GB/T 8313-2018 PDF English
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Determination of total polyphenols and catechins content in tea
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GB/T 8313-2008 | English | 319 |
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Tea -- Determination of total polyphenols and catechins content
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GB/T 8313-2002 | English | 199 |
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Tea -- Determination of tea polyphenols content
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Tea--Determination of tea polyphenols content
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GB/T 8313-2018: PDF in English (GBT 8313-2018) GB/T 8313-2018
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.140.10
X 55
Replacing GB/T 8313-2008
Determination of total polyphenols and catechins
content in tea
(ISO 14502-1:2005, Determination of substances characteristic of green and
black tea - Part 1: Content of total polyphenols in tea - Colorimetric method
using Folin-Ciocalteu agent; ISO 14502-2:2005, Determination of substances
characteristic of green and black tea - Part 2: Content of catechins in green
tea - Method using high-performance liquid chromatography, NEQ)
ISSUED ON: JULY 13, 2018
IMPLEMENTED ON: NOVEMBER 01, 2018
Issued by: State Administration for Market Regulation;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Detection of catechins in tea - HPLC method ... 4
4 Detection of polyphenols in tea ... 9
Appendix A (Informative) Liquid chromatogram of standard samples of catechin
and caffeine ... 12
Determination of total polyphenols and catechins
content in tea
1 Scope
This standard specifies a method for the determination of catechins in tea, by
high-performance liquid chromatography (HPLC), AND the method for the
determination of polyphenols in tea, by spectrophotometry.
This standard applies to the determination of catechins and polyphenols, in tea
and tea products.
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) is applicable to this standard.
GB/T 6682 Water for analytical laboratory use - Specification and test
methods
GB/T 8302 Tea - Sampling (GB/T 8302-2013, ISO 1839:1980, NEQ)
GB/T 8303 Tea - Preparation of ground sample and determination of dry
matter content (GB/T 8303-2013, ISO 1572:1980, MOD)
3 Detection of catechins in tea - HPLC method
3.1 Principle
The catechins in the ground tea sample are extracted by 70% methanol
aqueous solution, on a 70 °C water bath. The catechins are determined by C18
column, detection wavelength 278 nm, gradient elution, HPLC analysis; it is
directly quantified by the external standard method of catechins standard
materials, OR otherwise quantified by the relative correction factor (RRFStd) of
catechins and caffeine in the results of the ISO international ring test.
3.2 Instruments
3.2.1 Analytical balance: Accuracy 0.0001 g.
(EGCG) 2.00 mg/mL, epicatechin gallate (ECG) 2.00 mg/mL.
3.3.11 Standard working solution: Prepared by the stable solution (3.3.8).
Standard working solution's concentration: Gallic acid 5 μg/mL ~ 25 μg/mL,
caffeine 50 μg/mL ~ 150 μg/mL, +C 50 μg/mL ~ 150 μg/mL, EC 50 μg/mL ~ 150
μg/mL, EGC 100 μg/mL ~ 300 μg/mL, EGCG 100 μg/mL ~ 400 μg/mL, ECG 50
μg/mL ~ 200 μg/mL.
3.3.12 0.45 μm organic phase filter membrane.
3.4 Operation method
3.4.1 Sampling
It is carried out according to the provisions of GB/T 8302.
3.4.2 Sample preparation
It is carried out according to the provisions of GB/T 8303.
3.4.3 Determination procedure
3.4.3.1 Determination of dry matter content
It is carried out according to the provisions of GB/T 8303.
3.4.3.2 Preparation of test solution
3.4.3.2.1 Mother liquor: Weigh 0.2 g (accurate to 0.0001 g) of uniformly ground
sample (3.4.2), into a 10 mL centrifuge tube. Add 5 mL of 70% methanol
aqueous solution (3.3.5), which was pre-heated at 70 °C. Use a glass rod to
fully stir, to evenly moisten it. Immediately transfer it into a 70 °C water bath
(3.2.2). Extract for 10 minutes (stir it once every 5 minutes). Cool to room
temperature after extraction. Transfer it into a centrifuge at 3500 r/min.
Centrifuge for 10 min. Transfer the supernatant to a 10 mL volumetric flask. Use
5 mL of 70% methanol aqueous solution, to extract the residue once again.
Repeat the above operation. Combine the extracts. Make the volume reach to
10 mL. Shake well. Use a 0.45 μm membrane (3.3.12) to filter it. Prepare for
use (the extract can be stored at 4 °C for up to 24 h).
3.4.3.2.2 Test solution: Use a pipette to transfer 2 mL of mother liquor (3.4.3.2.1),
into a 10 mL volumetric flask. Use the stable solution (3.3.8) to make its volume
reach to the mark. Shake well. Pass through a 0.45 μm membrane (3.3.12), to
be tested.
3.4.3.3 Chromatographic conditions
The relative error of the two measured values of the total amount of catechins,
in the same sample, shall be ≤ 10%. If the relative error of the measured value
is within this range, take the arithmetic mean of the two measured values as
the result; keep two decimal places.
4 Detection of polyphenols in tea
4.1 Principle
The polyphenols in the ground tea samples are extracted by 70% methanol
aqueous solution, in a 70 °C water bath. The Folin phenol reagent oxidizes the
-OH groups in the polyphenols and appears blue. The maximum absorption
wavelength λ is 765 nm. Gallic acid is used as a calibration standard, to quantify
the polyphenols.
4.2 Instruments
4.2.1 Analytical balance: Accuracy of 0.001 g.
4.2.2 Water bath: 70 °C ± 1 °C.
4.2.3 Centrifuge: Speed 3500 r/min.
4.2.4 Spectrophotometer.
4.3 Reagents
4.3.1 Methanol.
4.3.2 Sodium carbonate (Na2CO3).
4.3.3 70% methanol aqueous solution.
4.3.4 Folin-Ciocalteu: 1 mol/L.
4.3.5 10% Folin-Ciocalteu reagent (equipped before use): Transfer 20 mL of
Folin-Ciocalteu reagent (4.3.4) into a 200 mL volumetric flask. Use water to
make its volume reach to the mark. Shake well.
4.3.6 The 7.5% sodium carbonate (Na2CO3) solution: Weigh 37.50 g ± 0.01 g
of sodium carbonate (Na2CO3) (4.3.2). Add an appropriate amount of water to
dissolve it. Transfer it into a 500 mL volumetric flask. Make its volume reach to
the mark. Shake well (it can be stored for 1 month, at room temperature).
4.3.7 Gallic acid standard stock solution (1000 μg/mL): Weigh 0.110 g ± 0.001
g of gallic acid (GA, relative molecular weight 188.14). Dissolve it in a 100 mL
volumetric flask. Make its volume reach to the mark. Shake well (prepared
...... Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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