GB/T 36780-2018 English PDF

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GB/T 36780-2018: Detection and identification of Pepper mild mottle virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36780-2018	439	Add to Cart	4 days	Detection and identification of Pepper mild mottle virus	Valid

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Basic data

Standard ID: GB/T 36780-2018 (GB/T36780-2018)
Description (Translated English): Detection and identification of Pepper mild mottle virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 22,251
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36780-2018: Detection and identification of Pepper mild mottle virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pepper mild mottle virus ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of pepper mild mottle virus Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Content

Foreword I 1 Scope 1 2 Normative references 1 3 virus basic information 1 4 Principle 1 5 Instruments, utensils and reagents 1 5.1 Instrument 1 5.2 Appliance 2 5.3 Reagent 2 6 sampling 2 6.1 Seed sampling 2 6.2 Planting samples during growth 2 7 Test identification 3 7.1 Double antibody sandwich enzyme-linked immunosorbent assay 3 7.2 Dot enzyme-linked immunosorbent assay 3 7.3 Colloidal gold immunochromatographic assay 3 7.4 RT-PCR detection 3 7.5 Real-time fluorescent RT-PCR detection 3 7.6 Inoculation reaction 3 8 result judgment 3 9 Sample Storage and Results Record 3 9.1 Sample storage 3 9.2 Result Record 3 Appendix A (informative appendix) pepper light mottle virus other information 4 Appendix B (normative appendix) Double antibody sandwich enzyme-linked immunosorbent assay 6 Appendix C (normative appendix) Dot enzyme-linked immunosorbent assay 9 Appendix D (Normative) Colloidal gold immunochromatographic assay 11 Appendix E (Normative) RT-PCR detection 13 Appendix F (Normative) Real-time fluorescent RT-PCR detection 15 Appendix G (informative) Seed infection levels and sampling volumes 16 Appendix H (informative) Vaccination response 17 Reference 19

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people And the Gansu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Wei Meisheng, Guo Jingze, Zhu Shuifang, Kong Jun, Li Guifen, Zhao Wenjun, Wang Junping, Wen Zhaohui, You Jia. Method for quarantine and identification of pepper mild mottle virus

1 Scope

This standard specifies the quarantine and identification methods for Peppermildmottlevirus. This standard applies to the detection of pepper mild mottle virus on host plants and untreated seeds.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 virus basic information

Scientific name. Peppermildmottlevirus Abbreviation. PMMoV Chinese name. pepper light mottle virus Synonym. Tobacco mosaic virus latent strain; Tobacco mosaic virus Samsun latent strain Taxonomic status. Virgaviridae, Tobamovirus See Appendix A for additional information on PMMoV.

4 Principle

The protein and genomic characteristics of PMMoV are the basis for the detection and identification of this virus. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA), colloidal gold immunochromatographic assay, RT-PCR, real-time fluorescence Methods such as RT-PCR are used to specifically detect the virus and determine whether the sample carries PMMoV. The invasiveness of PMMoV is an important indicator of the biological activity of the virus. The biological vaccination method is used to judge the activity of the virus. 5 instruments, utensils and reagents 5.1 Instrument Electronic balance (sensitivity 0.001g). High-speed refrigerated centrifuge (maximum speed 15000r/min). Small instantaneous centrifuge (maximum speed 7000r/min). Ordinary refrigerator (4 ° C). Ultra-low temperature refrigerator (-80 ° C). Ice machine (daily ice making capacity is 110kg). Vortex oscillator (maximum speed 2800r/min). Magnetic stirrer (stirring capacity 50mL~2000mL). ......

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