GB/T 36771-2018 English PDF

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GB/T 36771-2018: Detection and identification of Tomato mosaic virus
Status: Valid

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GB/T 36771-2018	439	Add to Cart	4 days	Detection and identification of Tomato mosaic virus	Valid

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Basic data

Standard ID: GB/T 36771-2018 (GB/T36771-2018)
Description (Translated English): Detection and identification of Tomato mosaic virus��
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 22,255
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36771-2018: Detection and identification of Tomato mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Tomato mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Tomato mosaic virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Content

Foreword I 1 Scope 1 2 Normative references 1 3 virus basic information 1 4 Principle 1 5 Instruments, utensils and reagents 1 5.1 Instrument 1 5.2 Appliance 2 5.3 Reagent 2 6 sampling 2 6.1 Seed sampling 2 6.2 Planting samples during growth period 3 7 Test identification 3 7.1 Double antibody sandwich enzyme-linked immunosorbent assay 3 7.2 Dot enzyme-linked immunosorbent assay 3 7.3 Colloidal gold immunochromatographic assay 3 7.4 RT-PCR detection 3 7.5 Real-time fluorescent RT-PCR detection 3 7.6 Inoculation reaction 3 8 result judgment 3 9 Sample Storage and Results Record 3 9.1 Sample storage 3 9.2 Result Record 4 Appendix A (informative) Tomato mosaic virus other information 5 Appendix B (normative appendix) Double antibody sandwich enzyme-linked immunosorbent assay 7 Appendix C (Normative) Spotted enzyme-linked immunosorbent assay 10 Appendix D (Normative) Colloidal gold immunochromatographic assay 12 Appendix E (Normative) RT-PCR detection 14 Appendix F (Normative) Real-time fluorescent RT-PCR detection 16 Appendix G (informative) Seed infection level and sampling volume 17 Appendix H (informative) Inoculation response 18 Reference 19

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people And the Gansu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Wei Meisheng, Kong Jun, Zhu Shuifang, Guo Jingze, Li Guifen, Zhao Wenjun, Wang Junping, Wen Zhaohui, You Jia. Tomato mosaic virus quarantine and identification method

1 Scope

This standard specifies the quarantine and identification methods for tomato mosaic virus (Tomatomosaicvirus). This standard applies to the detection and identification of tomato mosaic virus in host plants and untreated seeds. In order to reduce the infection rate of seeds, physical (such as heat treatment) or chemical treatment (such as acid treatment, sodium hypochlorite, trisodium phosphate, etc.) Seeds, or seeds treated with agrochemicals or biological agents, use this standard to prove the residue by analysis, sampling or comparative experiments. The inhibitors left will not affect the experiment.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 virus basic information

Scientific name. Tomatomosaicvirus. Abbreviation. ToMV. Chinese name. Tomato mosaic virus. Synonym. Lycopersicumvirus 1 (Lycopersicumvirus 1). Taxonomic status. Virgaviridae, Tobamovirus. See Appendix A for additional information on ToMV.

4 Principle

The protein and genomic characteristics of ToMV are the basis for the detection and identification of this virus. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA), colloidal gold immunochromatographic assay, RT-PCR, real-time fluorescence RT- A method such as PCR is used to specifically detect the virus and determine whether the sample has ToMV. Infectivity of ToMV is an important indicator of the biological activity of the virus. The biological vaccination method is used to judge the activity of the virus. 5 instruments, utensils and reagents 5.1 Instrument Electronic balance (sensitivity 0.001g). High-speed refrigerated centrifuge (maximum speed 15000r/min). Small instantaneous centrifuge (maximum speed 7000r/min). Ordinary refrigerator (4 ° C). Ultra-low temperature refrigerator (-80 ° C). ......

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