GB/T 27532-2024 English PDF

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GB/T 27532-2024: Diagnostic techniques for canine distemper
Status: Valid

GB/T 27532: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27532-2024	439	Add to Cart	4 days	Diagnostic techniques for canine distemper	Valid
GB/T 27532-2011	229	Add to Cart	3 days	Diagnostic techniques for canine distemper	Valid

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 27530   GB/T 27533   

Basic data

Standard ID: GB/T 27532-2024 (GB/T27532-2024)
Description (Translated English): Diagnostic techniques for canine distemper
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 22,270
Date of Issue: 2024-11-28
Date of Implementation: 2025-06-01
Older Standard (superseded by this standard): GB/T 27532-2011
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 27532-2024: Diagnostic techniques for canine distemper

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220 CCSB41 National Standard of the People's Republic of China Replace GB/T 27532-2011 Canine distemper diagnostic technology Released on 2024-11-28 2025-06-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Table of Contents

Preface III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 1 5 Biosafety measures 2 6 Clinical Diagnosis 2 7 Sample collection, processing and storage 3 8 Colloidal gold immunochromatography 4 9 Virus isolation and identification 4 10 Immunoperoxidase monolayer assay (IPMA) 5 11 Immunoenzyme histochemistry test 7 12 RT-PCR 8 13 Fluorescence RT-PCR 10 14 Comprehensive judgment 12 Appendix A (Normative) Preparation of Reagent Solutions 13 Appendix B (Informative) RT-PCR, Fluorescent RT-PCR Primers and Probe Sequences 15 Appendix C (Informative) Electrophoresis of Canine Distemper Virus RT-PCR Reaction Products 16 Appendix D (Informative) Canine distemper virus fluorescence RT-PCR amplification curve Figure 17

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 27532-2011 "Diagnostic technology for canine distemper". Compared with GB/T 27532-2011, except for the structural adjustment and compilation In addition to logical changes, the main technical changes are as follows. --- Added abbreviations (see Chapter 4); --- Added biosafety measures (see Chapter 5); --- Added epidemiology (see 6.1); --- Added colloidal gold immunochromatography (see Chapter 8); ---Added fluorescent RT-PCR (see Chapter 13); --- Added comprehensive judgment (see Chapter 14). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181). This document was drafted by. Qingdao Agricultural University, Qingdao Haihua Biological Group Co., Ltd., Fengshi (Qingdao) Marine Technology Co., Ltd., China Animal Health and Epidemiology Center, Hebei North University, Shandong Academy of Agricultural Sciences, Qingdao Chengyang District Agricultural and Rural Service Center, Qingdao Batefel Technology Development Co., Ltd., Qingdao Animal Disease Prevention and Control Center, Shanghai Customs Animal and Plant and Food Inspection and Quarantine Technology Center Center, Chongqing Animal Disease Prevention and Control Center. The main drafters of this document are. Shan Hu, Zhang Hongliang, Liu Gang, Song Xiaoming, Ma Qingxia, Wang Yuanyuan, Li Yifei, Zhang Ruihua, Huang Bing, Xiao Ying, Yang Ruimei, Huang Juan, Zhou Fang, Xu Chao, Li Yan, Lin Jiaxu, Li Mingyi, Yu Yongle, Qin Zhihua, Li Jian, Li Guimei, Duan Xiaoxiao, Bing Qizheng, Liu Lirong, Li Randong, Wang Jianlin, Wen Jianxin, Qin Xiaobing, Ma Jing, and Yang Zhen. The previous versions of this document and the documents it replaces are as follows. ---First published in.2011 as GB/T 27532-2011; ---This is the first revision.

Introduction

An acute, highly contagious disease of some raccoons and cats. The body temperature is biphasic in the early stage of the disease, followed by bronchitis, calculus, It is characterized by pneumonia and gastroenteritis. In the later stage, neurological symptoms such as spasms and convulsions can be seen. In some cases, high keratinization of the nose and foot pads may occur. The mortality rate of infected canines is 30% to 80%, and that of ferrets can reach 100%. my country's Ministry of Agriculture and Rural Affairs has listed it as Three types of animal diseases. Canine distemper virus belongs to the Paramyxoviridae family and the Morbillivirus genus. It is a single-stranded RNA There is currently only one serotype of the virus. Canine distemper virus has a wide range of hosts, including Canidae (dogs, foxes, raccoon dogs, wolves, etc.), Mustelidae (minks, ferrets, weasels, etc.), some Procyonidae (raccoons, white-nosed bears, etc.), Animals such as cats (such as raccoons, leopards, and lions) and cats (tigers, leopards, and lions) are susceptible, and young animals are more susceptible than adult animals. The disease is usually more likely to occur 2 to 3 weeks after weaning. Canine distemper virus can cause multi-system diseases in animals, including digestive, respiratory and nervous systems. The clinical symptoms caused by different animal immunity are different. The disease is easily associated with upper respiratory tract infection, canine infectious hepatitis, canine coronavirus infection and other diseases. The disease is confused with each other and needs to be confirmed by combining clinical diagnosis and laboratory tests. Canine distemper diagnostic technology

1 Scope

This document describes the clinical diagnosis of canine distemper, colloidal gold immunochromatography, virus isolation and identification, immunoperoxidase monolayer test, Enzyme histochemistry, RT-PCR, fluorescent RT-PCR and other methods. This document applies to the diagnosis and surveillance of canine distemper.

2 Normative references

The contents of the following documents constitute essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB 19489 General requirements for laboratory biosafety NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Abbreviations

The following abbreviations apply to this document. BHQ. black hole quencher CPE. cytopathic effect Ct. cycle threshold DAB. diaminobenzidine FAM.6-carboxyfluorescein RNA. ribonucleic acid ......

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