GB/T 36781-2018 English PDF

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GB/T 36781-2018: Detection and identification of cucurbit seed transmitted viruses
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36781-2018	359	Add to Cart	4 days	Detection and identification of cucurbit seed transmitted viruses	Valid

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Basic data

Standard ID: GB/T 36781-2018 (GB/T36781-2018)
Description (Translated English): Detection and identification of cucurbit seed transmitted viruses
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 18,194
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36781-2018: Detection and identification of cucurbit seed transmitted viruses

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of cucurbit seed transmitted viruses ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of melon seeds Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Ningxia Entry-Exit Inspection and Quarantine Bureau, Zhengzhou Fruit Research Institute of Chinese Academy of Agricultural Sciences, China Academy of Inspection and Quarantine. The main drafters of this standard. Chen Hongyun, Chen Qing, Chen Lin, Liao Furong, Fang Zhipeng, Huang Feng, Gu Qinsheng, Zhang Yongjiang, Lin Shiming. Method for quarantine and identification of melon seeds

1 Scope

This standard specifies five common species-transmitted viruses on cucumber crops--cucumber green mottle mosaic virus (Cucumbergreenmottle) Mosaicvirus, CGMMV), Cucumbermosaicvirus (CMV), Melon Necrotic Spot Virus (Melonnec- Roticspotvirus, MNSV), Squash mosaic virus (SqMV) and small zucchini yellow mosaic virus (Zucchiniyellowmosaicvirus, ZYMV) detection method, which specifies the potato virus family (Potyviridae), tobacco mosaic A molecular identification method for the genus Tobamovirus and the southern bean genus Sobemovirus. This standard applies to watermelon (Citrullus vulgaris), melon (Cucumismelo), cucumber (Cucumissativus), pumpkin (Cu- Curbitamoschata), Lagenariasiceraria, Cucurbitapepo, Cucurbitamaxima, Quarantine and identification of seeds of melon seeds such as Momordicacharantia and Luffacylindrica.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 Principle

Serological and genomic characteristics are the main basis for quarantine identification. See Appendix A for background information on melon-type virus transmission. 4 instruments, reagents and consumables 4.1 Instruments and appliances Microplate reader, plate washer, PCR instrument, gel imaging system, constant temperature water bath, low temperature refrigerator, ordinary refrigerator, centrifuge, electronic balance, electrophoresis Instruments, electrophoresis tanks, micropipettes, mortars, etc. 4.2 Reagents and consumables See Appendix B for enzyme-linked immunosorbent assay (ELISA) reagents and Appendix C for RT-PCR assay reagents. Various sizes of tips, centrifugation Tube, enzyme plate, PCR tube, etc.

5 Quarantine and identification

5.1 Sampling Performed in accordance with SN/T 2122. 5.2 Sample preparation Seeds are sown in sterilized soil. After growing 3 to 4 true leaves, the plants showing symptoms are numbered separately, and the plants without symptoms are grown. ......

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