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Basic data
| Standard ID | GB/T 44480-2024 (GB/T44480-2024) |
| Description (Translated English) | Chemicals - Eremias argus acute oral toxicity test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | A80 |
| Classification of International Standard | 13.300 |
| Word Count Estimation | 14,167 |
| Date of Issue | 2024-09-29 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44480-2024: Chemicals - Eremias argus acute oral toxicity test
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Chemicals - Eremias argus acute oral toxicity test
Acute oral toxicity test of chemicals on Lizard
ICS 13.300
CCS A 80
National Standard of the People's Republic of China
Released on 2024-09-29
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Experimental Overview ... 1
5 Test methods ... 2
5.1 Materials and Instruments 2
5.2 Sample preparation 2
5.3 Test operation 2
5.4 Result calculation and presentation 3
5.5 Quality Assurance and Quality Control ... 3
6 Test Report ... 3
Appendix A (Informative) Conditions and methods for domestication and reproduction of the beautiful spotted lizard A Method ... 4
Appendix B (Informative) Solution Preparation and Test Observation Record Sheet ... 7
References ... 8
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee on Hazardous Chemicals Management Standardization (SAC/TC 251).
This document was drafted by. Eco-Environmental Research Center of the Chinese Academy of Sciences, China Inspection and Testing (Tianjin) Co., Ltd., China
Institute of Inspection and Quarantine Science.
The main drafters of this document are. Chang Jing, Wang Huili, Wan Bin, Xie Yun, Li Jianzhong, Zhou Lili, Liu Chunyan, and Wang Lili.
Acute oral toxicity test of chemicals on Lizard
1 Scope
This document describes the test method for the acute oral toxicity test of chemicals to Lizard lizards, and specifies the test materials, conditions, operations,
Basic requirements for quality control, data processing, test reports, etc.
This document is applicable to testing the acute toxic effects of chemicals on the beautiful spotted lizard.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 31270.9-2014 Test Guidelines for Environmental Safety Assessment of Chemical Pesticides Part 9.Acute Toxicity Test for Birds
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
median lethal dose; LD50
The statistical dose of a toxicant that causes half of the animals to die after a single administration of the test substance to experimental animals.
The mass of the test substance received per unit body weight is expressed.
3.2
Maximum non lethal dose; LD0
The highest toxic dose that does not cause death to experimental animals after a single administration of the test substance to experimental animals.
Body weight is expressed as the mass of the test substance.
3.3
Minimum total lethal dose; LD100
The lowest statistical dose of a toxicant that causes all death of experimental animals after a single administration of the test substance to experimental animals.
The body weight is expressed as the mass of the test substance received.
3.4
acute oral toxicity
Health-damaging effects that occur in experimental animals within a short period of time (7 days) after a single oral administration of a test substance.
3.5
Test substances
A pure substance or mixture composed of various elements.
4 Experimental Overview
A series of test substances with different concentrations were orally infused into the beautiful spotted lizard once. The test period was 7 days, and the test data were recorded every day.
...
