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GB/T 14698-2017 PDF in English

GB/T 14698-2017 (GB/T14698-2017, GBT 14698-2017, GBT14698-2017)
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GB/T 14698-2017: PDF in English (GBT 14698-2017)

GB/T 14698-2017
ICS 65.120
B 46
Replacing GB/T 14698-2002
Identification method of feed material by microscopy
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
Table of Contents
Foreword ... 3 
1 Scope ... 4 
2 Normative references ... 4 
3 Principles ... 4 
4 Instruments ... 4 
5 Reagents and solutions ... 5 
6 Reference sample ... 6 
7 Specimen preparation ... 6 
8 Inspection steps ... 8 
9 Identification methods and identification experiments ... 9 
10 Result expression ... 11 
This standard was drafted in accordance with the rules given GB/T 1.1-2009.
This standard replaces GB/T 14698-2002 “Identification method of feed
material by microscopy”. As compared with GB/T 14698-2002, the main
technical changes of this standard are as follows.
- DELETE the relevant contents of compound feed from the text (SEE clause
1 of 2002 version);
- ADJUST the standard text structure;
- ADD the petroleum ether degreasing treatment method (SEE clause 7.2.3).
This standard was proposed by AND shall be under the jurisdiction of National
Feed Industry Standardization Technical Committee (SAC/TC 76).
The drafting organizations of this standard. National Feed Quality Supervision
and Inspection Center (Wuhan), New Hope Liuhe Co., Ltd., Guangzhou
Kangruide Biological Technology Co., Ltd., Hunan Zhenghong Technology
Development Co., Ltd.
The main drafters of this standard. Yang Haipeng, Guo Jiyuan, Liu Xianrong,
Yang Lin, Rong Jia, Zhu Zhengpeng, Hu Zhenjun, Wang Hu, Qian Ying, Jiang
This standard replaces the standards previously issued as follows.
- GB/T 14698-1993, GB/T 14698-2002.
Identification method of feed material by microscopy
1 Scope
This standard specifies the identification method of feed material by microscopy.
This standard applies to the qualitative identification of feed material by
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) are applicable to this document.
GB/T 6682 Water for analytical laboratory use - Specification and test
GB/T 14699.1 Feed - Sampling
GB/T 34269-2017 Identification chromatogram of feed material by
Feed material directory (Announcement of Ministry of Agriculture of People's
Republic of China No. 1773)
3 Principles
The appearance morphology, organization structure, cell morphology, and
staining characteristics of the substance under inspection are observed under
the microscope, the results are compared with GB/T 34269-2017, to identify
and evaluate its type and quality.
4 Instruments
4.1 Stereo-microscope. magnification can be 7 times ~ 40 times.
4.2 Biological microscope. magnification can be 40 times ~ 500 times.
4.3 Magnifying glass.
a spoon to take some of specimens from above and below each sieve,
respectively LAY it horizontally in the culture dish. If necessary, the specimen
can be sieved after being treated by petroleum ether, acetone, carbon
tetrachloride (In accordance with clause 7.2.3, 7.2.4 and 7.2.5).
7.2.2 Particle or pellet specimen treatment
TAKE a few grains into a mortar (4.5), USE pestle to grind it to scatter it into
different compositions, BUT do not CRUSH the composition itself. After initial
grinding, MAKE it pass the sieve of bore diameter 0.42 mm. In accordance with
the characteristics of the feed specimen after grinding, MAKE treatment in
accordance with 7.2.3, 7.2.4 and 7.2.5.
7.2.3 Petroleum ether degreasing treatment
For the specimen of high fat content or attached with a large number of fine
particle samples (such as. fish meal, meat and bone meal, extruded soybean
and other raw materials feed samples), TAKE about 5 g of sample into a 100
mL high beaker, ADD 50 mL of petroleum ether (5.2), STIR it for 10 s, LET it be
standing to allow it to settle, carefully DECANT the petroleum ether, after the
petroleum ether at the sample surface is volatilized, PLACE it into the oven (4.9)
at about 70 °C to bake it for 10 min with the door opened, or PLACE it into a
fume hood to blow it dry, TAKE it out and COOL it to room temperature, PLACE
the sample into the culture dish (4.7) to prepare for inspection.
WARNING - This procedure shall be operated in a ventilated environment
or fume hood, beware of explosion and fire.
7.2.4 Acetone treatment
For the specimen having a lump structure due to molasses OR having high
moisture content and being vague, it can be treated first using this method.
TAKE about 10 g of specimen, PLACE it into a 100 mL high beaker, ADD about
70 mL of acetone solution (5.3), STIR it for a few minutes to dissolve the
molasses, LET it be standing to allow it to settle. Carefully DECANT it, USE the
acetone solution (5.3) to make repeated rinsing, settlement, and decantation
for two times. After it is slightly evaporated dry, PLACE it into a 60 °C oven for
20 min, TAKE it out, COOL it at room temperature.
WARNING - This procedure shall be operated in a ventilated environment
or fume hood, taking care to prevent organic solvent poisoning.
7.2.5 Carbon tetrachloride flotation treatment
TAKE about 10 g of specimen into a 100 mL high beaker, ADD about 90 mL of
carbon tetrachloride (5.1), STIR it for about 10 s, LET it be standing for 2 min ~
5 min. After the upper and lower layers are clearly separated, USE spoon to
During observation, USE a shape tweezers to toggle and flip, and USE a probe
to touch the specimen particle, to systematically inspect each composition in
the culture dish.
To facilitate observation, the ninhydrin test (9.2.6), the phloroglucinol test (9.2.7),
the iodine test (9.2.8) and the like may be performed on the specimen. During
the inspection process, MAKE comparison observation between the reference
sample and the specimen under inspection at the same conditions, or otherwise
MAKE reference to GB/T 34269 to perform comparative observation.
RECORD the various components observed, for the substance which is not
indicated by the specimen, if it is in small amount, it is called impurity, if it is in
large amount, it is called dopant. It shall pay special attention to hazardous
8.3 Biological microscopy
Specimen particles and specimen that cannot be accurately identified under a
stereomicroscope, respectively TAKE a small amount of specimen from above
the sieve and from the sieve base plate, PLACE it on the slide glass (4.7), ADD
two drops of a suspending agent I (5.11), USE the probe (4.8) to stir it to scatter
it, MAKE it uniformly soaked, USE a glass slide to cover it. Stir and disperse
with the probe (4.8), soaked and covered with a glass slip. MAKE observation
under a biological microscope (4.2), MAKE searching observation under a
lower magnification microscope first, then INCREASE the observation
magnification further for each target. COMPARE it with the reference sample.
TAKE off the glass slide (4.7), LIFT up the cover slip, ADD one drop of iodine
solution (5.7), STIR it uniformly, ADD the glass slip again, PLACE it under
microscope for observation. At this time, the starch is dyed blue to black, yeast
and other protein cells are yellow to brown. If sample transparency is too low to
be observed easily, it may take a small amount of specimen, ADD about 5 mL
of suspending agent II (5.12), MAKE it boiling for 1 min, COOL it down, TAKE
1 ~ 2 drops of bottom sediment...
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.