GB/T 27533-2024 English PDF

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GB/T 27533-2024: Diagnostic techniques for canine parvovirus disease
Status: Valid

GB/T 27533: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27533-2024	359	Add to Cart	4 days	Diagnostic techniques for canine parvovirus disease	Valid
GB/T 27533-2011	209	Add to Cart	3 days	Diagnostic techniques for canine parvovirus disease	Valid

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 27530   GB/T 27532   

Basic data

Standard ID: GB/T 27533-2024 (GB/T27533-2024)
Description (Translated English): Diagnostic techniques for canine parvovirus disease
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 18,124
Date of Issue: 2024-12-31
Date of Implementation: 2025-07-01
Older Standard (superseded by this standard): GB/T 27533-2011
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 27533-2024: Diagnostic techniques for canine parvovirus disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220 CCSB41 National Standard of the People's Republic of China Replaces GB/T 27533-2011 Diagnostic technology of canine parvovirus disease Released on 2024-12-31 2025-07-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Table of Contents

Preface III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Biosafety measures 1 6 Clinical Diagnosis 2 7 Sample collection, processing and storage 3 8 Colloidal gold immunochromatography 4 9 Virus isolation and identification 4 10 Hemagglutination and hemagglutination inhibition test (HA/HI) 5 11 PCR 6 12 Fluorescence PCR 8 13 Comprehensive judgment 9 Appendix A (Normative) Preparation of Reagent Solutions 10 Appendix B (Informative) PCR, fluorescent PCR primers and probe sequences 11 Appendix C (Informative) Electrophoresis of Canine Parvovirus PCR Reaction Products 12 Appendix D (Informative) Canine Parvovirus Fluorescence PCR Amplification Curve Figure 13

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 27533-2011 "Diagnostic technology for canine parvovirus disease". Compared with GB/T 27533-2011, except for the structural adjustment, In addition to the integration and editorial changes, the main technical changes are as follows. --- Added abbreviations (see Chapter 4); --- Added biosafety measures (see Chapter 5); --- Added epidemiology (see 6.1); --- Added routine blood test (see 6.3); --- Added colloidal gold immunochromatography (see Chapter 8); ---Added fluorescence PCR (see Chapter 12); --- Added comprehensive judgment (see Chapter 13). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181). This document was drafted by. Qingdao Agricultural University, Fengshi (Qingdao) Marine Technology Co., Ltd., China Animal Health and Epidemiology Center, Qingdao Haihua Biological Group Co., Ltd., Hebei North University, Shandong Academy of Agricultural Sciences, Qingdao Chengyang District Agricultural and Rural Service Center, Changchun Sino Biotechnology Co., Ltd., Shandong Animal Disease Prevention and Control Center, Shanghai Customs Animal and Plant and Food Inspection and Quarantine Technology Center Center, Qingdao Batfei Technology Development Co., Ltd., Chongqing Animal Disease Prevention and Control Center, and Zibo Zhangdian District Animal Husbandry and Fishery Service Center. The main drafters of this document are. Zhang Hongliang, Shan Hu, Ma Qingxia, Su Hong, Li Yifei, Liu Gang, Zhang Ruihua, Xiao Ying, Zhu Weimin, Song Xiaoming, Yang Ruimei, Sun Shengfu, Zhou Fang, Xia Zhenqiang, Xu Chao, Lin Jiaxu, Huang Bing, Yu Yongle, Huang Juan, Qin Zhihua, Luan Weili, Li Mingyi, Li Guimei, Liu Lirong, Li Jian, Ma Fenglong, Zhang Chuanmei, Li Randong, Wen Jianxin, Qin Xiaobing, Ma Jing. The previous versions of this document and the documents it replaces are as follows. ---First published in.2011 as GB/T 27533-2011; ---This is the first revision.

Introduction

An infectious disease of canines and mustelids. The main symptoms of the disease are severe vomiting, hemorrhagic enteritis, significant leukopenia, and myocarditis. There are two types of symptoms, enteritis type and myocarditis type. More than 50% of clinical cases are mixed enteritis and myocarditis type. Animal diseases. Canine parvovirus belongs to the Parvoviridae family and the genus Parvovirus. It is a non-enveloped single-stranded DNA Virus. The nucleotide homology of CPV with parvoviruses such as feline panleukopenia virus (FPLV) and mink enteritis virus (MEV) exceeds 98%. Gene sequence analysis of different isolates found that CPV is prone to produce new mutants through antigenic drift, and there are 4 subtypes. CPV-2, CPV-2a, CPV-2b and CPV-2c. CPV can agglutinate the red blood cells of rhesus monkeys, hamsters, pigs, horses and cats, but cannot agglutinate the red blood cells of humans, cattle, Sheep, rabbit, mouse and chicken red blood cells, so hemagglutination specificity is also one of the reference indicators for identifying the virus. The disease is easily confused with canine distemper and canine coronavirus. It is difficult to confuse it with other diseases such as viral infection and requires a combination of clinical diagnosis and laboratory testing for confirmation. Diagnostic technology of canine parvovirus disease

1 Scope

This document describes the clinical diagnosis of canine parvovirus disease, sample collection, processing and storage, colloidal gold immunochromatography, virus isolation Identification, hemagglutination and hemagglutination inhibition test, PCR, fluorescence PCR and other methods. This document applies to the diagnosis and surveillance of canine parvovirus disease.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB 19489 General requirements for laboratory biosafety NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Abbreviations

The following abbreviations apply to this document. BHQ. black hole quencher CPE. cytopathic effect Ct. cycle threshold FAM.6-carboxyfluorescein

5 Biosafety measures

The sample collection, processing and storage operations shall be carried out in accordance with the provisions of NY/T 541. ......

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