GB 5009.169-2016 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5009.169-2016 | English | 85 |
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National food safety standard - Determination of taurine in foods
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| GB/T 5009.169-2003 | English | 279 |
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Determination of taurine in foods
| Obsolete |
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GB 5009.169-2016: National food safety standard - Determination of taurine in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.169-2016
GB
NATIONAL STANDARD
National food safety standard
Determination of taurine in foods
Issued on. AUGUST 31, 2016
Implemented on. MARCH 01, 2017
Issued by. National Health and Family Planning Commission of the PRC
Table of Contents
Foreword... 3
1 Scope... 4
2 Principles... 4
3 Reagents and materials... 4
4 Instruments and equipment... 6
5 Analytical procedures... 6
6 Expression of analysis results... 9
7 Precision... 9
8 Other... 9
9 Principles... 10
10 Reagents and materials... 10
11 Instruments and equipment... 11
12 Analytical procedures... 12
13 Expression of analysis results... 14
14 Precision... 15
15 Other... 15
Appendix A Chromatogram... 16
Foreword
This standard replaces GB/T 5009.169-2003 “National food safety standard -
Determination of taurine in foods” and GB 5413.26-2010 “National food safety
standard - Determination of taurine in foods for infants and young children, milk
and milk products”.
As compared with GB/T 5009.169-2003, the main changes of this standard are
as follows.
- CHANGE the standard name into “National food safety standard -
Determination of taurine in foods”;
- ADD the OPA post-column derivatization high performance liquid
chromatography method as the first method; ADD the dansyl chloride
pre-column derivatization high performance liquid chromatography as the
second method;
- DELETE the thin layer chromatography.
National food safety standard
Determination of taurine in foods
1 Scope
This standard specifies the method for the determination of taurine in food.
This standard applies to the determination of taurine in infant formula, milk
powder, soybean meal, soy milk, milk beverage, special purpose drink, flavor
beverage, solid beverage and jelly.
Method 1.O-phthalaldehyde (OPA) post-column derivatization high
performance liquid chromatography
2 Principles
USE water to dissolve the sample; USE the metaphosphoric acid to precipitate
the protein; after extracted by ultrasonic shock, centrifuged, and filtered
through a microporous membrane, MAKE it pass through the sodium ion
chromatography column for separation AND be subjected to o-phthalaldehyde
(OPA) derivative reaction; USE the fluorescence detector to conduct detection;
and USE the external standard method for quantitation.
3 Reagents and materials
Unless otherwise stated, the reagents used in this method are of analytical
grade AND water is level I water as specified in GB/T 6682.
3.1 Reagents
3.1.1 Metaphosphoric acid.
3.1.2 Trisodium citrate.
3.1.7 Potassium hydroxide.
3.1.8 O-phthalaldehyde (OPA).
3.1.9 2-mercaptoethanol.
3.1.10 Polyoxyethylene lauric acid ether (Brij-35).
3.2 Reagent preparation
3.2.1 Metaphosphoric acid solution (30 g/L)
WEIGH 30.0 g of metaphosphoric acid (3.1.1); USE water to dissolve it and
MAKE its volume reach to 1000 mL.
3.2.2 Trisodium citrate solution
WEIGH 19.6 g of trisodium citrate (3.1.2); ADD 950 mL of water to dissolve it;
ADD 1 mL of phenol (3.1.3); USE nitric acid (3.1.4) to adjust the pH to 3.10 ~
3.25; MAKE it pass through a 0.45 μm microfiltration membrane for filtration.
3.2.3 Post-column fluorescence derivative solution (o-phthalaldehyde
solution)
3.2.4 Precipitant
3.2.4.1 Precipitant I. WEIGH 15.0 g of potassium ferrocyanide (3.1.11); USE
water to dissolve it and MAKE the volume reach to 100 mL. This precipitant is
stable at room temperature for 3 months.
3.4 Standard solution preparation
3.4.1 Taurine standard stock solution (1 mg/mL)
Accurately WEIGH 0.1000 g of taurine standard substance (3.3), USE water to
dissolve it and MAKE the volume reach to 100 mL.
4 Instruments and equipment
4.1 High performance liquid chromatography. with a fluorescence detector.
4.2 Post-column reactor.
4.5 pH meter. accuracy 0.01.
4.6 Centrifuge. not less than 5000 r/min.
4.7 Microporous membrane. 0.45 μm.
4.8 Balance. sensitivity of 0.0001 g.
5 Analytical procedures
5.1 Sample preparation
Accurately WEIGH 1 g ~ 5 g (accurate to 0.01 g) of solid sample in the conical
flask; ADD 20 mL of about 40 °C warm water; SHAKE it uniformly to dissolve
the sample; PLACE it in the ultrasonic oscillator for ultrasonic extraction for 10
min. ADD another 50 mL of metaphosphoric acid solution (3.2.1); SHAKE it
uniformly.
5.2 Instrument reference conditions
5.2.1 Column. Sodium ion amino acid analysis column (25 cm × 4.6 mm) or the
equivalent.
5.2.2 Mobile phase. Trisodium citrate solution (3.2.2).
5.2.3 Mobile phase flow rate. 0.4 mL/min.
5.2.4 Fluorescence derivative solvent flow rate. 0.3 mL/min.
5.2.5 Column temperature. 55 °C.
5.3 Production of standard curve
Respectively INJECT the standard series working solution into the high
performance liquid chromatograph; DETERMINE the corresponding
chromatographic peak height or peak area; USE the concentration of the
standard working solution as the abscissa AND the response value (peak area
or peak height) as the ordinate, to draw the standard curve.
5.4 Determination of sample solution
INJECT the sample solution into the high performance liquid chromatograph to
obtain the chromatographic peak height or peak area; OBTAIN the
concentration of taurine in the test solution based on the standard curve.
6 Expression of analysis results
The taurine content in the sample is calculated in accordance with the equation
(1)
7 Precision
The absolute difference between the two independent determinations obtained
under repeatability conditions shall not exceed 10% of the arithmetic mean.
8 Other
When the sampling amount is 10.00 g, the method detection limit is 0.2
mg/100g AND the quantification limit is 0.5 mg/100g.
9 Principles
USE water to dissolve the sample; USE the potassium ferrocyanide and zinc
acetate to precipitate the protein. TAKE the supernatant and USE the dansyl
chloride for derivative reaction; MAKE the derivatives be separated by C18
reverse phase column. USE the UV detector (254 nm) or fluorescence
detector (excitation wavelength.
10 Reagents and materials
Unless otherwise stated, the reagents used in this method are of analytical
grade AND water is level I water as specified in GB/T 6682.
10.1 Reagents
10.1.1 Acetonitrile. chromatographic purity.
10.1.2 Glacial acetic acid.
10.1.7 Dansyl chloride (5-dimethylaminonaphthalene-1-sulfonyl chloride).
Chromatographic purity.
10.2 Reagent preparation
10.2.1 Hydrochloric acid solution (1 mol/L). ABSORB 9 mL of hydrochloric acid
(10.1.3); USE water to dilute it and MAKE the volume reach to 100 mL.
10.2.2 Sodium carbonate buffer (pH 9.5) (80 mmol/L).
10.2.3 Dansyl chloride solution (1.5 mg/mL). WEIGH 0.15 g of dansyl chloride
(10.1.7); USE acetonitrile (10.1.1) to dissolve it and MAKE the volume reach to
100 mL. PREPARE it before use.
10.3 Standard substance
Purity ≥ 99%, CAS. 107-35-7.
10.4 Preparation of standard solution
10.4.1 Taurine standard stock solution (1 mg/mL)
Accurately WEIGH 0.1000 g of taurine standard substance (3.3); USE water to
dissolve it and MAKE the volume reach to 100 mL.
11 Instruments and equipment
11.1 High performance liquid chromatograph. with a fluorescence detector or
an ultraviolet detector or diode array detector.
11.2 Vortex mixer.
11.3 Ultrasonic oscillator.
11.6 Microporous membrane. 0.45 μm.
11.7 Balance. sensitivity 0.0001 g and 0.001 g.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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