Haemonchus nematodes 10
Appendix D (Informative) Micrometry Techniques 12
Appendix E (Informative) Preparation and Characterization of Recombinant HcADRM Protein 14
Appendix F (Normative) Preparation of Reagents and Materials for Indirect ELISA and PCR Methods 16
Appendix G (Informative) PCR detection primer sequences and electrophoresis diagrams 18
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by Nanjing Agricultural University and Jiangsu Vocational College of Agriculture and Forestry.
The main drafters of this document are. Yan Ruofeng, Li Xiangrui, Xu Lixin, Song Xiaokai, Lu Mingmin, and Bu Yongqian.
Introduction
Haemonchus nematode disease is a common gastrointestinal parasitic disease in ruminants such as cattle and sheep. In my country, it is classified as a Class III animal disease. The pathogen of this disease...
Haemonchus longistipes is a common nematode in my country.
The adult Haemonchus nematode parasitizes the fourth stomach of ruminants, feeding on the host's blood. The adult worms lay eggs, which are excreted in the feces. The eggs then hatch in suitable environments.
Under suitable conditions, the first-stage larvae hatch, and after two molts, they develop into infectious third-stage larvae, which are fed on ruminants.
Infection occurs through the entry of the third-stage larvae, which then further develop into fourth-stage, fifth-stage, and adult larvae in the fourth stomach. Young animals such as lambs are susceptible.
Infection can lead to anemia, diarrhea, weight loss, and even death; in adult animals, infection can result in slow growth and reduced feed efficiency.
Diagnostic techniques for schistosomiasis
1 Scope
This document describes the clinical diagnosis, etiological diagnosis, indirect ELISA, PCR, and other diagnostic methods for schistosomiasis.
This document applies to the diagnosis, quarantine, monitoring, and epidemiological investigation of haemospirochetosis in ruminants.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation
3 Terms and Definitions
This document does not contain any terms or definitions that need to be defined.
4.Abbreviations
The following abbreviations apply to this document.
bp. Base pair
DMSO. Dimethyl sulfoxide
TAE. Tris-Acetate-EDTA (Tris-hydroxymethylaminomethane-acetic acid-ethylenediaminetetraacetic acid)
TBST. Tris-buffered saline-Tween-20 solution
5.Clinical Diagnosis
5.1 Popular Characteristics
5.1.1 Susceptible animals mainly include ruminants such as cattle, sheep, camels, deer, and alpacas.
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