GB/T 27536-2011 English PDF

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GB/T 27536-2011: Isolation and identification of swine influenza virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27536-2011	229	Add to Cart	3 days	Isolation and identification of swine influenza virus	Valid

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Basic data

Standard ID: GB/T 27536-2011 (GB/T27536-2011)
Description (Translated English): Isolation and identification of swine influenza virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,161
Date of Issue: 2011-11-21
Date of Implementation: 2012-03-01
Regulation (derived from): Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the swine flu virus isolation and identification methods. This standard applies to various subtypes of swine flu pathogen isolation and identification.

GB/T 27536-2011: Isolation and identification of swine influenza virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Isolation and identification of swine influenza virus ICS 11.220 B41 National Standards of People's Republic of China Swine flu virus isolation and identification methods Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The main drafters of this standard. Qiaozhuan Ling, Yanghuan Liang, Chen Yan, Xin Xiaoguang, Chen Hualan. Swine flu virus isolation and identification methods

1 Scope

This standard specifies the swine flu virus isolation and identification methods. This standard applies to various subtypes of swine flu pathogen isolation and identification. 2 test materials, reagents 2.1 scissors, tweezers. 2.2 1mL syringe. 2.3 single-channel and multi-channel micro pipettes, tips. 2.4 9 to 11-day-old SPF chicken embryos. 2.5 canine kidney cells (MDCK). 2.6 6-well culture plates. 2.7 DMEM medium. 2.8 HEPES buffer (1mol/L stock solution). 2.9 FBS. 2.10 TPCK- trypsin (TPCK trypsin after treatment). 2.11 0.01mol/LpH7.0 ~ 7.4 phosphate buffered saline (PBS) (see Appendix A A.1). 2.12 phosphate buffer (pH5.9) (see Appendix A, A.5). 2.13 0.85% saline (see Appendix A, A.2). 2.14 Aschoff (Alsevers) solution (see Appendix A A.3). 2.15 0.5% red blood cell suspension (see Appendix A A.4). 2.16 fetuin (see Appendix A, A.6). 2.17 over iodate (see Appendix A, A.7). 2.18 arsenite (see Appendix A in A.8). 2.19 thiobarbituric acid (see Appendix A, A.9). 2.20 sterile distilled or deionized water.

3 Instrument

3.1 Biological safety cabinets (cabinet vacuum with a HEPA filter to avoid contamination of the cell culture and the protection of operator safety). 3.2 incubator. 3.3 CO2 incubator. 3.4 desktop high-speed refrigerated centrifuge. Water bath heater 3.5 37 ℃. 3.6 boiling pot. 3.7 cotton swabs (medical cotton). 3.8 mill. ......

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