GB/T 27537-2011 English PDFUS$329.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 27537-2011: Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes Status: Valid
Basic dataStandard ID: GB/T 27537-2011 (GB/T27537-2011)Description (Translated English): Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 14,165 Date of Issue: 2011-11-21 Date of Implementation: 2012-03-01 Quoted Standard: GB/T 6682 Regulation (derived from): Announcement of Newly Approved National Standards No. 18 of 2011 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the type A influenza virus genotyping chip testing procedures. This standard applies to the sample port of all influenza A virus subtypes (H1 ~ H16, N1 ~ N9) screening. GB/T 27537-2011: Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Animal influenza detection. Protocol of DNA microarray examination for influenza A virus subtypes ICS 11.220 B41 National Standards of People's Republic of China Detecting animal influenza type A influenza virus genotyping Microarray procedures Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Chinese Academy of Inspection and Quarantine, the PLA Military Academy of Medical Sciences, China Institute of Veterinary Drug monitoring The police. The main drafters of this standard. Han Xueqing, Liu Bohua, Li, Lin Xiangmei, Wanghui Yu, Liu, Chen Ru, Hou Yihong, Liu Ye Bing Jia Guangle, Wushao Jiang Liang beads, Qin Zhifeng, Ning Yi Bao, such as thin clear, Wang Yiqin. Detecting animal influenza type A influenza virus genotyping Microarray procedures1 ScopeThis standard specifies the type A influenza virus genotyping chip testing procedures. This standard applies to the sample port of all influenza A virus subtypes (H1 ~ H16, N1 ~ N9) screening.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis3 AbbreviationsThe following abbreviations apply to this document. ctr1, ctr2 and ctr3. QC probe 1, 2 and 3 (control1, control2andcontrol3) DEPC. diethyl pyrocarbonate (diethylpyrocarbonate) dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate) PBS. phosphate buffered saline (phosphatebuffersolution) RNA. ribonucleic acid (ribonucleicacid) RT-PCR. reverse transcription-polymerase chain reaction (reversetranscriptionpolymerasechainreaction) SDS. sodium dodecyl sulfate (sodiumdodecylsulfate) SSC. sodium chloride/sodium citrate buffer (sodiumchloride-sodiumcitratebuffer) TAMRA. carboxy tetramethylrhodamine (carboxytetramethylrhodamine)4 Reagents and Instruments4.1 Reagents 4.1.1 test water Distilled water, according to GB/T 6682, three water, except as otherwise provided, the use of biological and chemical reagents were of analytical grade. 4.1.2 Extraction Kit Trizol reagents or commercialization of viral RNA extraction reagent. 4.1.3 A influenza virus multiplex RT-PCR asymmetric synthesis and amplification primers and probes Specific primers and probes (see Appendix A). After the probe and primer synthesis, the use of water-free RNA enzyme (see Appendix B) was dissolved To 50μmol, -20 ℃ stored for use. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 27537-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 27537-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 27537-2011_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 27537-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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