Path: Home > GB/T > Page739 > GB/T 18654.13-2026

Price & Delivery

US$219.00 · In stock · Download in 9 seconds
GB/T 18654.13-2026: Inspection of germplasm for fishes - Part 13: Test method of isozyme electrophoresis
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Status: Valid

Std ID	Version	USD	Buy	Deliver [PDF] in	Title (Description)
GB/T 18654.13-2026	English	219	Add to Cart	3 days [Need to translate]	Inspection of germplasm for fishes - Part 13: Test method of isozyme electrophoresis
GB/T 18654.13-2008	English	189	Add to Cart	3 days [Need to translate]	Inspection of germplasm for cultured fishes -- Part 13: Analysis of isozyme electrophoresis

GB/T 18654.13-2026: Inspection of germplasm for fishes - Part 13: Test method of isozyme electrophoresis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.150 CCSB50 National Standards of the People's Republic of China Replaces GB/T 18654.13-2008 Fish germplasm testing Part 13.Isozyme Electrophoresis Analysis Published on 2026-02-27 Implemented on 2026-09-01 State Administration for Market Regulation The State Administration for Standardization issued a statement.

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents". Drafting. This document is Part 13 of GB/T 18654, "Fish Germplasm Inspection". GB/T 18654 has already published the following parts. ---Part 1.Inspection Rules; ---Part 2.Sampling Methods; ---Part 3.Characteristic Determination; ---Part 4.Measurement of Age and Growth; ---Part 5.Dietary Analysis; ---Part 6.Determination of reproductive performance; ---Part 7.Ecological Characteristics Analysis; ---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxia Point; ---Part 9.Meat yield determination; ---Part 10.Determination of muscle nutrient composition; ---Part 12.Karyotype Analysis; ---Part 13.Isozyme Electrophoresis Analysis; ---Part 14.Determination of DNA content. This document replaces GB/T 18654.13-2008 "Geological Examination of Cultured Fish - Part 13.Isozyme Electrophoresis Analysis", and is consistent with... Compared with GB/T 18654.13-2008, apart from structural adjustments and editorial changes, the main technical changes are as follows. a) The principle has been changed (see Chapter 4, Chapter 3 of the.2008 edition); b) Reagents and materials have been changed (see Chapter 5, Chapter 4 of the.2008 edition); c) The instruments and equipment were changed (see Chapter 6, Chapter 5 of the.2008 edition); d) The sampling method was changed (see Chapter 7, Chapter 6 in the.2008 edition); e) Experimental procedures and data processing have been changed (see Chapters 8 and 9, Chapter 7 in the.2008 edition); f) The result determination has been removed (see Chapter 8 of the.2008 edition). Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Fisheries Standardization (SAC/TC156). This document was drafted by. Shanghai Ocean University, Yangtze River Fisheries Research Institute of Chinese Academy of Fishery Sciences, and East China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences. Fisheries Research Institute, Yunnan Provincial Fisheries Research Institute, Heilongjiang Fisheries Research Institute of Chinese Academy of Fishery Sciences. The main drafters of this document are. Zhao Jinliang, Tang Shoujie, Zhao Yan, Liu Yongtao, Zhou Jianguang, Zhang Lin, Zhang Tao, Zhou Ruiqiong, Li Yan, and Yao Zongli. Lai Qifang, Miao Xiangjun, Li Datian, Fan Peng, Sun Yunchao. The release history of this document and the document it replaces is as follows. ---First published in.2008 as GB/T 18654.13-2008; ---This is the first revision.

Introduction

Aquatic germplasm resources are an important material basis for fisheries production activities. The "Genomic Testing of Cultured Fish" standard includes sections on fish germplasm testing. This is a fundamental and universal standard. Since its implementation, the standard has played an important role in fish resource surveys, conservation, propagation, and utilization. To further... To better serve the needs of fisheries development and testing, the name of "Cultivated Fish Germplasm Inspection" has been changed to "Fish Germplasm Inspection," and some parts of the standard have been revised accordingly. The content has been revised. GB/T 18654 is proposed to consist of the following parts. ---Part 1.Inspection Rules. This section describes the classification, inspection items, judgment rules, and re-inspection rules for fish germplasm testing. ---Part 2.Sampling Methods. The purpose is to describe the reagents and equipment, sampling rules, and sampling procedures used in fish germplasm testing. Order, sample size, and sample preservation. ---Part 3.Trait Determination. The purpose is to describe the reagents and materials, instruments and equipment, sample preparation, and experiments used in the determination of fish traits. Steps and data processing. ---Part 4.Determination of Age and Growth. The purpose is to describe the reagents, materials, instruments, and equipment used in determining the age and growth of fish. Samples, test procedures, and data processing. ---Part 5.Feeding Analysis. The purpose is to describe the reagents and materials, instruments and equipment, samples, experimental procedures, and methods used in fish feeding analysis. Data processing. ---Part 6.Determination of Reproductive Performance. The purpose is to describe the reagents, materials, instruments, and equipment used in determining egg-bearing and spawning rates in fish. Samples, test procedures, and data processing. ---Part 7.Ecological Characteristics Analysis. This section describes the reagents and instruments used to analyze the ecological characteristics (temperature, salinity tolerance) of fish. Equipment, samples, test procedures, and data processing. ---Part 8.Determination of Oxygen Consumption Rate and Critical Asphyxiation Point in Fish. The purpose is to describe the reagents used in determining oxygen consumption rate and critical asphyxiation point in fish. Materials, instruments and equipment, samples, test procedures and data processing. ---Part 9.Determination of Flesh Percentage. The purpose is to describe the reagents and materials, instruments and equipment, samples, and experimental procedures used in the determination of flesh percentage in fish. Steps and data processing. ---Part 10.Determination of Muscle Nutritional Components. The purpose is to describe the nutritional components of fish muscle (moisture, ash, protein, amino acids). Reagents and materials, instruments and equipment, samples, test procedures and data processing for the determination of acids, fats and fatty acids. ---Part 12.Karyotype Analysis. The purpose is to describe the principles, reagents and materials, and instruments used in fish karyotype analysis. Preparation, samples, test procedures and data processing. ---Part 13.Isozyme Electrophoresis Analysis. The purpose is to describe the reagents, materials, instruments, and samples used in isozyme electrophoresis analysis of fish. Product, testing procedures and data processing. ---Part 14.Determination of DNA Content. The purpose is to describe the reagents, materials, instruments, and equipment used in the determination of DNA content in fish. Samples, test procedures, and data processing. Fish germplasm testing Part 13.Isozyme Electrophoresis Analysis

1 Scope

This document describes the principles, reagents and materials, instruments and equipment, samples, and experimental procedures for the analysis of fish isoenzymes using polyacrylamide gel electrophoresis. And data processing. This document applies to isozyme electrophoresis analysis of fish.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included. For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies. This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 18654.2 Fish germplasm testing – Part 2.Sampling methods

3 Terms and Definitions

This document does not contain any terms or definitions that need to be defined. 4.Principles Isoenzymes are a group of enzymes in fish that catalyze the same reaction but differ in molecular structure and physicochemical properties. They are classified according to their electrical charge. Due to differences in charge, molecular weight, and structure, isoenzyme components exhibit different migration characteristics in polyacrylamide gel media under the influence of an electric field. The enzyme bands, after catalytic reaction and staining, exhibit different numbers of enzyme bands, serving as biochemical genetic characteristics of fish germplasm. 5.Reagents and Materials Warning---Acrylamide, N,N'-methylenebisacrylamide or methylenebisacrylamide, tetramethylethylenediamine, phenazine methyl ester sulfate, Nitrotetrazole blue chloride, α-naphthyl acetate, and β-naphthyl acetate are toxic chemicals. Respiratory and skin protection measures should be taken when handling them. Wash your hands after using it. Unless otherwise specified, all reagents were of analytical grade. The water used in the tests was Grade I water conforming to GB/T 6682. 5.1 Reagents 5.1.1 Glacial acetic acid (CH3COOH). 5.1.2 Ethanol (CH3CH2OH). 5.1.3 Glycerol (C3H8O3). 5.1.4 Sodium hydroxide (NaOH). 5.1.5 Nitrotetrazolium chloride (NBT). Store at 4°C. ...