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Inspection of grain and oils - Determination of water-soluble protein in soya bean
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Basic data
| Standard ID | GB/T 46117-2025 (GB/T46117-2025) |
| Description (Translated English) | Inspection of grain and oils - Determination of water-soluble protein in soya bean |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B20 |
| Classification of International Standard | 67.050 |
| Word Count Estimation | 10,123 |
| Date of Issue | 2025-08-29 |
| Date of Implementation | 2026-03-01 |
| Issuing agency(ies) | State Administration for Market Regulation, National Standardization Administration |
GB/T 46117-2025: Inspection of grain and oils - Determination of water-soluble protein in soya bean
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT46117-2025
ICS 67.050
CCSB20
National Standard of the People's Republic of China
Grain and oil inspection-Determination of water-soluble protein content in soybeans
Released on August 29, 2025
Implementation on March 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was submitted by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Standardization of Cereals and Oils (SAC/TC270).
This document was drafted by. Nanjing University of Finance and Economics, China National Grain and Oils Corporation Zhenjiang Quality Inspection Center Co., Ltd., Heilongjiang Provincial Grain Quality and Safety Monitoring and Technology
Technical Center, China National Grain and Oils Corporation Chengdu Storage Research Institute Co., Ltd., Jiangsu Grain and Oils Quality Monitoring Center, COFCO Donghai Grain and Oils Industry (Zhangjiagang) Co., Ltd.
Company, Fangchenggang Aojia Grain and Oil Industry Co., Ltd.
The main drafters of this document are Yuan Jian, Zhou Zhou, Ji Lanyang, Zhang Liuping, Xing Changrui, Yang Chao, Jia Jirong, Dong Hua, Qin Jingwen, Li Yuanxin, and Zhang Hui.
Grain and oil inspection-Determination of water-soluble protein content in soybeans
1 Scope
This document describes the principle of determining the content of water-soluble protein in soybeans, and specifies the reagents and materials, instruments and equipment, analytical procedures, results,
Calculation and precision.
This document is applicable to the determination of water-soluble protein content in soybeans.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 601 Preparation of standard titration solutions for chemical reagents
GB/T 5491 Sampling and separation methods for inspection of grain and oilseeds
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Principles
Water-soluble protein in soybeans is extracted with water, and nitrogenous compounds are decomposed under catalytic heating conditions, and the generated ammonia combines with sulfuric acid to form
Ammonium sulfate, add alkali to distill to release ammonia, absorb it with boric acid and titrate it with sulfuric acid or hydrochloric acid standard solution, calculate the nitrogen content based on the acid consumption
The amount of water-soluble protein in soybeans can be calculated by multiplying it by the conversion coefficient.
5 Reagents and Materials
Unless otherwise stated, all reagents used in this document were of analytical grade.
5.1 Reagents
5.1.1 Copper sulfate pentahydrate (CuSO4·5H2O).
5.1.2 Potassium sulfate (K2SO4).
5.1.3 Sulfuric acid (H2SO4).
5.1.4 Hydrochloric acid (HCl).
5.1.5 Boric acid (H3BO3).
5.1.6 Methyl red (C15H15N3O2).
5.1.7 Bromocresol green (C21H14Br4O5S).
5.1.8 Sodium hydroxide (NaOH).
...
