GB/T 39730-2020 English PDFUS$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 39730-2020: General requirements for cell counting - Flow cytometry Status: Valid
Basic dataStandard ID: GB/T 39730-2020 (GB/T39730-2020)Description (Translated English): General requirements for cell counting - Flow cytometry Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C27 Classification of International Standard: 07.080 Word Count Estimation: 10,139 Date of Issue: 2020-12-14 Date of Implementation: 2021-07-01 Regulation (derived from): National Standard Announcement No. 28 of 2020 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 39730-2020: General requirements for cell counting - Flow cytometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(General requirements for cell counting Flow cytometry) ICS 07:080 CCSC27 National Standards of People's Republic of China General requirements for cell counting Flow cytometry 2020-12-14 release 2021-07-01 implementation State Administration for Market Regulation Issued by the National Standardization Management Committee Table of contentsForeword Ⅰ Introduction Ⅱ 1 Scope 1 2 Normative references 1 3 Terms, definitions and abbreviations 1 4 Method principle 2 5 General requirements 2 5:1 Reagents or materials 2 5:2 Equipment 3 5:3 Sample preparation 3 5:4 Test Step 4 5:5 Data Analysis 6 5:6 Method confirmation 6 5:7 Report 6 Reference 7ForewordThis document is in accordance with the provisions of GB/T 1:1-2020 Standardization Guidelines Part 1: Structure and Drafting Rules of Standardization Documents Drafting: Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying patents: This document was proposed and managed by the National Standard Material Research Center: Drafting organization of this document: Chinese Academy of Metrology: The main drafters of this document: Liu Yingying, Wang Jing, Fu Boqiang, Yuan Baozhu, Niu Chunyan, Sui Zhiwei, Chen Chuan:IntroductionFlow cytometry is a technique for high-speed, multi-parameter analysis of single particles or cells suspended in liquid: Can be opposed by flow cytometry Direct counting of the cells in the sample preparation can not only achieve the total cell count in the sample, but also perform typing and counting according to cell characteristics: number: In order to achieve data consistency between measurement system conditions such as different laboratories, platforms, operators, etc:, it is necessary to perform flow cytometry Standardize the technical aspects of the cell counting test: General requirements for cell counting Flow cytometry1 ScopeThis document describes the terms, definitions, abbreviations, method principles, and reagents or materials, instrument General requirements for equipment, sample preparation, test procedures, data analysis, method validation and reporting: This document is applicable to cell counting performed by flow cytometry in research, production and testing:2 Normative referencesThere are no normative references in this document: 3 Terms, definitions and abbreviations 3:1 Terms and definitions The following terms and definitions apply to this document: 3:1:1 Celcounting Measurement of cell number: [Source: ISO 20391-1:2018,3:8] 3:1:2 Cell staining A technique in which specific components of cells are combined with fluorescent dyes or specific antibodies labeled with fluorescent dyes for detection: Note: The specific components are protein, DNA, RNA, etc: 3:1:3 Certified reference material; CRM Attached with documents issued by an authoritative organization, providing one or more characteristics with uncertainty and traceability obtained using effective procedures The value of the standard substance: [Source: JJF1001-2018, 8:15] 3:1:4 Accuracy Measurementaccuracy The degree of agreement between the measured value and its true value: [Source: JJF1001-2018, 5:8, with modification] 3:1:5 Precision Measurementprecision Under specified conditions, the degree of agreement between the displayed value or the measured value of the same or similar measured object: [Source: JJF1001-2018, 5:10, with modification] ...... |
