GB/T 23881-2009 English PDFUS$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 23881-2009: Determination of feed cellulase activity -- Filter paper assay method Status: Valid
Basic dataStandard ID: GB/T 23881-2009 (GB/T23881-2009)Description (Translated English): Determination of feed cellulase activity -- Filter paper assay method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 8,883 Date of Issue: 2009-05-26 Date of Implementation: 2009-10-01 Regulation (derived from): PRC National Standard Approval Announcement 2009 No.7 (Total No.147) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the filter paper as a substrate, with sugar feeding cellulase activity colorimetric assay method. This standard applies to the determination of feed cellulase activity. Quantitative detection limit of 0. 02 U/mL. GB/T 23881-2009: Determination of feed cellulase activity -- Filter paper assay method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of feed cellulase activity. Filter paper assay method ICS 65.120 B46 National Standards of People's Republic of China Fodder cellulase activity measurement Filter paper method Posted 2009-05-26 2009-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard by the National Standardization Technical Committee Feed Industry and focal points. This standard was drafted. National Feed Quality Supervision and Inspection Center (Wuhan). The main drafters of this standard. Hefeng Qin, Yang Lin, money Chuang, Liu Xiaomin, Qu Liwen Huang Ting, Yanke Liang. Fodder cellulase activity measurement Filter paper method1 ScopeThis standard specifies the filter paper as a substrate, with a reducing sugar colorimetric assay method of feed cellulase activity. This standard applies to the determination of feed cellulase activity, the quantitative detection limit of 0.02U/mL.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Test water specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation3 Terms and DefinitionsThe following terms and definitions apply to this standard. 3.1 At 37 ℃, pH5.5, for 60min the reaction conditions, the amount of enzyme degradation of paper per minute required to release glucose 1μmol, defined as A filter paper cellulase activity units to U represent. Principle 4 Cellobiose, glucose and other reducing sugars can filter the alkaline conditions generated by enzymatic hydrolysis of 3,5-dinitrosalicylic acid reduction, Generate reddish brown amino compounds have a maximum absorption at 540nm wavelength, the amount within a certain range of the enzyme produced by the reaction of reducing sugars Proportional to the absorbance of the solution.5 Reagents and materialsThe agents of the criteria used were of analytical grade water are in line with two water GB/T 6682 stipulated. 5.1 potassium sodium tartrate (C4H4KNaO6 · 4H2O). 5.2 phenol. 5.3 sodium sulfite. 5.4 Sodium hydroxide solution (200g/L). Weigh sodium hydroxide 20.0g, add water, 100mL dissolve. 5.5 citric acid solution (0.1mol/L). Weigh citric acid (C6H8O7 · H2O) 2.10g, dissolved in water and dilute to 100mL. 5.6 sodium citrate solution (0.1mol/L). Weigh sodium citrate (Na3C6H5O7 · 2H2O) 2.94g, dissolved in water and dilute to 100mL. 5.7 citrate buffer (0.05mol/L, pH5.5). Weigh citric acid (C6H8O7 · H2O) 10.5g, was added sodium hydroxide 5.0g, Plus 800mL dissolved in water, adjusted with citric acid solution (5.5) or sodium citrate solution (5.6) pH to 5.5, and then water to the 1000mL. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 23881-2009_English be delivered?Answer: Upon your order, we will start to translate GB/T 23881-2009_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 23881-2009_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 23881-2009_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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