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GB/T 18397-2014 PDF English

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GB/T 18397-2014: Determination of pantothenic acid in compound premix -- High-pressure liquid chromatography
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GB/T 18397: Historical versions

Std IDVersionUSDBuyDeliver [PDF] inTitle (Description)
GB/T 18397-2014English169 Add to Cart 3 days [Need to translate] Determination of pantothenic acid in compound premix -- High-pressure liquid chromatography
GB/T 18397-2001English199 Add to Cart 2 days [Need to translate] Determination of pantothenic acid in compound premix -- High-pressure liquid chromatography

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Basic data

Standard ID GB/T 18397-2014 (GB/T18397-2014)
Description (Translated English) Determination of pantothenic acid in compound premix -- High-pressure liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 7,783
Date of Issue 7/8/2014
Date of Implementation 1/10/2015
Older Standard (superseded by this standard) GB/T 18397-2001
Quoted Standard GB/T 6682; GB/T 14699.1
Regulation (derived from) 2014 National Standards Bulletin No. 18
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method for determining premix pantothenic acid content by high performance liquid chromatography. This standard applies to composite pre-mixed feed, feed Determination of pantothenic acid vitamin pre-mix. The standard measureme

GB/T 18397-2014: Determination of pantothenic acid in compound premix -- High-pressure liquid chromatography




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Determination of pantothenic acid in compound premix - High-pressure liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Replacing GB/T 18397-2001 Determination of pantothenic acid premix High Performance Liquid Chromatography Issued on. 2014-07-08 2015-01-10 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces GB/T 18397-2001 "compound premix pantothenic acid by HPLC." This standard compared with GB/T 18397-2001 main changes are as follows. --- Standard name from "composite premix pantothenic acid HPLC" to "premix pantothenic acid test Given HPLC "; --- Range removed "also applies to the determination of pantothenic acid feed concentration"; the "measuring range per kilogram of pantothenic acid contained in the sample 50mg or more "to" this standard assay of pantothenic acid detection limit of 5mg/kg, the limit of quantification of 20mg/kg "; --- Sample extraction agent without EDTA, the direct use of the mobile phase was extracted; --- The mobile phase was replaced with a mixture of acetonitrile and phosphate buffer with 0.05% phosphoric acid solution; --- Calculation results with external standard method multi-point calibration or single-point calibration instead of the original "single-point calibration." This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard was drafted. Jiangxi veterinary feed Control, Tongwei Company. The main drafters of this standard. Fu Jinhua, Yangfa tree, rainbow text, Rao Hui, Liuyao Min, Feng-Ping, Fan Jing, Zhao Yan. This standard replaces the standards previously issued as follows. --- GB/T 18397-2001. Determination of pantothenic acid premix High Performance Liquid Chromatography

1 Scope

This standard specifies the method for determining premix pantothenic acid content by high performance liquid chromatography. This standard applies to composite pre-mixed feed, feed Determination of pantothenic acid vitamin pre-mix. The standard measurement of pantothenic acid detection limit of 5mg/kg, the limit of quantification of 20mg/kg.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples Principle 3 Pantothenic acid in the sample with acetonitrile phosphoric acid extraction, centrifugation, filtration, separation C18 column, UV detector and external standard method Quantified.

4 Reagents and solutions

Except where noted, this standard reagents used were of analytical grade, water line with two water GB/T 6682 stipulated. 4.1 phosphoric acid. the content of ≥85%. 4.2 acetonitrile. chromatography. 4.3 0.05% phosphoric acid solution. 0.5mL phosphate (4.1) was added to the flask, and dilute to 1000mL. 4.4 D- pantothenic acid (calcium pantothenate or D-) standard solution. 4.4.1 Standard stock solution. Weigh D- D- pantothenic acid or calcium pantothenate standard pure amount of goods from the purity of more than 99.0% in 100mL flask With mobile phase dissolved constant volume, so that the concentration of the standard stock solution containing pantothenic acid is 1000μg/mL. This standard solution can be stored at 4 ℃ three Months. Pantothenic acid concentration = Concentration D- calcium pantothenate × 0.920. 4.4.2 standard solution. Imbibe were stock standard solution (4.4.1) 0.5mL, 1.0mL, 2.0mL, 5.0mL, 10.0mL in 100mL flask, with the mobile phase to volume, to give concentrations of 5.0μg/mL, 10.0μg/mL, 20.0μg/mL, 50.0μg/mL, pantothenic standard 100.0μg/mL working solution. Using now. 5. Apparatus 5.1 Analysis of balance. a sense of the amount of 0.0001g. 5.2 centrifuge. speed 3000r/min. 5.3 shaker. ...

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