GB/T 37873-2019 PDF English
US$199.00 · In stock · Download in 9 secondsGB/T 37873-2019: General assessment of quality evaluation for synthesized genes Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 37873-2019 | English | 199 |
Add to Cart
|
3 days
|
General assessment of quality evaluation for synthesized genes
| Valid |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB/T 37873-2019
GB/T 37873-2019: General assessment of quality evaluation for synthesized genes---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT37873-2019
(General rules for the evaluation of synthetic gene quality)
ICS 03.120
G30
National Standards of People's Republic of China
General rules for the evaluation of synthetic gene quality
Generalassessmentofqualityevaluationforsynthesizedgenes
Published on.2019-08-30
2019-08-30 implementation
State market supervision and administration
China National Standardization Administration issued
Content
Foreword III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Quality requirements 1
4.1 General 1
4.2 Purity 1
4.3 Total 1
4.4 Integrity 2
4.5 Sequence Consistency 2
5 Evaluation method 2
5.1 Samples and Reagents 2
5.2 Purity detection 2
5.3 Total amount detection 2
5.4 Integrity Detection 3
5.5 Sequence Consistency Detection 3
Appendix A (informative) Synthetic gene electrophoresis Figure 4
Appendix B (informative) Agarose gel electrophoresis detection method 5
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Standards Research Center.
This standard was drafted. Shenzhen Huada Life Science Research Institute (formerly Shenzhen Huada Gene Research Institute), China Institute of Metrology, Shenzhen
Huada Gene Technology Co., Ltd., Qingdao Huada Gene Research Institute.
The main drafters of this standard. Wang Yun, Wang Jing, Shen Yi, Fu Boqiang, Zhao Hongcui, Gong Jianhui, Chen Tai, Liu Xin, Du Jiating, Xie Qiang, Niu Chunyan.
General rules for the evaluation of synthetic gene quality
1 Scope
This standard specifies the terms and definitions of synthetic genes, quality requirements, and evaluation methods.
This standard applies to the quality evaluation of synthetic genes (100bp ~ 20kb) based on biochemical synthesis.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 34796-2017 Determination of the concentration and purity of nucleic acids in aqueous solutions - UV spectrophotometry
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Synthetic gene
A double-stranded DNA artificially synthesized according to a defined nucleotide sequence by a biochemical method.
3.2
DNA sequencing DNAsequencing
The determination of the nucleotide arrangement order of the DNA molecules, that is, the order of arrangement of A, T, G, and C constituting the DNA molecules. often
The methods used are the Sanger-Cousson method and the Marksam-Gilbert method.
[JJF1265-2010, definition 5.17]
3.3
Sequence alignment sequence alignment
The process of comparing the similarities between two or more nucleotide or amino acid sequences.
[GB/T 29859-2013, definition 2.2.1]
4 Quality requirements
4.1 General
The DNA sequence of the synthetic gene, in accordance with the requirements of the International Gene Synthesis Alliance (IGSC) biosafety requirements, including its quality requirements, including
DNA purity, total, integrity and sequence identity.
4.2 Purity
Under the condition of pH 7.0~8.5, the ratio of OD260/OD280 is between 1.8 and 2.0, and OD260/OD230≥2.0, in line with
Requirements of 8.3.2 of GB/T 34796-2017.
4.3 Total
The total quality of synthetic DNA is not lower than the customer's needs. Generally not less than 1 μg.
GB/T 37873-2019
(General rules for the evaluation of synthetic gene quality)
ICS 03.120
G30
National Standards of People's Republic of China
General rules for the evaluation of synthetic gene quality
Generalassessmentofqualityevaluationforsynthesizedgenes
Published on.2019-08-30
2019-08-30 implementation
State market supervision and administration
China National Standardization Administration issued
Content
Foreword III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Quality requirements 1
4.1 General 1
4.2 Purity 1
4.3 Total 1
4.4 Integrity 2
4.5 Sequence Consistency 2
5 Evaluation method 2
5.1 Samples and Reagents 2
5.2 Purity detection 2
5.3 Total amount detection 2
5.4 Integrity Detection 3
5.5 Sequence Consistency Detection 3
Appendix A (informative) Synthetic gene electrophoresis Figure 4
Appendix B (informative) Agarose gel electrophoresis detection method 5
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Standards Research Center.
This standard was drafted. Shenzhen Huada Life Science Research Institute (formerly Shenzhen Huada Gene Research Institute), China Institute of Metrology, Shenzhen
Huada Gene Technology Co., Ltd., Qingdao Huada Gene Research Institute.
The main drafters of this standard. Wang Yun, Wang Jing, Shen Yi, Fu Boqiang, Zhao Hongcui, Gong Jianhui, Chen Tai, Liu Xin, Du Jiating, Xie Qiang, Niu Chunyan.
General rules for the evaluation of synthetic gene quality
1 Scope
This standard specifies the terms and definitions of synthetic genes, quality requirements, and evaluation methods.
This standard applies to the quality evaluation of synthetic genes (100bp ~ 20kb) based on biochemical synthesis.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 34796-2017 Determination of the concentration and purity of nucleic acids in aqueous solutions - UV spectrophotometry
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Synthetic gene
A double-stranded DNA artificially synthesized according to a defined nucleotide sequence by a biochemical method.
3.2
DNA sequencing DNAsequencing
The determination of the nucleotide arrangement order of the DNA molecules, that is, the order of arrangement of A, T, G, and C constituting the DNA molecules. often
The methods used are the Sanger-Cousson method and the Marksam-Gilbert method.
[JJF1265-2010, definition 5.17]
3.3
Sequence alignment sequence alignment
The process of comparing the similarities between two or more nucleotide or amino acid sequences.
[GB/T 29859-2013, definition 2.2.1]
4 Quality requirements
4.1 General
The DNA sequence of the synthetic gene, in accordance with the requirements of the International Gene Synthesis Alliance (IGSC) biosafety requirements, including its quality requirements, including
DNA purity, total, integrity and sequence identity.
4.2 Purity
Under the condition of pH 7.0~8.5, the ratio of OD260/OD280 is between 1.8 and 2.0, and OD260/OD230≥2.0, in line with
Requirements of 8.3.2 of GB/T 34796-2017.
4.3 Total
The total quality of synthetic DNA is not lower than the customer's needs. Generally not less than 1 μg.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB/T 37873-2019 be delivered?Answer: The full copy PDF of English version of GB/T 37873-2019 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB/T 37873-2019_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 37873-2019_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB/T 37873-2019 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. How to buy and download a true PDF of English version of GB/T 37873-2019?A step-by-step guide to download PDF of GB/T 37873-2019_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB/T 37873-2019".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|