GB/T 36858-2018 English PDFUS$119.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36858-2018: Determination of aflatoxin B1 in feeds -- High performance liquid chromatography Status: Valid
Basic dataStandard ID: GB/T 36858-2018 (GB/T36858-2018)Description (Translated English): Determination of aflatoxin B1 in feeds -- High performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 6,682 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36858-2018: Determination of aflatoxin B1 in feeds -- High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of aflatoxin B1 in feeds - High performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Determination of aflatoxin B1 in feed High performance liquid chromatography Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76). This standard was drafted. Huazhong Agricultural University. The main drafters of this standard. Qi Desheng, Zhang Niya, Zhang Jiacai, Sun Alumine, Wang Jun, Tao Can, Wang Shuai. Determination of aflatoxin B1 in feed High performance liquid chromatography Caution - Aflatoxin B1 is a highly carcinogenic substance and should be handled with care. The analysis should be carried out in a fume hood or in a designated area. Row. During the analysis, the operator should take appropriate protective measures. After the analysis process, the contacted vessel and aflatoxin B1 are dissolved. The solution should be soaked overnight with a 5% sodium hypochlorite solution.1 ScopeThis standard specifies high performance liquid chromatography for the determination of aflatoxin B1 in feed. This standard applies to the determination of aflatoxin B1 in feed ingredients, compound feed, concentrated feed, and concentrate supplements. The detection limit of the method is 0.5 μg/kg, and the limit of quantification is 2.0 μg/kg.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 14699.1 Feed sampling Preparation of GB/T 20195 animal feed samples3 PrincipleThe aflatoxin B1 in the sample is extracted by the aflatoxin B1 extraction solution, then extracted by chloroform and derivatized with trifluoroacetic acid. The aflatoxin B1 after birth was measured by reversed-phase high performance liquid chromatography-fluorescence detector and quantified by external standard method.4 reagents or materialsOnly reagents identified as analytically pure were used in the analysis unless otherwise specified. 4.1 Water. It meets the primary water requirements specified in GB/T 6682. 4.2 Methanol. chromatographic grade. 4.3 Acetonitrile. chromatographic grade. 4.4 Trichloromethane. 4.5 Aflatoxin B1 extraction solution. Measure 84 mL of acetonitrile, add to 16 mL of water, and mix. 4.6 Aflatoxin B1 Derivatization Solution. Measure 20mL of trifluoroacetic acid separately, add 70mL of water, mix and add 10mL of ice B Acid, mix well. Available now. 4.7 Mobile phase. Take 20 mL of methanol, 10 mL of acetonitrile and 70 mL of water, respectively, and mix. After filtering through a 0.22 μm organic filter, it was used. 4.8 Aflatoxin B1 standard stock solution (1000μg/mL). accurately weigh aflatoxin B1 reference substance (purity ≥98%) 10.0mg, Completely dissolved in 10 mL of acetonitrile to prepare a standard stock solution with aflatoxin B1 content of 1000 μg/mL, stored at -20 ° C, effective The period is 6 months. Or certified standard solution. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36858-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36858-2018_English as soon as possible, and keep you informed of the progress. 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