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GB/T 35946-2018 PDF English


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GB/T 35946-2018: PDF in English (GBT 35946-2018)

GB/T 35946-2018 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 71.100.70 Y 42 Determination of thimerosal in eye cosmetics - High performance liquid chromatography ISSUED ON. FEBRUARY 6, 2018 IMPLEMENTED ON. SEPTEMBER 1, 2018 Issued by. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of the People's Republic of China. Table of Contents Foreword ... 3 1 Scope ... 4 2 Normative references ... 4 3 Test method ... 4 4 Result calculation ... 8 5 Recovery and accuracy ... 8 6 Admissible error ... 8 Annex A (Informative) Chinese name, English name, INCI name, CAS number, molecular formula, relative molecular mass and constitutional formula of standard substance ... 9 Annex B (Informative) Chromatogram of thimerosal standard sample ... 10 Determination of thimerosal in eye cosmetics - High performance liquid chromatography 1 Scope This Standard specifies the test method, result calculation, recovery and accuracy, tolerances, etc. for the determination of thimerosal in eye cosmetics using the high- performance liquid chromatography. This Standard applies to the determination of thimerosal in eye cosmetics, including cleansing lotion, eye shadow, eye cream, mascara, liquid eyeliner, eyeliner cream and eyebrow pencil. The detection limits and quantitation limits of the Method of this Standard. the detection limit of cleansing lotion is 1 mg/kg and the detection limit of eye shadow, eye cream, liquid eyeliner, etc. is 2 mg/kg; the quantitation limit of cleansing lotion is 3 mg/kg and the quantitation limit of eye shadow, eye cream, liquid eyeliner, etc. is 6 mg/kg. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition dated applies to this document. For undated references, the latest edition of the referenced documents (including all amendments) applies to this document. GB/T 6682, Water for analytical laboratory use – Specification and test methods 3 Test method 3.1 Principle After extracting and centrifuging sample using methyl alcohol, use solid-phase extraction cartridge to purify liquid supernatant; use the high-performance liquid chromatography for determination and confirmation; use the external standard method for quantitation. 3.2 Reagents and materials 3.2.14 Intermediate concentration thimerosal standard stock solution. 50 mg/L. Accurately measure 0.5 mL of thimerosal standard stock solution (3.2.13); pour into a 10 mL volumetric flask; add formic acid methanol solution 10% (3.2.6) before adding dropwise to scale; shake up; then obtain intermediate concentration thimerosal standard stock solution of mass concentration 50 mg/L, which shall be prepared immediately before use. 3.2.15 Thimerosal standard working solution. accurately measure a certain volume of intermediate concentration thimerosal standard stock solution (3.2.14), respectively; use formic acid methanol solution 10% (3.2.6) to prepare thimerosal standard working solutions of different concentrations including 0 mg/L, 0.5 mg/L, 1.0 mg/L, 2.0 mg/L, 5.0 mg/L, 10 mg/L, 20 mg/L and 50 mg/L, which shall be prepared immediately before use. 3.3 Apparatus 3.3.1 High performance liquid chromatograph (diode array detector). 3.3.2 Analytical balance. sensitivity 0.000 1 g. 3.3.3 Ultrasonic wave cleaner. 3.3.4 Solid phase extraction device. 3.3.5 Vortex mixer. 3.3.6 Centrifugal machine. 3.4 Sample preparation 3.4.1 Extraction 3.4.1.1 Eye cleansing lotion Shake up sample; accurately weigh 0.5 g of sample; add 3 mL of methanol; vortex disperse for 2 min; add methanol before adding dropwise to 5 mL to await purification. 3.4.1.2 Eye shadow, eye cream, mascara, eye gel, liquid eyeliner, eyeliner cream, eyebrow pencil, etc. Weigh about 0.5 g (accurate to 0.000 1 g) of sample; add 10.0 mL of methanol solution (3.2.8) 50%; fully vibrate for 1 min (ultrasound-assisted dissolution can be carried out for sticky sample); centrifuge for 5 min at 6 000 r/min; take 5 mL of liquid supernatant to await purification. 3.4.2 Purification ......
 
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.