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GB/T 28715-2012 English PDF

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US$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 28715-2012: Determination of acidic and neutral protease activity in feed additives -- Spectro-photometric method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28715-2012	189	Add to Cart	3 days	Determination of acidic and neutral protease activity in feed additives -- Spectro-photometric method	Valid

Similar standards

GB/T 31216 GB/T 31217 GB/T 28717 GB/T 28718 GB/T 28716

Basic data

Standard ID: GB/T 28715-2012 (GB/T28715-2012)
Description (Translated English): Determination of acidic and neutral protease activity in feed additives -- Spectro-photometric method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 8,824
Quoted Standard: GB/T 6682
Regulation (derived from): National Standards Bulletin No. 24 of 2012
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the feed additive acidic and neutral protease activity measurement method. This standard applies to feed additives acidic, neutral protease products Determination of enzyme activity and enzyme products Determination of neutral prot

GB/T 28715-2012: Determination of acidic and neutral protease activity in feed additives -- Spectro-photometric method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of acidic and neutral protease activity in feed additives. Spetrophotometric method ICS 65.120 B46 National Standards of People's Republic of China Determination of feed additives acidic, neutral proteinase activity Issued on. 2012-09-03 2013-02-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard was drafted. Ministry of Agriculture and genetically modified products quality and safety supervision and Testing Center (Hangzhou), Zhejiang University Feed Division Academy of Sciences, Wuhan Xinhua Yang biological Limited. The main drafters of this standard. Wang Li, Liu Aichun, Zou Xiaoting, Wu Qi, Zhao Yun, Zhu Jia Hong, Chen Xiaoli, Zhou Ying, Xie Hongyun. Determination of feed additives acidic, neutral proteinase activity

1 Scope

This standard specifies the method for determination of feed additives acidic and neutral proteinase activity. This standard applies to feed additives acidic, neutral protease product was measured in the enzyme activity, and the enzyme product neutral protease activity Determination of force. The standard limit of quantification was 500U/g (U/mL).

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Acidic, neutral protease activity unit acidicandneutralproteaseactiveunit In (pH 3.0 acid protease, neutral protease pH 7.2) constant temperature (40 ℃ ± 0.2 ℃) and pH conditions appropriate in Within 1min hydrolyzed casein amino acid equivalent to 1μg phenol (represented by the tyrosine equivalents) amount of enzyme activity of a unit, In U represents. Principle 4 Protease at a certain temperature and pH conditions, the hydrolysis of casein substrate to produce phenol group-containing amino acids (such as. tyrosine, tryptophan), Under alkaline conditions, the forint reagent (Folin) reduction to produce molybdenum and tungsten blue blue is proportional to the depth of amino acid with a phenol content of color. By measuring the absorbance at 680nm to give the amount of enzyme produced phenolic groups of amino acids, and then calculate the protease activity.

5 Reagents and materials

Unless otherwise indicated, the reagents used in the analysis were of analytical grade water are in line with two water GB/T 6682 regulations. 5.1 sodium carbonate solution [c (Na2CO3) = 0.4mol/L] Weigh anhydrous sodium carbonate (Na2CO3) 42.4g, dissolved in water and dilute to 1000mL. 5.2 trichloroacetic acid solution [c (CCl3-COOH) = 0.4mol/L] TCA weighed 65.4g, dissolved in water and dilute to 1000mL. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 28715-2012_English be delivered?

Answer: Upon your order, we will start to translate GB/T 28715-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 28715-2012_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 28715-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.