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GB/T 14926.22: Historical versions
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| GB/T 14926.22-2026 | English | 259 |
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Laboratory animal - Method for examination of Mouse hepatitis virus (MHV)
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| GB/T 14926.22-2001 | English | 139 |
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Laboratory animal -- Method for examination of mouse hepatitis virus (MHV)
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| GB/T 14926.22-1994 | English | 199 |
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Method for examination of mouse hepatitis virus in laboratory animal
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Basic data
| Standard ID | GB/T 14926.22-2026 (GB/T14926.22-2026) |
| Description (Translated English) | Laboratory animal - Method for examination of Mouse hepatitis virus (MHV) |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B44 |
| Classification of International Standard | 65.020.30 |
| Date of Issue | 2026-01-28 |
| Date of Implementation | 2026-05-01 |
| Older Standard (superseded by this standard) | GB/T 14926.22-2001 |
GB/T 14926.22-2026: Laboratory animal - Method for examination of Mouse hepatitis virus (MHV)
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.30
CCSB44
National Standards of the People's Republic of China
Replaces GB/T 14926.22-2001
Methods for detecting hepatitis virus in laboratory animals (mice)
Published on 2026-01-28
Implemented on May 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 22 of GB/T 14926.GB/T 14926 has already published the following parts.
---Detection method for Salmonella in laboratory animals (GB/T 14926.1);
---Detection method for Yersinia in laboratory animals (GB/T 14926.3);
---Detection Method for Pathogenic Fungi in Laboratory Animal Skin (GB/T 14926.4);
---Detection method for Pasteurella multocida in laboratory animals (GB/T 14926.5);
---Detection method for Bordetella bronchiseptica in laboratory animals (GB/T 14926.6);
---Methods for the detection of mycoplasma in laboratory animals (GB/T 14926.8);
---Detection method for Corynebacterium tumefaciens in laboratory animals (GB/T 14926.9);
---Detection method for Tyzer pathogens in laboratory animals (GB/T 14926.10);
---Detection method for Escherichia coli O115a,c.K(B) in laboratory animals (GB/T 14926.11);
---Detection method for Pasteurella pneumophila in laboratory animals (GB/T 14926.12);
---Detection method for Klebsiella pneumoniae in laboratory animals (GB/T 14926.13);
---Detection method for Staphylococcus aureus in laboratory animals (GB/T 14926.14);
---Detection method for Streptococcus pneumoniae in laboratory animals (GB/T 14926.15);
---Detection method for group A beta-hemolytic streptococci in laboratory animals (GB/T 14926.16);
---Detection method for Pseudomonas aeruginosa in laboratory animals (GB/T 14926.17);
---Detection method for choroid plexus meningitis virus in laboratory animal lymphocytes (GB/T 14926.18);
---Detection method for hantavirus in laboratory animals (GB/T 14926.19);
---Detection method for mousepox virus in laboratory animals (GB/T 14926.20);
---Detection method for rabbit hemorrhagic disease virus in laboratory animals (GB/T 14926.21);
---Detection method for hepatitis virus in laboratory animals (GB/T 14926.22);
---Detection method for Sendai virus in laboratory animals (GB/T 14926.23);
---Detection method for pneumonia virus in laboratory mice (GB/T 14926.24);
---Detection method for reovirus type III in laboratory animals (GB/T 14926.25);
---Detection method for mouse encephalomyelitis virus in laboratory animals (GB/T 14926.26);
---Detection method for adenovirus in laboratory animals (GB/T 14926.27);
---Detection method for mouse parvovirus in laboratory animals (GB/T 14926.28);
---Detection method for polyomavirus in laboratory animals (GB/T 14926.29);
---Detection method for rotavirus in laboratory rabbits (GB/T 14926.30);
---Detection method for rat parvovirus (KRV and H-1 strains) in laboratory animals (GB/T 14926.31);
---Detection method for coronavirus/dacryoadenitis virus in laboratory animals (GB/T 14926.32);
---Detection methods for germ-free living environment and fecal specimens of laboratory animals (GB/T 14926.41);
---Collection of laboratory animal bacteriological specimens (GB/T 14926.42);
---Staining methods, culture media and reagents for bacteriological testing of laboratory animals (GB/T 14926.43);
---Detection method for Streptococcus beadis in laboratory animals (GB/T 14926.44);
---Detection method for Brucella in laboratory animals (GB/T 14926.45);
---Detection method for Leptospira in laboratory animals (GB/T 14926.46);
---Detection method for Shigella in laboratory animals (GB/T 14926.47);
---Detection method for Mycobacterium tuberculosis in laboratory animals (GB/T 14926.48);
---Detection method for Campylobacter jejuni in laboratory animals (GB/T 14926.49);
---Enzyme-linked immunosorbent assay (ELISA) for laboratory animals (GB/T 14926.50);
---Experimental animal immunoenzyme test (GB/T 14926.51);
---Immunofluorescence assay for laboratory animals (GB/T 14926.52);
---Laboratory animal hemagglutination test (GB/T 14926.53);
---Laboratory animal hemagglutination inhibition test (GB/T 14926.54);
---Experimental animal immunoenzyme histochemistry method (GB/T 14926.55);
---Detection method for rabies virus in laboratory animals (GB/T 14926.56);
---Detection method for canine parvovirus in laboratory animals (GB/T 14926.57);
---Detection Method for Canine Infectious Hepatitis Virus in Laboratory Animals (GB/T 14926.58);
---Detection method for canine distemper virus in laboratory animals (GB/T 14926.59);
---Detection method for herpesvirus type 1 (B virus) in laboratory macaques (GB/T 14926.60);
---Detection method for reverse type D virus in laboratory animals (GB/T 14926.61);
---Detection method for simian immunodeficiency virus in laboratory animals (GB/T 14926.62);
---Detection method for type I tropism virus in experimental monkey T lymphocytes (GB/T 14926.63);
---Detection method for monkeypox virus in laboratory animals (GB/T 14926.64);
---Detection method for Pneumocystis jirovecii in laboratory animals (GB/T 14926.65).
This document replaces GB/T 14926.22-2001 "Detection Method for Hepatitis Virus in Laboratory Animals (Mice)" and is consistent with GB/T 14926.22-
Compared to.2001, aside from structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2001 edition);
b) Added nucleic acid testing methods, including the principle of nucleic acid testing, the main reagents and equipment used in nucleic acid testing, nucleic acid testing methods, and nucleic acid detection...
Interpretation of test results (see 4.2, 5.1.2, 5.2.2, 6.2.4, 7.2);
c) The term "clean-grade mice" has been removed (see sections 4.1.3 and 4.1.4 in the.2001 edition);
d) "Sterile mice" were added (see 5.1.1.4, 5.1.1.5);
e) Added normative reference documents (see 6.1.1);
f) Normative references have been amended (see 6.2, 6.3, 6.4, Chapter 5 of the.2001 edition);
g) Added specific operating procedures for mouse hepatitis virus nucleic acid detection (see Appendix A).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Science and Technology of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Standardization of Laboratory Animals (SAC/TC281).
This document was drafted by. Experimental Animal Center of Xi'an Jiaotong University, Suzhou Xishan Biotechnology Co., Ltd., and Biophysics Institute of the Chinese Academy of Sciences.
Research institute, Chongqing Medical University.
The main drafters of this document are. Liu Enqi, Cao Xuhong, Xia Congcong, Wang Rong, Zhao Sihai, Guo Lianxiang, Zhu Xiaoxiao, Zhang Qian, and Yang Genling.
The release history of this document and the document it replaces is as follows.
---First published in.1994 as GB/T 14926.22-1994, and revised for the first time in.2001;
---This is the second revision.
Introduction
In the fields of life science research, biopharmaceutical industry, and product quality control, how to effectively control the quality of laboratory animals affected by pathogenic microorganisms is a key issue.
Fluctuations and interference from animal experimental backgrounds have become a widely accepted consensus and a focus of attention. This issue affects the reliability and reproducibility of research data.
The effectiveness of biological products constitutes a lasting impact. Uncertainty regarding the microbiological status of laboratory animals directly affects animal health and...
The biosafety of experimental systems and personnel is crucial, and it can impact scientific research conclusions, drug safety evaluations, and the construction of human disease models.
This leads to a significant deviation.
Relevant quality monitoring and control programs must be based on solid scientific knowledge of microbiology, immunology, and molecular biology, and employ sensitive and specific methods.
Standardized detection methods are needed to address the various threats posed by zoonotic diseases, highly contagious animal diseases, and opportunistic pathogens. Laboratory animal quality...
The core of the monitoring and management program relies on the accurate quantification, standardized monitoring, and standardized reporting of specific pathogens.
GB/T 14926 refines the grade requirements into specific data indicators, specifying the standards for laboratory animals and their related products at different pathogen levels.
The purpose of the detection methods and reporting requirements for microorganisms in products is to specify the detection principles for different types of pathogens such as bacteria and viruses.
Reagents and materials, testing procedures, result interpretation, test reports, etc. GB/T 14926 is divided into the following parts.
---Methods for detecting Salmonella in laboratory animals;
---Detection method for Yersinia in laboratory animals;
---Detection methods for pathogenic fungi on the skin of laboratory animals;
---Detection method for Pasteurella multocida in laboratory animals;
---Detection method for Bordetella bronchiolitis in laboratory animals;
---Methods for detecting mycoplasma in laboratory animals;
---Detection method for Corynebacterium in laboratory animals (mouse);
---Methods for detecting Taizer pathogens in laboratory animals;
---Detection method for Escherichia coli O115a,c.K(B) in laboratory animals;
---Detection method for Pasteurella pneumophila in laboratory animals;
---Detection method for Klebsiella pneumoniae in laboratory animals;
---Methods for detecting Staphylococcus aureus in laboratory animals;
---Detection method for Streptococcus pneumoniae in laboratory animals;
---Detection method for group A beta-hemolytic streptococci in laboratory animals;
---Detection method for Pseudomonas aeruginosa in laboratory animals;
---Method for detecting choroid plexus meningitis virus in laboratory animal lymphocytes;
---Methods for detecting hantavirus in laboratory animals;
---Detection method for mousepox virus in laboratory animals;
---Detection method for rabbit hemorrhagic disease virus in laboratory animals;
---Methods for detecting hepatitis virus in laboratory animals (mice);
---Methods for detecting Sendai virus in laboratory animals;
---Methods for detecting pneumonia virus in laboratory mice;
---Detection method for type III reovirus in laboratory animals;
---Method for detecting encephalomyelitis virus in experimental animals (mice);
---Methods for detecting adenovirus in laboratory animals (mice);
---Methods for detecting parvovirus in laboratory animals (mice);
---Detection method for polyomavirus in laboratory animals;
---Detection method for rabbit rotavirus in laboratory animals;
---Detection methods for rat parvovirus (KRV and H-1 strains) in laboratory animals;
---Detection method for coronavirus/dacryoadenitis virus in experimental animals (rat);
---Methods for detecting germ-free animal living environments and fecal specimens in laboratory animals;
---Collection of laboratory animal bacteriological specimens;
---Staining methods, culture media, and reagents for bacteriological testing of laboratory animals;
---Detection method for Streptococcus beadis in laboratory animals;
---Methods for detecting Brucella in laboratory animals;
---Methods for detecting Leptospira in laboratory animals;
---Methods for detecting Shigella in laboratory animals;
---Methods for detecting Mycobacterium tuberculosis in laboratory animals;
---Detection method for Campylobacter jejuni in laboratory animals;
---Experimental animal enzyme-linked immunosorbent assay;
---Experimental animal immunoenzyme test;
---Immunofluorescence assay in experimental animals;
---Hemagglutination test in laboratory animals;
---Experimental animal hemagglutination inhibition test;
---Experimental animal immunoenzyme histochemistry;
---Methods for detecting rabies virus in laboratory animals;
---Detection method for canine parvovirus in laboratory animals;
---Detection method for infectious canine hepatitis virus in laboratory animals;
---Detection method for canine distemper virus in laboratory animals;
---Detection method for herpesvirus type 1 (B virus) in laboratory macaques;
---A method for detecting reverse D-virus in experimental monkeys;
---Methods for detecting immunodeficiency virus in laboratory animals (monkeys);
---Detection method for type I tropism virus in monkey T lymphocytes (experimental animals);
---Methods for detecting monkeypox virus in laboratory animals;
---Detection method for Pneumocystis jirovecii in laboratory animals.
Methods for detecting hepatitis virus in laboratory animals (mice)
1 Scope
This document applies to the detection of MHV in mice or experimental inoculum, and in laboratory animal environmental samples.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 14926.42 Collection of Specimens for Bacteriological Examination of Laboratory Animals
GB/T 14926.50 Enzyme-linked immunosorbent assay (ELISA) for laboratory animals
GB/T 14926.51 Laboratory animal immunoenzyme test
GB/T 14926.52 Immunofluorescence assay for laboratory animals
3 Terms and Definitions
This document does not contain any terms or definitions that need to be defined.
4.Principles
4.1 Antibody Detection Principle
Based on immunological principles, MHV antigen was used to detect MHV antibodies in mouse serum.
4.2 Principle of Nucleic Acid Detection
Common MHV strains in laboratory animals have unique genomic nucleic acid sequences that can be amplified using nucleic acid amplification techniques such as polymerase chain reaction.
The technique is used to identify.
5.Reagents and Equipment
5.1 Reagents
5.1.1 Antibody detection reagents
MHV virus (including four strains. MHV1, MHV3, MHV-A59, and MHV-JHM) was used to infect DBT or L929 cells, followed by inoculation.
Harvest 2-4 days later when the lesions have reached a certain level. After three freeze-thaw cycles or sonication, remove cell debris by low-speed centrifugation, and collect the supernatant.
The solution is then concentrated by ultracentrifugation to produce ELISA antigen.
...
