GB/T 23874-2009 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 23874-2009: Determination of xylanase activity in feed additives -- Spectrophotometric method Status: Valid
Basic dataStandard ID: GB/T 23874-2009 (GB/T23874-2009)Description (Translated English): Determination of xylanase activity in feed additives -- Spectrophotometric method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 10,180 Date of Issue: 2009-05-26 Date of Implementation: 2009-10-01 Regulation (derived from): PRC National Standard Approval Announcement 2009 No.7 (Total No.147) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the activity measured by spectrophotometry feed additive xylanase. This standard applies to xylanase feed additive products, the minimum detectable amount was 10. 0 U/g. GB/T 23874-2009: Determination of xylanase activity in feed additives -- Spectrophotometric method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of xylanase activity in feed additives. Spectrophotometric method ICS 65.120 B46 National Standards of People's Republic of China Determination of feed additives xylanase activity of Posted 2009-05-26 2009-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordAppendix A of this standard is an informative annex. This standard by the National Standardization Technical Committee Feed Industry and focal points. This standard by the Chinese Ministry of Agriculture Feed Industry Center, Wuhan Xinhua Yang Biotechnology Co., Ltd., Guangdong overflow Dolly biotechnology shares Ltd. is responsible for drafting. The main drafters of this standard. Lu Wenqing, Cao Yunhe, Liu Xinghai, Zhanzhi Chun, Liu Ya force, Chen Qinghua. Determination of feed additives xylanase activity of1 ScopeThis standard specifies the viability assay by spectrophotometry feed additive xylanase. This standard applies to xylanase feed additive products, the minimum detectable amount of 10.0U/g.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 Xylanase can xylan degrading into oligosaccharides and monosaccharides. Reducing oligosaccharides and monosaccharides in a boiling water bath conditions with 3,5-dinitro Salicylic acid (DNS) reagent color reaction. The amount of reducing sugars and the reaction liquid color depth enzyme produced is proportional to the generation of reducing sugars Volume and vitality and the reaction mixture is proportional to xylanase. Thus, the color intensity of the reaction solution by colorimetric spectroscopic measurement, the reaction solution can be calculated Xylanase vitality. At 37 ℃, pH 5.50 under the conditions of xylan per minute from a solution at a concentration of 5mg/mL reduction in degradation release 1μmol Sugar amount of enzyme required for the enzyme activity unit U.4 Reagents and solutionsUnless otherwise specified, the reagents used were of analytical grade water are in line with two water GB/T 6682 stipulated. Weigh sodium hydroxide 20.0g. Dissolved in water, dilute to 100mL. Draw glacial acetic acid 0.60mL. Dissolved in water, dilute to 100mL. Weigh sodium acetate trihydrate 1.36g. Dissolved in water, dilute to 100mL. Weigh sodium acetate trihydrate 23.14g, glacial acetic acid was added 1.70mL. Add water to dissolve, dilute to 2000mL. PH of the solution was measured. If the deviation from pH 5.50, and then a solution of acetic acid (4.2) or sodium acetate (4.3) was adjusted to 5.50. Weigh anhydrous xylose 1.000g, plus acetic acid - sodium acetate buffer solution (4.4) to dissolve the volume to 100mL. 1) SigmaX0627 is a trade name, this information is given for the convenience of users of this standard does not imply endorsement of the product. If other products Can have the same effect, you can use these equivalent products. Weigh xylan (SigmaX06271)) 1.00g, was added 0.32g of sodium hydroxide, then 90mL of water was added, with magnetic stirring while slowly ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 23874-2009_English be delivered?Answer: Upon your order, we will start to translate GB/T 23874-2009_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 23874-2009_English with my colleagues?Answer: Yes. 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