GB/T 13092-2006 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 13092-2006 | English | 150 |
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Enumeration of molds count in feeds
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| GB/T 13092-1991 | English | 199 |
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Method for determination of Molds in feeds
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GB/T 13092-2006: Enumeration of molds count in feeds---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT13092-2006
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.120
B 46
Replace GB/T 13092-1991
Enumeration of molds count in feeds
Issued on. JUNE 9, 2006
Implemented on. SEPTEMBER 1, 2006
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of PRC;
Standardization Administration of PRC.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Terms and definitions... 4
4 Principles... 4
5 Equipment and materials... 5
6 Media and reagents... 5
7 Determination procedure... 6
8 Preparation of samples... 7
9 Analysis steps... 7
10 Calculation... 7
Appendix A (Normative) Salt Czapek Dox Agar... 9
Foreword
This standard is a revision of GB 13092-1991 “Method for determination of molds in
feeds”.
The main differences between this standard and GB 13092-1991 are as follows.
--- The fineness of grinding in the inspection procedure is added and stipulated;
--- The specific specifications of “Equipment and materials” are added and
standardized;
--- The “Calculation method” is modified according to GB/T 4789.2-2003
“Microbiological examination of food hygiene - Detection of aerobic bacterial
count”;
--- In order to be consistent with international and domestic standards, the unit of the
molds count is changed to cfu per gram (or per milliliter), that is, cfu/g(mL).
GB 13092-1991 shall be abolished simultaneously from the date of implementation of
this standard.
Appendix A of this standard is a normative appendix.
This standard is proposed by and shall be under the jurisdiction of the National
Technical Committee on Feed Industry of Standardization Administration of China.
Drafting organizations of this standard. China National Feed Quality Inspection and
Testing Center (Beijing).
The main drafters of this standard. Li Libei, Rao Zhenghua, Yang Shuming, Su Xiaoou.
This standard was first issued in 1986 and revised for the first time in 1991.
Enumeration of molds count in feeds
1 Scope
This standard specifies the method for the determination of the molds count in feeds.
This standard applies to the determination of the molds count in feeds.
2 Normative references
The following documents contain the provisions which, through normative reference in
this document, constitute the essential provisions of this document. For the dated
referenced documents, only the versions with the indicated dates are applicable to this
document; for the undated referenced documents, only the latest version (including all
the amendments) is applicable to this document.
GB/T 4789.2-2003 Microbiological examination of food hygiene - Detection of
aerobic bacterial count
GB/T 6682-1992 Water for analytical laboratory use - Specification and test methods
GB/T 14699.1 Feeding stuffs - Sampling
3 Terms and definitions
The following terms and definitions apply to this standard.
4 Principles
According to the physiological characteristics of the mold, a medium that is suitable for
the growth of the mold but not suitable for the growth of the bacteria shall be selected,
and the number of the mold shall be determined by using the plate count method.
5 Equipment and materials
5.1 Analytical balance. The sense quantity shall be 0.001 g.
5.2 Constant temperature incubator. (25~28) °C±1 °C.
5.7 Micro mixer. 2900 r/min.
5.8 Sterilized glass Erlenmeyer flask. 250 mL, 500 mL.
5.9 Sterilized test tube. 15 mm×150 mm.
5.10 Sterilized plate. 90 mm in diameter.
5.11 Sterilized pipette. 1 mL, 10 mL.
5.12 Sterilized glass beads. 5 mm in diameter.
5.13 Sterilized jar. 100 mL, 500 mL.
5.14 Sterilized metal spoons, knives, etc.
6 Media and reagents
Unless otherwise specified, the reagents used are all analytical grade; the water shall
conform to the third-grade water specified in GB/T 6682-1992.
6.1 Salt Czapek Dox Agar. See Appendix A for the preparation method.
7 Determination procedure
The mold determination procedure is shown in Figure 1.
8 Preparation of samples
Sampling shall be carried out in accordance with GB/T 14699.1 method, and special
attention must be paid to selecting representative samples and to avoiding
contamination during sampling. First, prepare the sterilized containers and sampling
tools, such as sterilized kraft paper bags or jars, metal spoons, and knives.
9 Analysis steps
9.1 Weigh 25 g (or 25 mL) of the test sample by sterile operation, put it into a glass
Erlenmeyer flask containing 225 mL of sterilized diluent, place it on an oscillator, and
shake it for 30 min to obtain a 1.10 diluent.
9.2 Pipette 10 mL of the 1.10 diluent with a sterilized pipette, inject it into a test tube
with glass beads, place it on a micro-mixer and mix for 3 minutes; or inject it into a test
tube, blow and suck it 50 times repeatedly with a 1 mL sterilized pipette with a rubber
nipple to disperse the mold spores.
10 Calculation
10.1 Usually, the plate with molds between 10~100 is selected for counting. The
average number of molds in 2 plates with the same dilution is multiplied by the dilution
factor, the result is the molds count contained in each gram (or per milliliter) of the
sample.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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