GB 5009.247-2025 English PDF

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GB 5009.247-2025: National food safety standard - Determination of Neotame in foods
Status: Valid

GB 5009.247: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 5009.247-2025	259	Add to Cart	3 days	National food safety standard - Determination of Neotame in foods	Valid
GB 5009.247-2016	80	Add to Cart	Auto, < 3 mins	Method for the determination of Neotame in foods -- High-performance liquid chromatography	Valid

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Basic data

Standard ID: GB 5009.247-2025 (GB5009.247-2025)
Description (Translated English): National food safety standard - Determination of Neotame in foods
Sector / Industry: National Standard
Classification of Chinese Standard: X09
Word Count Estimation: 13,191
Issuing agency(ies): National Health Commission of the People's Republic of China, State Administration for Market Regulation

GB 5009.247-2016: Method for the determination of Neotame in foods -- High-performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Determination of neotame in foods) National Standards of People's Republic of China National Food Safety Standard DETERMINATION neotame Issued on. 2016-08-31 2017-03-01 implementation People's Republic of China National Health and Family Planning Commission released

Foreword

This standard replaces GB/T 23378-2009 "Determination of foods neotame by HPLC." This standard compared with GB/T 23378-2009, the main changes are as follows. --- Standard name was changed to "national food safety standards in food Neotame determination." --- Scope Change drinks, sweets, cakes, roasted seeds and nuts, pickles, candy, jams, jellies, sauces complex measured neotame. National Food Safety Standard DETERMINATION neotame

1 Scope

This standard specifies a high performance liquid chromatography in food neotame content. This standard applies to the determination of drinks, sweets, cakes, roasted seeds and nuts, pickles, candy, jams, jellies, seasonings in food neotame. Principle 2 The sample was mixed extraction liquid extraction, solid phase extraction (SPE), high performance liquid chromatograph retention time qualitative, external standard peak area Statutory amount.

3 Reagents and materials

Unless otherwise indicated, the methods used were of analytical grade reagents and water as a water GB/T 6682 regulations. 3.1 Reagents 3.1.1 acetonitrile (CH3CN). chromatography. 3.1.2 octane sulfonate (C8H17NaO3S). chromatography. 3.1.3 phosphoric acid (H3PO4). 3.1.4 formic acid (CH3COOH). chromatography. 3.1.5 Methanol (CH3OH). chromatography. 3.1.6 triethylamine (C6H15N). chromatography. 3.2 reagent preparation 3.2.1 Mixed extracts. Pipette 0.8mL and 2.5mL of triethylamine formate, add water to 1000mL, pH of about 4.5. 3.2.2 Ion pair reagent buffer. Weigh 2.00g octane sulfonate, was dissolved with 500mL of water, phosphoric acid was added 1.0mL, add water to 1000mL. 3.3 Standard Neotame (C20H30N2O5, CAS number. 165450-17-9), purity ≥99.0%. 3.4 Standard Solution 3.4.1 Standard stock solution. Accurately weigh 0.1000g neotame standards, plus mixed extract dissolved volume to 100mL, the solution containing neotame An amount of 1.00mg/mL. 3.4.2 Working standard solution. Pipette appropriate amount of neotame standard stock solution, mixed with extracts formulated to concentrations of 0.2μg/mL, 1.0μg/mL, 5.0μg/mL, 10.0μg/mL, 50.0μg/mL, 100.0μg/mL series of standard working solution. 3.5 C18 solid phase extraction column. 6mL, 500mg, or rather who, prior to use successively with methanol 5mL, 10mL water activation. 3.6 0.45μm membrane, organic.

4 instruments and equipment

4.1 liquid chromatograph equipped with an ultraviolet detector or diode array detector. 4.2 ultrasonic cleaning. 4.3 Analysis of balance. a sense of volume and 0.0001g 0.01g. 4.4 Organization mashed machines. 4.5 Vortex. 4.6 nitrogen blowing instrument. 4.7 Solid phase extraction device. 4.8 centrifuge. speed ≥4000r/min. Step 5 Analysis 5.1 Sample Preparation 5.1.1 solid samples Weigh 10g (accurate to 0.01g) in 50mL sample pulverized uniformly stoppered plastic centrifuge tube, add 30mL mixed extract, spin Vortex oscillation 10min, ultrasound 30min, with a mixed extract to the mark, if the solution is cloudy, not less than 4000r/min centrifugal 10min after filtration and set aside. 5.1.2 Liquid Sample Accurately weighed sample in 50mL 10.0mL stoppered plastic centrifuge tube, add 30mL mixed extract Shakers. Ultrasonic After 15min, the mixed extract to the mark, if the solution is cloudy, not less than 4000r/min centrifugal 10min, filtered and set aside. Note. Samples containing gas such as carbonated beverages, soft drinks and other first lukewarm, stirring to remove the carbon dioxide in the sample or ultrasonic degassing, and then accurately weighed sample. 5.2 Sample purification Absorb 10.0mL filtrate flow rate of 1mL/min ~ 2mL/min through a solid phase extraction column until the filtrate flows out completely by The extract was mixed with a flow rate of 5mL 1mL/min ~ 2mL/min leaching extraction column, discard all effluent with 5mL methanol 1mL/min flow rate were eluted at 40 ℃ water bath with nitrogen blowing instrument concentrated extract volume to 2.0mL with a mixed, through 0.45μm After membrane filtration as a test solution for liquid chromatography analysis. 5.3 Instrument Reference conditions 5.3.1 Column. C18 column, 5μm, 250mm × 4.6mm (diameter) or equivalent person. 5.3.2 Column temperature. 30 ℃. 5.3.3 Mobile phase. A phase. acetonitrile; B phase. ion pair reagent buffer. Gradient program shown in Table 1. 5.3.4 flow rate. 1.0mL/min. 5.3.5 Detection wavelength. 218nm. 5.3.6 Injection volume. 50μL. Table 1 gradient elution program Time/min Mobile phase A /% mobile phase B /% 0.00 25.0 75.0 10.00 65.0 35.0 20.00 100.0 0.0 24.00 100.0 0.0 24.10 25.0 75.0 30.00 25.0 75.0 5.4 standard curve The standard series working solution were injected into the liquid chromatograph to measure the peak area corresponding to the concentration of the working standard solution as abscissa and the Peak area of the vertical axis, the standard curve (Neotame standard chromatogram shown in Figure A.1). 5.5 Determination of the sample solution The sample solution into the liquid chromatograph retention time qualitative, and record the peak area to obtain test solution based on the standard curve Zealand Sweet concentration response in the sample solution neotame content should be within the linear range of the instrument.

6 expression analysis

Neotame content of the sample according to the formula (1). X = c × V × V1 × 1000 m × V2 × 1000 (1) Where. X --- sample neotame content in milligrams per kilogram (mg/kg) or milligrams per liter (mg/L); C --- concentration of the sample solution neotame, in micrograms per milliliter (μg/mL); The V --- eluate was concentrated sample volume to volume, in milliliters (mL of); Vl --- volume of the sample extract volume in milliliters (mL of); m --- sample weight in grams (g); --- Draw volume V2 of the filtrate in milliliters (mL). The results to two significant figures.

7 precision

Two independent determination results under the absolute difference in repeatability condition must not exceed 10% of the arithmetic mean.

8 Other

The limit of quantification of the standard methods of 0.2mg/kg.

Appendix A

Chromatogram Neotame standard chromatogram shown in Figure A.1. Figure A.1 Neotame standard liquid chromatogram ......

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