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GB/T 21542-2025 English PDF

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US$209.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 21542-2025: Determination of enramycin in feeds - Liquid chromatography-tandem mass spectrometry
Status: Valid

GB/T 21542: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21542-2025	209	Add to Cart	3 days	Determination of enramycin in feeds - Liquid chromatography-tandem mass spectrometry	Valid
GB/T 21542-2008	189	Add to Cart	3 days	Determination of enramycin in feeds -- Microbiological method	Valid

Similar standards

GB 22549 GB 22548 GB/T 23180 GB/T 17812 GB/T 21543

Basic data

Standard ID: GB/T 21542-2025 (GB/T21542-2025)
Description (Translated English): Determination of enramycin in feeds - Liquid chromatography-tandem mass spectrometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 10,194
Date of Issue: 2025-06-30
Date of Implementation: 2026-01-01
Older Standard (superseded by this standard): GB/T 21542-2008
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 21542-2025: Determination of enramycin in feeds - Liquid chromatography-tandem mass spectrometry

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT21542-2025
National Standard of the People's Republic of China ICS 65.120CCS B 46 Determination of Enramycin in Feed Liquid chromatography-tandem mass spectrometry Determination of enramycin in feeds- Liquid chromatography⁃tandem mass spectrometry Released on June 30, 2025 2026-01-01 implementation State Administration for Market Regulation The National Standardization Administration issued Replaces GB/T 21542-2008

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting is required. This document replaces GB/T 21542-2008 "Determination of Enramycin in Feed - Microbiological Method" and is in compliance with GB/T 21542-2008. In addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope has been changed (see Chapter 1 of the.2008 edition); b) Microbiological methods (see Chapters 3 to 10 and Appendix A of the.2008 edition) have been deleted; c) Liquid chromatography-tandem mass spectrometry has been added (see Chapters 4 to 10). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76). This document was drafted by. Shanghai Animal Disease Prevention and Control Center, Tongwei Agricultural Development Co., Ltd., Henan Provincial Agricultural, Animal and Aquatic Products Inspection Technology Research Institute. The main drafters of this document are. Yan Feng, Cao Ying, Zhang Fengping, Peng Li, Zhang Jing, Shang Jun, Chang Shouhai, Huang Shixin, Wu Yushan, Pan Juan, Zhang Jingjing, Huang Jiaying, Nie Xiaohong, Wu Jianping, Lu Jiawen, Dong Peng, Li Huawei, Zhang Haoran, Xu Ting, and Zhang Yifei. The previous versions of this document and the documents it replaces are as follows. --First published in.2008 as GB/T 21542-2008; --This is the first revision. Determination of Enramycin in Feed Liquid chromatography-tandem mass spectrometry

1 Scope

This document describes a method for the determination of enramycin in feed by liquid chromatography-tandem mass spectrometry. This document applies to Enramyces in compound feeds, concentrated feeds, concentrate supplements, additive premixes and mixed feed additives Determination of enramycin (calculated as the sum of enramycin A and enramycin B). In this document, the detection limits of enramycin A and enramycin B are both 0.05 mg/kg, and the quantification limits are both 0.10 mg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Principles

The analytes in the sample were extracted with a methanolic hydrochloric acid solution or a methanolic formic acid solution, cleaned up with a solid phase extraction column, and measured by liquid chromatography-tandem mass spectrometry. Quantification was performed by matrix-matched external standard method.

5 Reagents or materials

Unless otherwise specified, only analytical grade reagents were used. 5.1 Water. GB/T 6682, Grade 1. 5.2 Methanol. chromatographic grade. 5.3 Formic acid. chromatographic grade. 5.4 Ethyl acetate. 5.5 Hydrochloric acid solution (0.1 mol/L). Take 4.2 mL of hydrochloric acid, dilute to 500 mL with water, and mix thoroughly. 5.6 Hydrochloric acid methanol solution. Pipette 55 mL of methanol and 45 mL of hydrochloric acid solution (5.5) and mix well. 5.7 2% formic acid methanol solution. Pipette 2 mL of formic acid, dilute to 100 mL with methanol, and mix thoroughly. 5.8 5% ammonia solution. Pipette 5 mL of ammonia solution, dilute to 100 mL with water, and mix thoroughly. 5.9 Methanol-ammonia solution. Pipette 20 mL of methanol and 80 mL of 5% ammonia water (5.8) and mix well. 5.10 1% formic acid in ethyl acetate. Pipette 1 mL of formic acid, dilute to 100 mL with ethyl acetate, and mix by ultrasonication. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 21542-2025_English be delivered?

Answer: Upon your order, we will start to translate GB/T 21542-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 21542-2025_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 21542-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 21542-2025?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 21542-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.