GB/T 14698-2017 PDF English
US$140.00 · In stock · Download in 9 secondsGB/T 14698-2017: Identification method of feed material by microscopy Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB/T 14698: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 14698-2017 | English | 140 |
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Identification method of feed material by microscopy
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| GB/T 14698-2002 | English | 120 |
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Test method of feed microscopy
| Obsolete |
| GB/T 14698-1993 | English | 239 |
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Method for the test of feed microscopy
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GB/T 14698-2017: Identification method of feed material by microscopy---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT14698-2017
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.120
B 46
Replacing GB/T 14698-2002
Identification method of feed material by microscopy
Issued on. SEPTEMBER 07, 2017
Implemented on. APRIL 01, 2018
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Principles... 4
4 Instruments... 4
5 Reagents and solutions... 5
6 Reference sample... 6
7 Specimen preparation... 6
8 Inspection steps... 8
9 Identification methods and identification experiments... 9
10 Result expression... 11
Foreword
This standard was drafted in accordance with the rules given GB/T 1.1-2009.
This standard replaces GB/T 14698-2002 “Identification method of feed
material by microscopy”. As compared with GB/T 14698-2002, the main
technical changes of this standard are as follows.
- DELETE the relevant contents of compound feed from the text (SEE clause
1 of 2002 version);
- ADJUST the standard text structure;
- ADD the petroleum ether degreasing treatment method (SEE clause 7.2.3).
This standard was proposed by AND shall be under the jurisdiction of National
Feed Industry Standardization Technical Committee (SAC/TC 76).
The drafting organizations of this standard. National Feed Quality Supervision
and Inspection Center (Wuhan), New Hope Liuhe Co., Ltd., Guangzhou
Kangruide Biological Technology Co., Ltd., Hunan Zhenghong Technology
Development Co., Ltd.
The main drafters of this standard. Yang Haipeng, Guo Jiyuan, Liu Xianrong,
Yang Lin, Rong Jia, Zhu Zhengpeng, Hu Zhenjun, Wang Hu, Qian Ying, Jiang
Xiaoxia.
This standard replaces the standards previously issued as follows.
- GB/T 14698-1993, GB/T 14698-2002.
Identification method of feed material by microscopy
1 Scope
This standard specifies the identification method of feed material by microscopy.
This standard applies to the qualitative identification of feed material by
microscopy.
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) are applicable to this document.
GB/T 6682 Water for analytical laboratory use - Specification and test
methods
GB/T 14699.1 Feed - Sampling
GB/T 34269-2017 Identification chromatogram of feed material by
microscopy
Feed material directory (Announcement of Ministry of Agriculture of People's
Republic of China No. 1773)
3 Principles
The appearance morphology, organization structure, cell morphology, and
staining characteristics of the substance under inspection are observed under
the microscope, the results are compared with GB/T 34269-2017, to identify
and evaluate its type and quality.
4 Instruments
4.1 Stereo-microscope. magnification can be 7 times ~ 40 times.
4.2 Biological microscope. magnification can be 40 times ~ 500 times.
4.3 Magnifying glass.
4.8 Pointed tweezers, pointed probes and so on.
4.9 Electric oven, electric furnace, alcohol lamp and equipment commonly used
in lab.
5 Reagents and solutions
Unless otherwise indicated, the reagents used in this standard are of analytical
pure, AND the water is level III water as specified in GB/T 6682.
5.1 Carbon tetrachloride.
5.2 Petroleum ether. Boiling point 30 °C ~ 60 °C.
5.7 Iodine solution. WEIGH 0.75 g of potassium iodide and 0.1 g of iodine,
DISSOLVE it in 30 mL of water, STORE it into a brown bottle, PREPARE it
before use.
5.8 Ninhydrin solution 5 g/L. WEIGH 0.5 g of ninhydrin, DISSOLVE it in 100 mL
of water, STORE it into a brown bottle, PREPARE it before use.
5.9 Ammonium nitrate solution. DISSOLVE 10 g of ferric nitrate in 100 mL water.
5.10 Molybdate solution. DISSOLVE 20 g of molybdenum trioxide into the
mixture of 30 mL of ammonia and 50 mL of water, slowly POUR this solution
into the nitric acid solution (5.6), HEAT it slightly to dissolve it, COOL it down,
MIX it with 100 mL of ammonium nitrate solution (5.9).
5.13 Silver nitrate solution. WEIGH 10 g of silver nitrate, DISSOLVE it in 100
mL of water.
5.14 Phloroglucinol solution 20 g/L. WEIGH 2 g of phloroglucinol, DISSOLVE it
into 100 mL of 95% ethanol, STORE it into a brown bottle.
6 Reference sample
6.1 Feed material reference sample
FOLLOW the characterization as described in “Feed material catalog” to collect
and prepare reference samples.
6.2 Dopant reference sample
COLLECT the rice hull flour, wood chips, peanut hull flour, leather powder, etc.,
may be used to serve as a dopant or adulterant sample.
7 Specimen preparation
7.1 Sampling
TAKE sample in accordance with GB/T 14699.1, TAKE the representative feed
sample, USE the quartering method to reduce the sample to the amount as
required for inspection. The specimen is stored in a sealed glass bottle or a
sealed sample bag at room temperature.
7.2 Specimen pre-preparation
7.2.1 Screening
Based on the particle size of the specimen, SELECT the appropriate standard
sieve (4.4), PLACE the sieve of the maximum bore diameter above the sieve of
the minimum bore diameter, PLACE the sieve base at the bottom.
7.2.2 Particle or pellet specimen treatment
TAKE a few grains into a mortar (4.5), USE pestle to grind it to scatter it into
different compositions, BUT do not CRUSH the composition itself. After initial
grinding, MAKE it pass the sieve of bore diameter 0.42 mm.
7.2.5 Carbon tetrachloride flotation treatment
TAKE about 10 g of specimen into a 100 mL high beaker, ADD about 90 mL of
carbon tetrachloride (5.1), STIR it for about 10 s, LET it be standing for 2 min ~
5 min. After the upper and lower layers are clearly separated, USE spoon to
take out the materials floating at the upper layer or USE the decantation filtration
method to separate it out, after the surface flotation agent is volatilized, PLACE
it into the oven (4.9) at (70 ± 2) °C for 10 min ~ 20 min, TAKE it out to cool it to
room temperature, PLACE the sample into the culture dish (4.7) to prepare for
inspection.
8 Inspection steps
8.1 Sensory inspection
SPREAD 50 g ~ 100 g of specimen on a white paper, MAKE sensory inspection
for the specimen in sufficient natural light, the user directly inspects the
specimen visually and tactilely.
8.2 Stereomicroscope inspection
PLACE the culture dish on which specimen is paved under the microscope (4.1)
to observe it, USE the sufficient scattered natural light or reading lamp as the
light source (pay attention to make comparison observation of the reference
sample in the same light source), it shall maintain a 45° angle between the
incident light and the specimen plane when a reading lamp is used.
8.3 Biological microscopy
Specimen particles and specimen that cannot be accurately identified under a
stereomicroscope, respectively TAKE a small amount of specimen from above
the sieve and from the sieve base plate, PLACE it on the slide glass (4.7), ADD
two drops of a suspending agent I (5.11), USE the probe (4.8) to stir it to scatter
it, MAKE it uniformly soaked, USE a glass slide to cover it.
9 Identification methods and identification experiments
9.1 Identification of major inorganic components
PLACE the dried sediment (7.2.3, 7.2.4 and 7.2.5) on a sieve set composed of
sieve having hole-diameter 0.42 mm, 0.25 mm, 0.177 mm and base plate to
sieve it, respectively PLACE the sieved four parts into the culture dishes, USE
the stereomicroscope to inspect it, the bone and scale of animal and fish as
well as the shell of molluscs are generally easily identifiable. The salt is usually
cubic; the calcite in limestone is rhombohedral.
9.2 Identification test
9.2.1 Observation method
Identification test can be made with the naked eye or stereomicroscope
observation.
9.2.2 Silver nitrate test
TAKE 2 to 5 particles of unknown suspicious material and PLACE it on the drip
plate (4.6). ADD 2 drops of silver nitrate solution (5.13) to make observation.
- If white crystals are formed and slowly become larger, it is indicated that the
unknown particles are chloride;
- If yellow crystals and yellow flaky pieces are formed, it is indicated that the
unknown particles are phosphate;
- If slightly white acicular pieces are formed, it is indicated that the unknown
particles are sulfate;
- If the particles slowly darken, it is indicated that the unknown particles are
bone.
9.2.3 Hydrochloric acid test
TAKE 2 to 5 drops of unknown suspicious particles on the drip plate (4.6), ADD
2 drops of hydrochloric acid solution (5.4), or 3 ~ 5 pieces of suspicious material
in a 50 mL beaker, ADD 5 mL of hydrochloric acid solution (5.4), MAKE
observation.
9.2.4 Molybdate test
TAKE 2 to 5 particles of unknown suspicious material on the drip plate (4.6),
ADD 2 drops of molybdate solution (5.10), MAKE observation. If tiny yellow
crystals are formed near unknown suspect particles, the unknown suspect
particles are phosphate, phosphate rock, or bone (all phosphates have this
reaction, BUT dihydrogen phosphate and hydrogen phosphate dibasic can be
identified by silver nitrate).
9.2.7 Phloroglucinol test
TAKE 1 g ~ 2 g of sample into a 50 mL beaker, ADD 10 ~ 20 drops of
phloroglucinol solution (5.14) to infiltrate the sample, LET it be standing for 5
min, ADD 5 ~ 10 drops of hydrochloric acid solution (5.4), if it becomes dark red,
then the specimen contains lignin.
10 Result expression
The result expression shall include the specimen appearance, color, and the
substance observed under the microscope, AND give the judgment opinions on
whether the specimen under inspection is consistent with the name of the
sample delivered for inspection.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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