GB/T 39729-2020 English PDFUS$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 39729-2020: General requirements for measurement of cell purity - Flow cytometry Status: Valid
Basic dataStandard ID: GB/T 39729-2020 (GB/T39729-2020)Description (Translated English): General requirements for measurement of cell purity - Flow cytometry Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C27 Classification of International Standard: 07.080 Word Count Estimation: 10,152 Date of Issue: 2020-12-14 Date of Implementation: 2021-07-01 Regulation (derived from): National Standard Announcement No. 28 of 2020 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 39729-2020: General requirements for measurement of cell purity - Flow cytometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(General requirements for cell purity determination Flow cytometry) ICS 07:080 CCSC27 National Standards of People's Republic of China General requirements for cell purity determination Flow cytometry 2020-12-14 release 2021-07-01 implementation State Administration for Market Regulation Issued by the National Standardization Management Committee Table of contentsForeword Ⅰ Introduction Ⅱ 1 Scope 1 2 Normative references 1 3 Terms, definitions and abbreviations 1 4 Method principle 1 5 General requirements 2 5:1 Reagents or materials 2 5:2 Equipment 2 5:3 Sample preparation 3 5:4 Test Step 3 5:5 Data Analysis 5 5:6 Method confirmation 5 5:7 Report 5 Reference 6ForewordThis document is in accordance with the provisions of GB/T 1:1-2020 Standardization Guidelines Part 1: Structure and Drafting Rules of Standardization Documents Drafting: Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying patents: This document was proposed and managed by the National Standard Material Research Center: Drafting organization of this document: Chinese Academy of Metrology: The main drafters of this document: Liu Yingying, Wang Jing, Chen Chuan, Fu Boqiang, Yuan Baozhu, Niu Chunyan, Sui Zhiwei:IntroductionCell purity is the basic parameter for evaluating and analyzing the characteristics of cell samples and the quality of cell products: Therefore, medical testing, biomedical research and development, Fields such as production and quality inspection require extensive measurement of cell purity: Flow cytometry can be performed on single particles or cells suspended in liquid High-speed, multi-parameter analysis is a commonly used method for cell purity measurement: In order to realize measurement systems for different laboratories, platforms, operators, etc: The consistency of data between conditions requires standardization of the technical aspects of cell purity testing by flow cytometry: General requirements for cell purity determination Flow cytometry1 ScopeThis document describes the terms, definitions, abbreviations, method principles, and reagents or materials, instrument General requirements for equipment, sample preparation, test procedures, data analysis, method validation and reporting: This document is applicable to the determination of cell purity by flow cytometry in research, production and testing:2 Normative referencesThere are no normative references in this document: 3 Terms, definitions and abbreviations 3:1 Terms and definitions The following terms and definitions apply to this document: 3:1:1 Target cell celoftestingtarget Consistent with the detection target, cells with characteristics (such as size, number, and specific markers) that can be detected by flow cytometry: 3:1:2 Cell purity celpurity The percentage of target cells in the total number of cells in the sample: 3:1:3 Cell staining A technique in which specific components of cells are combined with fluorescent dyes or specific antibodies labeled with fluorescent dyes for detection: Note: The specific components are protein, DNA, RNA, etc: 3:2 Abbreviations The following abbreviations apply to this document: FSC: Forward Scatter (ForwardScatter) SSC: Side Scatter (SideScatter)4 Principle of the methodUsing a flow cytometer, the total cells in the sample to be tested are determined by the different optical (scattered light and fluorescence) characteristics of the total cells and the target cells Distinguish and count the target cells: According to the measured number of total cells and target cells, the purity of the target cells is calculated: ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 39729-2020_English be delivered?Answer: Upon your order, we will start to translate GB/T 39729-2020_English as soon as possible, and keep you informed of the progress. 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