GB/T 28718-2012 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28718-2012: Determination of T-2 toxin in feed -- High performance liquid chromatography with immunoaffinity column clean-up Status: Valid
Basic dataStandard ID: GB/T 28718-2012 (GB/T28718-2012)Description (Translated English): Determination of T-2 toxin in feed -- High performance liquid chromatography with immunoaffinity column clean-up Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 7,759 Quoted Standard: GB/T 6682; GB/T 14699.1 Regulation (derived from): National Standards Bulletin No. 24 of 2012 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the feed content of T-2 toxin immunoaffinity column clean high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed in the T-2 toxin content. This standard method dete GB/T 28718-2012: Determination of T-2 toxin in feed -- High performance liquid chromatography with immunoaffinity column clean-up---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. ICS65.120 B46 National Standards of People's Republic of China Determination feed T-2 toxin Immunoaffinity column clean - High Performance Liquid Chromatography Issued on. 2013-02-01 2012-09-03 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard rule according to GB/T 1.1-2009 given draft. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points. This standard was drafted. Shanghai Feed Quality Supervision, Inspection Station, the Shanghai Academy of Agricultural Sciences Agricultural Products Quality Standards and Testing Technology The Institute of Feed Science, Zhejiang University. The main drafters of this standard. Zhao Zhihui, Lin Miao, T. (MONTHLY) Lu Tianhua, Gu race red, Mao Feng Xi, Wu Aibo. Determination feed T-2 toxin Immunoaffinity column clean - High Performance Liquid Chromatography1 ScopeThis standard specifies the feed content of T-2 toxin immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, feed Determination of T-2 toxin content in concentrate. The standard method detection limit of 10μg/kg, the limit of quantification of 30μg/kg.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated edition applies to this article Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples Principle 3 With a mixed solution of methanol and water to extract a sample of T-2 toxin, by immunoaffinity column cleanup after 1- nitrile anthracene derivatized by high performance liquid chromatography Spectral fluorescence detector, external standard.4 Reagents and materialsUnless otherwise specified in the analysis using only water as a confirmed analytical reagents and in line with GB/T 6682 stipulated. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. 4.3 Toluene. chromatography. 4.4 methanol-water (8020). Take 80mL methanol was added 20mL of water. 4.5 4-dimethylaminopyridine (DMAP, 0.325g/L). Weigh accurately 0.0325g4- dimethylaminopyridine, dissolved with toluene and is scheduled Volume to 100mL. 4.6 1- anthracene nitrile (1-anthroylnitrile, 1-AN, 0.3g/L). Weigh 0.030g1- anthracene nitrile, toluene was dissolved and set the volume to 100mL. 4.7 T-2 toxin standards. purity greater than or equal to 98%. 4.8 T-2 toxin stock standard solution. Accurately weigh an appropriate amount of T-2 toxin standard (accurate to 0.0002g), dubbed the concentration of acetonitrile 0.5mg/mL of standard stock solution, -20 ℃ refrigerator in the dark, valid for three months. Before using acetonitrile diluted to the appropriate concentration Standard working solution. 4.9 T-2 toxin standard working solution. Depending on needs, accurately draw a certain amount of T-2 toxin standard stock solution was diluted with acetonitrile, respectively Dubbed the equivalent of 10ng/mL, 50ng/mL, 100ng/mL, 150ng/mL, working standard solution 200ng/mL of, 4 ℃ preservation, valid Of 7d. 4.10 T-2 toxin immunoaffinity column. column capacity greater than or equal 2μg. 4.11 glass fiber filter. diameter 11cm, pore size 1.5μm, no fluorescent properties. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28718-2012_English be delivered?Answer: Upon your order, we will start to translate GB/T 28718-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 28718-2012_English with my colleagues?Answer: Yes. 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