GB/T 25221-2010 English PDF

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GB/T 25221-2010: Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 25221-2010	209	Add to Cart	3 days	Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography	Valid

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Basic data

Standard ID: GB/T 25221-2010 (GB/T25221-2010)
Description (Translated English): Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X10
Classification of International Standard: 67.040
Word Count Estimation: 9,959
Date of Issue: 2010-09-26
Date of Implementation: 2011-03-01
Quoted Standard: GB 5491; GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2010 No.6 (Total No.161)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the terms and definitions of food in the determination of ergosterol normal phase high performance liquid chromatography, principle, reagents and materials, instruments and equipment, for sampling, sample preparation, steps, result of the calculation and representation, repeatability requirements. This standard applies to rice, determination of ergosterol in grain corn, wheat. The method detection resistor is 0. 05 mg/kg.

GB/T 25221-2010: Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils.Determination of ergosterol in grain. Normal phase high performance liquid chromatography ICS 67.040 X10 National Standards of People's Republic of China Determination of ergosterol in foodstuffs by grain and oil Normal Phase High Performance Liquid Chromatography 2010-09-26 release 2011-03-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People 's Republic of China China National Standardization Management Committee released

Foreword

Appendix A to this standard is an informative appendix. This standard is proposed by the National Grain Bureau. This standard by the National Grain Standardization Technical Committee. The standard responsible for the drafting unit. the State Grain Reserve Bureau of Chengdu Grain Storage Science Research Institute, Liaoning Province, grain and oil inspection and monitoring. The main drafters of this standard. He Xuechao, Feng Yongjian, Jiang Tao, Zhong Yu, Xiao Xuebin, Wang Deqian, Xiong Shengwei, Qiao Lina. Determination of ergosterol in foodstuffs by grain and oil Normal Phase High Performance Liquid Chromatography

1 Scope

This standard specifies the terms and definitions of ergosterol in food by normal phase high performance liquid chromatography, principles, reagents and materials, instruments and Equipment, sampling, sample preparation, operating procedures, calculation and presentation of results, repeatability requirements. This standard applies to rice, wheat, corn and other food ergosterol determination. The detection limit of this method is 0.05mg/kg.

2 normative reference documents

The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all (Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard. GB 5491 grain, oilseed inspection, sampling method GB/T 6682 Analytical laboratory water specifications and test methods

3 terms and definitions

The following terms and definitions apply to this standard. 3.1 Ergosterol ergosterol Derived from the infection of food fungi (spores or mycelia) produced free ergosterol and ergosterol phosphate material, is the fungal cells Normal metabolites, and fungal cell membrane is one of the components.

4 principle

After saponification of the sample, extraction of ergosterol with n-hexane, the extract was dehydrated, concentrated and silica gel column separation, with high performance liquid chromatography violet External detector at 282nm at the detection, external standard quantitative.

5 reagents and materials

Unless otherwise specified, all reagents are of analytical grade, and the test water shall comply with the requirements of Grade 3 water in GB/T 6682, GB/T 6682 requirements for primary water requirements. 5.1 n-hexane. pure chromatography. 5.2 isopropyl alcohol. pure chromatography. 5.3 n-hexane. analytical grade for extraction. 5.4 Potassium hydroxide. 5.5 50% potassium hydroxide solution. Weigh 10 g of potassium hydroxide (5.4) and add 10 mL of water to dissolve. 5.6 Methanol. 5.7 2,6-di-tert-butyl-p-cresol (BHT). 5.8 Vitamin C (ascorbic acid). 5.9 Coke gallic acid. ......

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