GB 14923-2022 (GB 14923-2010) PDF English
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Laboratory animal - Genetic quality control
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Laboratory animal -- Genetic quality control of mammalian laboratory animals
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Laboratory animal.Genetic quality control of mammalian laboratory animals
ICS 65.020.30
B44
National Standards of People's Republic of China
Replace GB 14923-2001
Experimental animal
Genetic quality control of mammalian experimental animals
Released on.2010-12-23
2011-10-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration issued
Foreword
Chapter 3 of this standard (except for the example), 4.1.1, 4.1.2, 4.2.1, 4.2.2, 4.2.3, 5.1, 5.3 are mandatory, and the rest are
Recommended.
This standard replaces GB 14923-2001 "Genetic quality control of experimental animal mammalian experimental animals".
Compared with GB 14923-2001, the main changes of this standard are as follows.
a) Increase the content of special inbred lines such as chromosome replacement system and nuclear transfer system, which is consistent with the latest international version of the authoritative document;
b) an addition to the description of genetically modified animals;
c) testing requirements for closed-group animals;
d) supplement the breeding method of the hybrid group;
e) The appendix has been revised.
Appendix A of this standard is a normative appendix, and Appendix B and Appendix C are informative appendices.
This standard is proposed and managed by the National Laboratory Animal Standardization Technical Committee.
This standard was drafted. National Laboratory Animal Standardization Technical Committee.
The main drafters of this standard. Yue Bingfei, Gao Xiang, Bao Shimin, Zhang Lianfeng, Xing Ruichang.
This standard was first published in January.1994 and first revised in August.2001.
Experimental animal
Genetic quality control of mammalian experimental animals
1 Scope
This standard specifies the genetic classification and naming principles of mammalian experimental animals, reproductive mating methods and genetic quality of inbred animals.
standard.
This standard is applicable to the genetic classification, naming, reproduction and genetic purity testing of inbred mouse and rat in mammalian experimental animals.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Inbred strain inbredstrain
In an animal population, more than 99% of alleles in any individual genome are homozygous and are defined as inbred lines.
Classic inbreds are bred by at least 20 consecutive generations of siblings. All individuals in the line can be traced back to the origin
A common ancestor of the 20th generation or later algebra.
Parental mating for more than 20 generations has the same effect as mating with siblings. Inbreeding coefficient of inbred lines
The coefficient) should be greater than 99%.
2.2
Substrain
Differences in genetic differentiation between animals in each branch of an inbred line are called sublines of inbred lines.
2.3
Recombinant inbred recombinantinbredstrain
RI
An inbred line that is bred by two or more generations of brothers and sisters after crossing two inbred lines.
2.4
Recombinant recombinantcongenicstrain
RC
After crossing two inbred lines, the offspring and the two inbred lines of the two parental inbreds are backcrossed several times (usually backcrossed twice) and then
An inbred line that is not bred by consecutive siblings of a particular gene (usually greater than 14 generations).
2.5
Homologous mutation inbred coisogenicinbredstrain
Except for alleles at a particular locus, all other inbred lines with the same genetics are identical.
Gene mutations or artificial mutagenesis (such as gene knockout) are generally formed by inbred lines. Using inbreeding algebra to represent the algebra of the mutation,
For example, F110 F23 is an inbred line that has been inbred for 23 generations after a mutation in the 110th generation.
2.6
Homologous introduction of inbred congenicinbredstrain
Similar inbreds
An inbred line formed by backcrossing differs only in a small chromosome segment.
New inbreds.
At least 10 generations are required to be returned, and the genome of the donor line accounts for less than 0.01 of the total genome.
2.7
Chromosome substitution system consomicstrainsorchromosomesubstitutionstrains
An inbred line that is bred by backcrossing to introduce a certain chromosome into the inbred line. Same as similar systems, with F1 as
The first generation requires at least 10 generations to return.
2.8
Nuclear transfer system conplasticstrains
A line that is cultivated by moving the nuclear genome of a line to the cytoplasm of other lines.
2.9
Mixed system mixedinbredstrains
An inbred line produced by mixing two parental lines, one of which is a recombinant gene ES cell line.
2.10
Inter-internal system
Two inbred lines are propagated to F2, and multiple inbred lines obtained by avoiding the mating of brothers and sisters are taken. Due to its higher similarity
The recombination rate between sites was applied to the fine mapping analysis of the mutant gene.
2.11
Genetically modified animal genetic modifiedanimals
Animals established by artificially induced mutations or specific types of genome modifications. Including transgenic animals, genetically-targeted mutant animals, and induced changes
Things and so on.
2.12
Closed group closedcolony
Outreach group outbredstock
An experimental animal population that is produced by non-intimate mating, at least continuously without introducing new individuals from the outside
Breeding groups of more than 4 generations.
2.13
Hybrid group hybrids
A population of offspring produced by crossing two different inbred lines. The first generation is referred to as F1.
3 Genetic classification and naming of experimental animals
3.1 Genetic classification
According to different genetic characteristics, experimental animals are divided into inbred lines, closed groups and hybrid groups.
3.2 Naming
3.2.1 Inbred lines
3.2.1.1 Naming
Inbred lines are generally named in uppercase English letters, and can also be named in uppercase English letters plus Arabic numerals. Symbols should be as short as possible. Such as
A system, TA1 system, etc.
3.2.1.2 Inbreeding algebra
The inbred algebra of the inbred line is represented by a capital letter F. For example, when the inbred algebra of an inbred is 87 generations,
(F87). If you are unclear about the previous algebra, only know that the recent inbreeding algebra is 25, which can be expressed as (F? 25).
3.2.1.3 Naming of sub-systems
The naming method of the sub-system is to add a slash after the name of the original line, and mark the symbol of the sub-line after the slash.
The symbols of the sub-system can be the following.
a) The English name of the abbreviation of the unit or individual that nurtures or produces the sub-system. The first letter is capitalized and the subsequent letters are lowercase. use
The abbreviation English name should be careful not to duplicate the published name. For example. A/He, which represents the Hessian of the A inbred
Department; CBA/J, a subfamily of CBA inbred lines maintained by the Jackson Institute of the United States;
b) When an inbred line maintained by a holder has more than two sub-lines, the abbreviation of the holder may be added after the number
The name indicates the subfamily. Such as. C57BL/6J, C57BL/10J respectively represent the C57BL near by the Jackson Institute of the United States
Two subfamilies of the lineage;
c) A sub-system is preserved in other institutions, forming a new group, and an abbreviation of the institution is added after the original sub-system. Such as. C3H/HeH is made up of
Hanston (He) subfamily preserved by Hanwel (H);
d) The exceptions to the above naming methods are some inbred lines that were established and named earlier and are well known.
It can be represented by lowercase English letters, such as BALB/c, C57BR/cd, etc.
3.2.1.4 Renaming inbred lines and recombination of similar systems
3.2.1.4.1 Naming of recombinant inbred lines
In the middle of the abbreviated names of the two parental inbred lines, the English letter X is added. a group of inbred lines bred by the same parents
The Arabic numerals are distinguished, with the female parent first and the male parent behind.
Example.
A group of recombinant inbred lines bred by two inbred lines BALB/c and C57BL, named CXB1 and CXB2, respectively.
If the male parent is abbreviated as a number, such as CX8, to distinguish between different RI groups, the connector is represented as CX8-1, CX8-2.
The abbreviated names of commonly used inbred mice are as follows.
Inbred abbreviated name
C57BL/6 B6
BALB/c C
DBA/2 D2
C3H C3
CBA CB
3.2.1.4.2 Renaming the naming of similar systems
In the middle of the abbreviated names of the two parental inbred lines, the lowercase English letter c is named, and the parental inbred line is used as the backcross.
The crossover is in the front, and the donor inbred is in the back. A group of recombinant homologs raised by the same parent is distinguished by Arabic numerals. Such as CcS1, indicating
BALB/c(C) was used as the parental inbred line, STS(S) line was used as the donor inbred line, and the number 1 was recombined after 2 generations of backcrossing.
Class.
Similarly, if the male parent is abbreviated as a number, such as Cc8, to distinguish between different RC groups, the connector is represented as Cc8-1.
3.2.1.5 Naming of homologous mutant inbred lines
Add the mutated gene symbol (in English italic print) after the name of the inbred strain that is mutated, separated by a connection number.
Such as. DBA/Ha-D, indicating that the DBA/Ha strain mutant gene is a homologous mutant inbred line of D.
When the mutant gene must be maintained in a heterozygous form, the "" sign represents the wild type gene, such as. A/Fa- /c.
129S7/SvEvBrd-Fyntm1Sor represents Fyn gene variation made with AB1...
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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