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GB/T 23877-2009 English PDF

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GB/T 23877-2009: Determination of citric acid, fumaric acid and lactic acid in feed acidifier -- High performance liquid chromatography
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 23877-2009189 Add to Cart 3 days Determination of citric acid, fumaric acid and lactic acid in feed acidifier -- High performance liquid chromatography Valid

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Basic data

Standard ID: GB/T 23877-2009 (GB/T23877-2009)
Description (Translated English): Determination of citric acid, fumaric acid and lactic acid in feed acidifier -- High performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 8,841
Date of Issue: 2009-05-26
Date of Implementation: 2009-10-01
Regulation (derived from): PRC National Standard Approval Announcement 2009 No.7 (Total No.147)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the determination of citric acid in the feed acidifiers, fumaric acid and lactic acid content by HPLC. This standard applies to feed acidifier citric acid, determination of fumaric acid and lactic acid. The limit of quantification of the method are: citric acid 800 mg/kg, fumarate 8. 0 mg/kg, acid 800 mg/kg.

GB/T 23877-2009: Determination of citric acid, fumaric acid and lactic acid in feed acidifier -- High performance liquid chromatography


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of citric acid, fumaric acid and lactic acid in feed acidifier. High performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Feed acidifiers are citric acid, fumaric acid and Lactic acid by HPLC Posted 2009-05-26 2009-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is a normative appendix, Appendix B is an informative annex. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points. This standard was drafted. Shanghai Feed Quality Supervision, Inspection Station, the Shanghai Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Institute The study. The main drafters of this standard. Zhao Zhihui, Huang Zhiying, Gu race red, T. (MONTHLY) Lin Miao, Bai Bing, Ray Ping, Mao Feng Xi. Feed acidifiers are citric acid, fumaric acid and Lactic acid by HPLC

1 Scope

This standard specifies the determination of the feed acidifiers citric acid, fumaric acid and lactic acid content by HPLC. This standard applies to feed acidifiers are citric acid, fumaric acid, lactic acid levels measured. Quantification limit of the method is. citrate 800mg/kg, fumaric acid 8.0mg/kg, lactic acid 800mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Preparation of GB/T 601 chemical reagent standard titration solution Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples Principle 3 Sample citric acid, fumaric acid and lactic acid with water after ultrasonic extraction, diluted to the appropriate concentration was measured by high performance liquid chromatography, outside Calculate the content of citric acid, fumaric acid and lactic standard method.

4 Reagents and materials

Unless otherwise indicated, the methods used were of analytical grade reagents and water to comply with GB/T 6682 Category 2 water. 4.1 acetonitrile. chromatography. 4.2 Phosphate. chromatography. 4.3 0.1% phosphoric acid solution. Imbibe 1.00mL phosphoric acid (4.2), in line with GB/T 6682 provided an dissolved in water and dilute to 1000mL. 4.4 citrate standards. purity ≥99.5%. 4.5 fumarate standards. purity ≥99.5%. 4.6 Citric acid standard stock solution. Weigh accurately citric standard 0.5025g in 50mL volumetric flask, dissolved in water and dilute to the mark degree. This concentration of 10.0mg/mL, at 4 ℃ storage, shelf life of three months. 4.7 fumarate stock standard solution. Accurately weigh 0.1000g fumaric standard in 100mL volumetric flask, dissolved in water and dilute to the mark degree. This concentration of 1.0mg/mL, at 4 ℃ storage, shelf life of three months. 4.8 lactic acid standard stock solution. preparation and calibration methods, see Appendix A. 4.9 mixed standard stock solution. Take 10.0mL citric acid standard stock solution (4.6), 1.0mL fumaric acid standard stock solution (4.7) and 10.0mL Lactic acid standard stock solution (4.8) in 50mL volumetric flask with water to volume, and mix. At 4 ℃ storage, shelf life of one month. 4.10 mixed standard working solution. Imbibe 0.10mL, 0.25mL, 0.50mL, 1.00mL, 2.50mL, 5.00mL, 10.00mL mixed standard stock solution (4.9) in 10.0mL volumetric flask with water to volume, and mix. This is the working standard solutions, Now with the current.
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