GB/T 36845-2018 English PDF

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GB/T 36845-2018: Detection and identification of Spiroplasma citri Saglio et al
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Basic data

Standard ID: GB/T 36845-2018 (GB/T36845-2018)
Description (Translated English): Detection and identification of Spiroplasma citri Saglio et al
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,188
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36845-2018: Detection and identification of Spiroplasma citri Saglio et al

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Spiroplasma citri Saglio et al ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of citrus stubborn disease snail Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard is drafted by. Nanhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Guangdong Entry-Exit Inspection of the People's Republic of China Epidemic Bureau, Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Yantai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, and the People's Republic of China Hunan Hunan Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Tan Yi, Li Kaibing, Liu Haijun, Zhang Jingxuan, Li Jinqing, Zhou Huiping, Liu Xiaohong, Li He. Method for quarantine and identification of citrus stubborn disease snail

1 Scope

This standard specifies the quarantine and identification methods for the citrus stubborn sporozoite Spiroplasmacitri Saglioetal in the entry phytosanitary. This standard applies to grapefruit, lemon, orange, orange and acid orange and other citrus fruits, seedlings and insect mediators. Quarantine and identification of snails in stubborn disease of orange.

2 Basic information

Chinese name. citrus stubborn disease spiroplasma Scientific name. SpiroplasmacitriSaglioetal English name. Critusstubborndisease, Littleleafdiseaseofcitrus, Stubborndiseaseofcitrus English abbreviation. CSD Classification status. genus Mycoplasma pathogens, Bacteria, Tenericumes, Molicutes, insects Entomoplasmatales, Spiroplasmataceae, Spiroplasma. See Appendix A for additional information on citrus snails.

3 Principle of the method

According to the biological characteristics of snail snails, the specific primers PCR amplification and sequence determination results, real-time PCR detection As a result, the pathogen species was determined.

4 instruments and main reagents

4.1 Main equipment General PCR instrument, fluorescence PCR instrument, ultra-clean workbench, sterilization pot, ice machine, nucleic acid protein analyzer, high-speed refrigerated centrifuge, table Small centrifuges, ultra-low temperature refrigerators, conventional refrigerators, vortex shakers, micro-samplers, electrophoresis systems, gel imaging systems, microscopes. 4.2 Primary reagents and media All reagents were of analytical grade unless otherwise stated. PCR buffer, dNTPs (dATP, dTTP, dCTP, dGTP), GoTaq DNA polymerase, primers and probes. See Appendix D for the preparation method of LD8 medium used in the test.

5 detection and identification methods

5.1 Symptom observation Carefully observe citrus seedlings, fruits, leaves, etc. See Appendix A for symptom descriptions. ......

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