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GB/T 16125: Historical versions
| Std ID | Version | USD | Buy | Deliver [PDF] in | Title (Description) |
| GB/T 16125-2012 | English | 209 |
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Method for acute toxicity test of daphnia magna straus
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| GB/T 16125-1995 | English | 239 |
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Standard methods for bioassay of daphnia magna straus
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Basic data
| Standard ID | GB/T 16125-2012 (GB/T16125-2012) |
| Description (Translated English) | Method for acute toxicity test of daphnia magna straus |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | C51 |
| Classification of International Standard | 13.060 |
| Word Count Estimation | 9,977 |
| Older Standard (superseded by this standard) | GB/T 16125-1995 |
| Quoted Standard | GB/T 6682; GB 12997; GB 12998; GB 12999 |
| Adopted Standard | ISO 6341-1996, NEQ |
| Regulation (derived from) | National Standards Bulletin No. 31 of 2012 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard provides a large scratch acute toxicity test method. This standard applies to evaluate the toxicity of soluble chemicals, industrial wastewater and solid waste leachate comprehensive toxicity, wastewater treatment effect, surface water, grou |
GB/T 16125-2012: Method for acute toxicity test of daphnia magna straus
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ICS13.060
C51
National Standards of People's Republic of China
Replacing GB/T 16125-1995
Daphnia magna acute toxicity test method
(ISO 6341-1996Waterquality-Determinationoftheinhibition
ofthemobilityofDaphniamagnaStraus (Cladocera, Crustacea) -
Acutetoxicitytest, NEQ)
Issued on. 2013-05-01 2012-11-20 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This standard rule according to GB/T 1.1-2009 given draft.
This standard replaces GB/T 16125-1995 "Daphnia magna test standard method", compared with the GB/T 16125-1995 main technical change
Of the following.
--- The original standard name "Daphnia magna test standard method" to "Daphnia magna acute toxicity test methods";
--- Original standard subject matter and scope of application to modify the scope and removes Daphnia growth and reproduction of experimental methods (chronic effects);
--- According to GB/T 1.1-2009 restructured, supplemented with "normative references", "Terms and Definitions";
--- The standard 4.2 adds definition and formulation methods standard dilution water;
--- Increased 6.1 This standard sample collection and preservation methods;
--- The standard 7.1 increase in the content and the limits of experimental methods;
--- This standard adds quality control and quality assurance of content and methods;
--- Original Standard Appendix A Appendix A to adjust "culture propagation method Daphnia magna" and Appendix B "Scenedesmus obliquus culture techniques."
The use of standard reference redrafted ISO 6341.1996 "Quality - Daphnia magna (Crustacea, Cladocera) activity inhibition assay" establishment,
And ISO 6341.1996 is non-equivalent.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard was drafted. Chinese Center for Disease Control Environmental Health and Related Product Safety.
The main drafters of this standard. Liu Fan, Pan Lijun, high Shirong, Wang Jun together.
Daphnia magna acute toxicity test method
1 Scope
This standard specifies the Daphnia magna acute toxicity test method.
This standard applies to the evaluation of the toxicity of soluble chemicals, the combined toxicity of industrial wastewater and solid waste leachate, wastewater treatment
Effects, toxicity of surface water, groundwater and water deposits.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 6682 analytical laboratory use specifications and test methods
Technical regulation for design GB 12997 water sampling program
GB 12998 technical guidance Water Sampling
Conservation and management of technical regulations GB 12999 water sampling sample
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
EC50 medianeffectiveconcentration
EC50
In the 24h or 48h 50% within the experimental exposure to liquid Daphnia magna activity was inhibited (including death) concentration.
3.2
Immobilisation immobilization
Gently shake the test vessel, if within 15s of Daphnia magna can not swim, that its motion was inhibited, even if its tentacles still alive
Move, it should also be counted as individual activities suppressed.
Principle 4
This standard is used as experimental biological Daphnia magna, Daphnia magna into the range of concentrations of the test solution, the counting 24h and 48h Daphnia magna live
Move was inhibited (including death) the number of computing 24h and 48h EC50 (24hEC50 and 48hEC50), judged the experiment
The degree of toxicity of the solution were divided into two phases. the pre-test and formal experiments.
5 Reagents and materials
5.1 General Requirements
This standard reagents used were of analytical grade compliance with national standards of chemical reagents, laboratory water requirements with reference to GB/T 6682.
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